Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide
The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated. Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/19/5733 |
| _version_ | 1827680683589369856 |
|---|---|
| author | Feng Liu Lijia Zhang Xi Feng Salam A. Ibrahim Wen Huang Ying Liu |
| author_facet | Feng Liu Lijia Zhang Xi Feng Salam A. Ibrahim Wen Huang Ying Liu |
| author_sort | Feng Liu |
| collection | DOAJ |
| description | The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated. Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased. CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum. In addition, CMP-1 could increase the relative abundance of <i>Bacteroidetes</i> and reduce the relative richness of <i>Firmicutes</i> at the phylum level. CMP-1 and CMP-2 could reduce the relative abundance <i>Erysipelatoclostridum</i> at the genus level. CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota. The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods. |
| first_indexed | 2024-03-10T06:55:06Z |
| format | Article |
| id | doaj.art-897a6694559a492eabcec4eb48a32494 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:55:06Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-897a6694559a492eabcec4eb48a324942023-11-22T16:31:49ZengMDPI AGMolecules1420-30492021-09-012619573310.3390/molecules26195733Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by CyclophosphamideFeng Liu0Lijia Zhang1Xi Feng2Salam A. Ibrahim3Wen Huang4Ying Liu5College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, ChinaDepartment of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USADepartment of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, USACollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, ChinaThe effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated. Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased. CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum. In addition, CMP-1 could increase the relative abundance of <i>Bacteroidetes</i> and reduce the relative richness of <i>Firmicutes</i> at the phylum level. CMP-1 and CMP-2 could reduce the relative abundance <i>Erysipelatoclostridum</i> at the genus level. CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota. The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.https://www.mdpi.com/1420-3049/26/19/5733<i>Poria cocos</i>carboxymethyl pachymaranimmunomodulatorycyclophosphamidegut microbiota |
| spellingShingle | Feng Liu Lijia Zhang Xi Feng Salam A. Ibrahim Wen Huang Ying Liu Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide Molecules <i>Poria cocos</i> carboxymethyl pachymaran immunomodulatory cyclophosphamide gut microbiota |
| title | Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
| title_full | Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
| title_fullStr | Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
| title_full_unstemmed | Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
| title_short | Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
| title_sort | immunomodulatory activity of carboxymethyl pachymaran on immunosuppressed mice induced by cyclophosphamide |
| topic | <i>Poria cocos</i> carboxymethyl pachymaran immunomodulatory cyclophosphamide gut microbiota |
| url | https://www.mdpi.com/1420-3049/26/19/5733 |
| work_keys_str_mv | AT fengliu immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide AT lijiazhang immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide AT xifeng immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide AT salamaibrahim immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide AT wenhuang immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide AT yingliu immunomodulatoryactivityofcarboxymethylpachymaranonimmunosuppressedmiceinducedbycyclophosphamide |