In vivo volume and hemoglobin dynamics of human red blood cells.
Human red blood cells (RBCs) lose ∼30% of their volume and ∼20% of their hemoglobin (Hb) content during their ∼100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing ve...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-10-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC4191880?pdf=render |
| _version_ | 1818209941203189760 |
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| author | Roy Malka Francisco Feijó Delgado Scott R Manalis John M Higgins |
| author_facet | Roy Malka Francisco Feijó Delgado Scott R Manalis John M Higgins |
| author_sort | Roy Malka |
| collection | DOAJ |
| description | Human red blood cells (RBCs) lose ∼30% of their volume and ∼20% of their hemoglobin (Hb) content during their ∼100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing vesicles during their life in the circulation, and this process is thought to dominate the changes in the RBC physical characteristics occurring during maturation. We combine theory with single-cell measurements to investigate the impact of vesiculation on the reduction in volume, Hb mass, and membrane. We show that vesicle shedding alone is sufficient to explain membrane losses but not volume or Hb losses. We use dry mass measurements of human RBCs to validate the models and to propose that additional unknown mechanisms control volume and Hb reduction and are responsible for ∼90% of the observed reduction. RBC population characteristics are used in the clinic to monitor and diagnose a wide range of conditions including malnutrition, inflammation, and cancer. Quantitative characterization of cellular maturation processes may help in the early detection of clinical conditions where maturation patterns are altered. |
| first_indexed | 2024-12-12T05:08:42Z |
| format | Article |
| id | doaj.art-897ae8bf45304754bf8295f4f3c2ca3f |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-12T05:08:42Z |
| publishDate | 2014-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-897ae8bf45304754bf8295f4f3c2ca3f2022-12-22T00:36:59ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582014-10-011010e100383910.1371/journal.pcbi.1003839In vivo volume and hemoglobin dynamics of human red blood cells.Roy MalkaFrancisco Feijó DelgadoScott R ManalisJohn M HigginsHuman red blood cells (RBCs) lose ∼30% of their volume and ∼20% of their hemoglobin (Hb) content during their ∼100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing vesicles during their life in the circulation, and this process is thought to dominate the changes in the RBC physical characteristics occurring during maturation. We combine theory with single-cell measurements to investigate the impact of vesiculation on the reduction in volume, Hb mass, and membrane. We show that vesicle shedding alone is sufficient to explain membrane losses but not volume or Hb losses. We use dry mass measurements of human RBCs to validate the models and to propose that additional unknown mechanisms control volume and Hb reduction and are responsible for ∼90% of the observed reduction. RBC population characteristics are used in the clinic to monitor and diagnose a wide range of conditions including malnutrition, inflammation, and cancer. Quantitative characterization of cellular maturation processes may help in the early detection of clinical conditions where maturation patterns are altered.http://europepmc.org/articles/PMC4191880?pdf=render |
| spellingShingle | Roy Malka Francisco Feijó Delgado Scott R Manalis John M Higgins In vivo volume and hemoglobin dynamics of human red blood cells. PLoS Computational Biology |
| title | In vivo volume and hemoglobin dynamics of human red blood cells. |
| title_full | In vivo volume and hemoglobin dynamics of human red blood cells. |
| title_fullStr | In vivo volume and hemoglobin dynamics of human red blood cells. |
| title_full_unstemmed | In vivo volume and hemoglobin dynamics of human red blood cells. |
| title_short | In vivo volume and hemoglobin dynamics of human red blood cells. |
| title_sort | in vivo volume and hemoglobin dynamics of human red blood cells |
| url | http://europepmc.org/articles/PMC4191880?pdf=render |
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