The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice
The Zika virus (ZIKV) is a mosquito-borne member of the <i>Flaviviridae</i> family of enveloped RNA viruses. The correlation between viral infection and fetal microcephaly was revealed in 2015, yet we still lack a vaccine against ZIKV. Here, we present a genetic vaccine that delivers the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/5/438 |
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| author | Hanul Choi Jungmin Chun Mina Park Suyeon Kim Nahyun Kim Hee-Jung Lee Minjee Kim Ha Youn Shin Yu-Kyoung Oh Young Bong Kim |
| author_facet | Hanul Choi Jungmin Chun Mina Park Suyeon Kim Nahyun Kim Hee-Jung Lee Minjee Kim Ha Youn Shin Yu-Kyoung Oh Young Bong Kim |
| author_sort | Hanul Choi |
| collection | DOAJ |
| description | The Zika virus (ZIKV) is a mosquito-borne member of the <i>Flaviviridae</i> family of enveloped RNA viruses. The correlation between viral infection and fetal microcephaly was revealed in 2015, yet we still lack a vaccine against ZIKV. Here, we present a genetic vaccine that delivers the premembrane (prM) and envelope (E) genes of ZIKV using a recombinant baculovirus vector that expresses a human endogenous retrovirus (HERV) envelope on its surface to enhance gene delivery. We observed that baculoviruses with HERV envelopes (AcHERV) exhibited specifically higher gene transfer efficiency in human cells compared to the wild-type baculovirus vector. Using the AcHERV baculovirus vector, we constructed a recombinant baculovirus vaccine encoding ZIKV prM/E genes (AcHERV-ZIKV), which are major targets of neutralizing antibodies. Mice immunized twice with AcHERV-ZIKV exhibited high levels of IgG, neutralizing antibodies, and IFN-γ. In challenge tests in IFN knock-out mice (A129), AcHERV-ZIKV showed complete protection in both challenge and pregnancy tests. These results suggest that AcHERV-ZIKV could be a potential vaccine candidate for human application. |
| first_indexed | 2024-03-10T11:46:21Z |
| format | Article |
| id | doaj.art-897c4962eddf44db96a9bf804aca96b4 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T11:46:21Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-897c4962eddf44db96a9bf804aca96b42023-11-21T18:03:25ZengMDPI AGVaccines2076-393X2021-04-019543810.3390/vaccines9050438The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 MiceHanul Choi0Jungmin Chun1Mina Park2Suyeon Kim3Nahyun Kim4Hee-Jung Lee5Minjee Kim6Ha Youn Shin7Yu-Kyoung Oh8Young Bong Kim9Department of Bioindustrial Technologies, Konkuk University, Seoul 05029, KoreaCenter for Glocal Disease Control, KR BioTech, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaDepartment of Biomedical Science and Engineering, Konkuk University, Seoul 05029, KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Gwanak-gu, Seoul 08826, KoreaDepartment of Bioindustrial Technologies, Konkuk University, Seoul 05029, KoreaThe Zika virus (ZIKV) is a mosquito-borne member of the <i>Flaviviridae</i> family of enveloped RNA viruses. The correlation between viral infection and fetal microcephaly was revealed in 2015, yet we still lack a vaccine against ZIKV. Here, we present a genetic vaccine that delivers the premembrane (prM) and envelope (E) genes of ZIKV using a recombinant baculovirus vector that expresses a human endogenous retrovirus (HERV) envelope on its surface to enhance gene delivery. We observed that baculoviruses with HERV envelopes (AcHERV) exhibited specifically higher gene transfer efficiency in human cells compared to the wild-type baculovirus vector. Using the AcHERV baculovirus vector, we constructed a recombinant baculovirus vaccine encoding ZIKV prM/E genes (AcHERV-ZIKV), which are major targets of neutralizing antibodies. Mice immunized twice with AcHERV-ZIKV exhibited high levels of IgG, neutralizing antibodies, and IFN-γ. In challenge tests in IFN knock-out mice (A129), AcHERV-ZIKV showed complete protection in both challenge and pregnancy tests. These results suggest that AcHERV-ZIKV could be a potential vaccine candidate for human application.https://www.mdpi.com/2076-393X/9/5/438vaccineZIKVbaculovirus systemarbovirusnonreplicated viral vector |
| spellingShingle | Hanul Choi Jungmin Chun Mina Park Suyeon Kim Nahyun Kim Hee-Jung Lee Minjee Kim Ha Youn Shin Yu-Kyoung Oh Young Bong Kim The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice Vaccines vaccine ZIKV baculovirus system arbovirus nonreplicated viral vector |
| title | The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice |
| title_full | The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice |
| title_fullStr | The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice |
| title_full_unstemmed | The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice |
| title_short | The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses against Zika Virus in A129 Mice |
| title_sort | safe baculovirus based prm e dna vaccine protected fetuses against zika virus in a129 mice |
| topic | vaccine ZIKV baculovirus system arbovirus nonreplicated viral vector |
| url | https://www.mdpi.com/2076-393X/9/5/438 |
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