In-vivo programmable acoustic manipulation of genetically engineered bacteria
Abstract Acoustic tweezers can control target movement through the momentum interaction between an acoustic wave and an object. This technology has advantages over optical tweezers for in-vivo cell manipulation due to its high tissue penetrability and strong acoustic radiation force. However, normal...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-38814-w |
| _version_ | 1797806585261588480 |
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| author | Ye Yang Yaozhang Yang Dingyuan Liu Yuanyuan Wang Minqiao Lu Qi Zhang Jiqing Huang Yongchuan Li Teng Ma Fei Yan Hairong Zheng |
| author_facet | Ye Yang Yaozhang Yang Dingyuan Liu Yuanyuan Wang Minqiao Lu Qi Zhang Jiqing Huang Yongchuan Li Teng Ma Fei Yan Hairong Zheng |
| author_sort | Ye Yang |
| collection | DOAJ |
| description | Abstract Acoustic tweezers can control target movement through the momentum interaction between an acoustic wave and an object. This technology has advantages over optical tweezers for in-vivo cell manipulation due to its high tissue penetrability and strong acoustic radiation force. However, normal cells are difficult to acoustically manipulate because of their small size and the similarity between their acoustic impedance and that of the medium. In this study, we use the heterologous expression of gene clusters to generate genetically engineered bacteria that can produce numerous sub-micron gas vesicles in the bacterial cytoplasm. We show that the presence of the gas vesicles significantly enhances the acoustic sensitivity of the engineering bacteria, which can be manipulated by ultrasound. We find that by employing phased-array-based acoustic tweezers, the engineering bacteria can be trapped into clusters and manipulated in vitro and in vivo via electronically steered acoustic beams, enabling the counter flow or on-demand flow of these bacteria in the vasculature of live mice. Furthermore, we demonstrate that the aggregation efficiency of engineering bacteria in a tumour is improved by utilizing this technology. This study provides a platform for the in-vivo manipulation of live cells, which will promote the progress of cell-based biomedical applications. |
| first_indexed | 2024-03-13T06:09:29Z |
| format | Article |
| id | doaj.art-897c96a0c8874473bb613037b1a06d67 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T06:09:29Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-897c96a0c8874473bb613037b1a06d672023-06-11T11:19:35ZengNature PortfolioNature Communications2041-17232023-06-0114111410.1038/s41467-023-38814-wIn-vivo programmable acoustic manipulation of genetically engineered bacteriaYe Yang0Yaozhang Yang1Dingyuan Liu2Yuanyuan Wang3Minqiao Lu4Qi Zhang5Jiqing Huang6Yongchuan Li7Teng Ma8Fei Yan9Hairong Zheng10Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesShenzhen Institutes of Advanced Technology, Chinese Academy of SciencesAbstract Acoustic tweezers can control target movement through the momentum interaction between an acoustic wave and an object. This technology has advantages over optical tweezers for in-vivo cell manipulation due to its high tissue penetrability and strong acoustic radiation force. However, normal cells are difficult to acoustically manipulate because of their small size and the similarity between their acoustic impedance and that of the medium. In this study, we use the heterologous expression of gene clusters to generate genetically engineered bacteria that can produce numerous sub-micron gas vesicles in the bacterial cytoplasm. We show that the presence of the gas vesicles significantly enhances the acoustic sensitivity of the engineering bacteria, which can be manipulated by ultrasound. We find that by employing phased-array-based acoustic tweezers, the engineering bacteria can be trapped into clusters and manipulated in vitro and in vivo via electronically steered acoustic beams, enabling the counter flow or on-demand flow of these bacteria in the vasculature of live mice. Furthermore, we demonstrate that the aggregation efficiency of engineering bacteria in a tumour is improved by utilizing this technology. This study provides a platform for the in-vivo manipulation of live cells, which will promote the progress of cell-based biomedical applications.https://doi.org/10.1038/s41467-023-38814-w |
| spellingShingle | Ye Yang Yaozhang Yang Dingyuan Liu Yuanyuan Wang Minqiao Lu Qi Zhang Jiqing Huang Yongchuan Li Teng Ma Fei Yan Hairong Zheng In-vivo programmable acoustic manipulation of genetically engineered bacteria Nature Communications |
| title | In-vivo programmable acoustic manipulation of genetically engineered bacteria |
| title_full | In-vivo programmable acoustic manipulation of genetically engineered bacteria |
| title_fullStr | In-vivo programmable acoustic manipulation of genetically engineered bacteria |
| title_full_unstemmed | In-vivo programmable acoustic manipulation of genetically engineered bacteria |
| title_short | In-vivo programmable acoustic manipulation of genetically engineered bacteria |
| title_sort | in vivo programmable acoustic manipulation of genetically engineered bacteria |
| url | https://doi.org/10.1038/s41467-023-38814-w |
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