Denitrification coupled with methane anoxic oxidation and microbial community involved identification
In this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2011-02-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100022 |
| _version_ | 1818541239916560384 |
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| author | Renata Medici Frayne Cuba Iolanda Cristina Duarte Nora Katia Saavedra Maria Bernadete Amâncio Varesche Eugenio Foresti |
| author_facet | Renata Medici Frayne Cuba Iolanda Cristina Duarte Nora Katia Saavedra Maria Bernadete Amâncio Varesche Eugenio Foresti |
| author_sort | Renata Medici Frayne Cuba |
| collection | DOAJ |
| description | In this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC. Denitrificant/methanotrophic biomass was taken from a laboratory scale reactor fed with synthetic nitrified substrates (40 mgN L-1 of NO3- and subsequently NO2-) and methane as carbon source. Results obtained from nitrate removal followed a first order reaction, presenting a kinetic apparent constant (kNO3)) of 0.0577±0.0057d-1. Two notable points of the denitrification rate (0.12gNO3--N g-1 AVS d-1 and 0.07gNO3--N g-1 AVS d-1) were observed in the beginning and on the seventh day of operation. When nitrite was added as an electron acceptor, denitrification rates were improved, presenting an apparent kinetic constant (kNO2) of 0.0722±0.0044d-1, a maximum denitrification rate of 0.6gNO2--N g-1AVS d-1, and minimum denitrification rate of 0.1gNO2--N g-1AVS d-1 at the beginning and end of the test, respectively. Endogenous material supporting denitrification and methane concentration dissolved in the substrate was discarded from the control experiments in the absence of methane and seed, respectively. Methylomonas sp. was identified in the reactors fed with nitrate and nitrite as well as uncultured bacterium. |
| first_indexed | 2024-12-11T22:06:02Z |
| format | Article |
| id | doaj.art-897d6cfcbbe04904aa8073957f4c9fc5 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T22:06:02Z |
| publishDate | 2011-02-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-897d6cfcbbe04904aa8073957f4c9fc52022-12-22T00:48:56ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-02-0154117318210.1590/S1516-89132011000100022Denitrification coupled with methane anoxic oxidation and microbial community involved identificationRenata Medici Frayne CubaIolanda Cristina DuarteNora Katia SaavedraMaria Bernadete Amâncio VarescheEugenio ForestiIn this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC. Denitrificant/methanotrophic biomass was taken from a laboratory scale reactor fed with synthetic nitrified substrates (40 mgN L-1 of NO3- and subsequently NO2-) and methane as carbon source. Results obtained from nitrate removal followed a first order reaction, presenting a kinetic apparent constant (kNO3)) of 0.0577±0.0057d-1. Two notable points of the denitrification rate (0.12gNO3--N g-1 AVS d-1 and 0.07gNO3--N g-1 AVS d-1) were observed in the beginning and on the seventh day of operation. When nitrite was added as an electron acceptor, denitrification rates were improved, presenting an apparent kinetic constant (kNO2) of 0.0722±0.0044d-1, a maximum denitrification rate of 0.6gNO2--N g-1AVS d-1, and minimum denitrification rate of 0.1gNO2--N g-1AVS d-1 at the beginning and end of the test, respectively. Endogenous material supporting denitrification and methane concentration dissolved in the substrate was discarded from the control experiments in the absence of methane and seed, respectively. Methylomonas sp. was identified in the reactors fed with nitrate and nitrite as well as uncultured bacterium.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100022denitrificationMethylomonas spmethane oxidation |
| spellingShingle | Renata Medici Frayne Cuba Iolanda Cristina Duarte Nora Katia Saavedra Maria Bernadete Amâncio Varesche Eugenio Foresti Denitrification coupled with methane anoxic oxidation and microbial community involved identification Brazilian Archives of Biology and Technology denitrification Methylomonas sp methane oxidation |
| title | Denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| title_full | Denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| title_fullStr | Denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| title_full_unstemmed | Denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| title_short | Denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| title_sort | denitrification coupled with methane anoxic oxidation and microbial community involved identification |
| topic | denitrification Methylomonas sp methane oxidation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100022 |
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