Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.
Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromos...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-06-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3688505?pdf=render |
| _version_ | 1818307310908342272 |
|---|---|
| author | Tatiana Venkova-Canova Jong Hwan Baek Peter C Fitzgerald Melanie Blokesch Dhruba K Chattoraj |
| author_facet | Tatiana Venkova-Canova Jong Hwan Baek Peter C Fitzgerald Melanie Blokesch Dhruba K Chattoraj |
| author_sort | Tatiana Venkova-Canova |
| collection | DOAJ |
| description | Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly, without nucleation at the centromere, ParB2 could also bind directly to a related 39-mer. The 39-mers are the strongest inhibitors of chromosome II replication and they mediate inhibition by binding the replication initiator protein. ParB2 thus appears to promote replication by out-competing initiator binding to the 39-mers using two mechanisms: spreading into one and direct binding to the other. We suggest that both these are novel mechanisms to coordinate replication initiation with segregation of chromosomes. |
| first_indexed | 2024-12-13T06:56:21Z |
| format | Article |
| id | doaj.art-897f17d8d8b746258d35b86c96eabb27 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-13T06:56:21Z |
| publishDate | 2013-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-897f17d8d8b746258d35b86c96eabb272022-12-21T23:56:01ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-06-0196e100357910.1371/journal.pgen.1003579Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.Tatiana Venkova-CanovaJong Hwan BaekPeter C FitzgeraldMelanie BlokeschDhruba K ChattorajUnderstanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly, without nucleation at the centromere, ParB2 could also bind directly to a related 39-mer. The 39-mers are the strongest inhibitors of chromosome II replication and they mediate inhibition by binding the replication initiator protein. ParB2 thus appears to promote replication by out-competing initiator binding to the 39-mers using two mechanisms: spreading into one and direct binding to the other. We suggest that both these are novel mechanisms to coordinate replication initiation with segregation of chromosomes.http://europepmc.org/articles/PMC3688505?pdf=render |
| spellingShingle | Tatiana Venkova-Canova Jong Hwan Baek Peter C Fitzgerald Melanie Blokesch Dhruba K Chattoraj Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. PLoS Genetics |
| title | Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. |
| title_full | Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. |
| title_fullStr | Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. |
| title_full_unstemmed | Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. |
| title_short | Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II. |
| title_sort | evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome ii |
| url | http://europepmc.org/articles/PMC3688505?pdf=render |
| work_keys_str_mv | AT tatianavenkovacanova evidencefortwodifferentregulatorymechanismslinkingreplicationandsegregationofvibriocholeraechromosomeii AT jonghwanbaek evidencefortwodifferentregulatorymechanismslinkingreplicationandsegregationofvibriocholeraechromosomeii AT petercfitzgerald evidencefortwodifferentregulatorymechanismslinkingreplicationandsegregationofvibriocholeraechromosomeii AT melanieblokesch evidencefortwodifferentregulatorymechanismslinkingreplicationandsegregationofvibriocholeraechromosomeii AT dhrubakchattoraj evidencefortwodifferentregulatorymechanismslinkingreplicationandsegregationofvibriocholeraechromosomeii |