Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas)
Diseases are one of the possible threats to the conservation of wild cat populations. One disease that has been reported to infect and cause death, including in various wildlife, is the canine distemper virus (CDV). Here, we report the first case of CDV in an adolescent melanistic Javan female leopa...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022026299 |
| _version_ | 1811213626349977600 |
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| author | Dede Aulia Rahman Uus Saepuloh Yanto Santosa Huda Shalahudin Darusman Irene Margareth Romaria Pinondang Andy Sean Kindangen Amira Putri Pertiwi Lana Sari Andi Irawan Keni Sultan Puji Rianti |
| author_facet | Dede Aulia Rahman Uus Saepuloh Yanto Santosa Huda Shalahudin Darusman Irene Margareth Romaria Pinondang Andy Sean Kindangen Amira Putri Pertiwi Lana Sari Andi Irawan Keni Sultan Puji Rianti |
| author_sort | Dede Aulia Rahman |
| collection | DOAJ |
| description | Diseases are one of the possible threats to the conservation of wild cat populations. One disease that has been reported to infect and cause death, including in various wildlife, is the canine distemper virus (CDV). Here, we report the first case of CDV in an adolescent melanistic Javan female leopard in Indonesia. We combined the clinical report with the Reverse Transcription PCR (RT-PCR) analyses on the faecal and brain samples of partial nucleoprotein (CDV-N) and hemagglutinin (CDV-H) genes. We also performed analyses of urine, haematology, and blood chemistry. The CDV-H nucleotide sequence confirmed the CDV infection in the female leopard and was clustered to the CDV's Asia 1 genotype. This finding opens an investigating window to analyse the pathogen transmission between animals in wildlife, particularly to support the management of conservation in natural habitats in Indonesia. |
| first_indexed | 2024-04-12T05:49:47Z |
| format | Article |
| id | doaj.art-897f8085205c4b9fb500a4dcdb54ef8d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T05:49:47Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-897f8085205c4b9fb500a4dcdb54ef8d2022-12-22T03:45:20ZengElsevierHeliyon2405-84402022-11-01811e11341Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas)Dede Aulia Rahman0Uus Saepuloh1Yanto Santosa2Huda Shalahudin Darusman3Irene Margareth Romaria Pinondang4Andy Sean Kindangen5Amira Putri Pertiwi6Lana Sari7Andi Irawan8Keni Sultan9Puji Rianti10Department of Forest Resources Conservation and Ecotourism, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, Indonesia; Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Javan Leopard Conservation Forum, Bogor, Indonesia; Corresponding author.Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, IndonesiaDepartment of Forest Resources Conservation and Ecotourism, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, IndonesiaPrimate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Bogor, IndonesiaJavan Leopard Conservation Forum, Bogor, Indonesia; Durrel Institute of Conservation and Ecology, School of Anthropology and Conservation, University of Kent, Canterbury, United KingdomJavan Leopard Conservation Forum, Bogor, Indonesia; Animal Sanctuary Trust Indonesia, Bogor, IndonesiaAnimal Sanctuary Trust Indonesia, Bogor, IndonesiaCenter for Conservation of Natural Resources of West Java, Ministry of Environmental and Forestry, Bandung, IndonesiaCenter for Conservation of Natural Resources of West Java, Ministry of Environmental and Forestry, Bandung, IndonesiaJavan Leopard Conservation Forum, Bogor, Indonesia; Taman Safari Indonesia, Bogor, IndonesiaPrimate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University (Bogor Agricultural University), Bogor, IndonesiaDiseases are one of the possible threats to the conservation of wild cat populations. One disease that has been reported to infect and cause death, including in various wildlife, is the canine distemper virus (CDV). Here, we report the first case of CDV in an adolescent melanistic Javan female leopard in Indonesia. We combined the clinical report with the Reverse Transcription PCR (RT-PCR) analyses on the faecal and brain samples of partial nucleoprotein (CDV-N) and hemagglutinin (CDV-H) genes. We also performed analyses of urine, haematology, and blood chemistry. The CDV-H nucleotide sequence confirmed the CDV infection in the female leopard and was clustered to the CDV's Asia 1 genotype. This finding opens an investigating window to analyse the pathogen transmission between animals in wildlife, particularly to support the management of conservation in natural habitats in Indonesia.http://www.sciencedirect.com/science/article/pii/S2405844022026299Canine distemperConservationDiseasesLeopardEnvironmental health |
| spellingShingle | Dede Aulia Rahman Uus Saepuloh Yanto Santosa Huda Shalahudin Darusman Irene Margareth Romaria Pinondang Andy Sean Kindangen Amira Putri Pertiwi Lana Sari Andi Irawan Keni Sultan Puji Rianti Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) Heliyon Canine distemper Conservation Diseases Leopard Environmental health |
| title | Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) |
| title_full | Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) |
| title_fullStr | Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) |
| title_full_unstemmed | Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) |
| title_short | Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas) |
| title_sort | molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard panthera pardus ssp melas |
| topic | Canine distemper Conservation Diseases Leopard Environmental health |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022026299 |
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