OBTAINING OF MONOCLONAL ANTIBODIES AGAINST CHOLERA TOXIN AND HEAT LABILE ENTEROTOXIN OF E. coli FOR DEVELOPMENT OF THE TOXINS DIPLEX ANALYSIS IN ENVIRONMENTAL SPECIMENS

OBTAINING OF MONOCLONAL ANTIBODIES AGAINST CHOLERA TOXIN AND HEAT LABILE ENTEROTOXIN OF E. coli FOR DEVELOPMENT OF THE TOXINS DIPLEX ANALYSIS IN ENVIRONMENTAL SPECIMENS

The present study focuses on development of monoclonal antibodies (MAbs) which specifically interact with cholera toxin or heat labile enterotoxin of E. coli. Such monoclonal antibodies MAbs are possessed of ability to identify cholera toxin or heat labile enterotoxin in different immunochemical ass...

Full description

Bibliographic Details
Main Authors:	Eu. V. Grishin, T. I. Valiakina
Format:	Article
Language:	English
Published:	National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 2013-08-01
Series:	Biotechnologia Acta
Subjects:	sandwich assay ELISA multiplex immunoassay xMAP analysis monoclonal antibodies minimal detectable concentration cholera toxin heat labile enterotoxin of E. coli.
Online Access:	http://biotechnology.kiev.ua/images/no4_2013/grishin.pdf

Similar Items

Cholera-Like Enterotoxins and Regulatory T cells
by: Evelyne Kohli, et al.
Published: (2010-07-01)

Effect of vibrio cholerae enterotoxin on phagocytosis in vitro
by: Baghdad Science Journal
Published: (2010-09-01)

The Suppressive Activities of Six Sources of Medicinal Ferns Known as Gusuibu on Heat-Labile Enterotoxin-Induced Diarrhea
by: Hung-Chi Chang, et al.
Published: (2014-02-01)

Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique
by: Esfandiari P, et al.
Published: (2015-09-01)

Heat-Labile Enterotoxin: Beyond G M1 Binding
by: Benjamin Mudrak, et al.
Published: (2010-06-01)

Heat-labile enterotoxin enhances F4-producing enterotoxigenic E. coli adhesion to porcine intestinal epithelial cells by upregulating bacterial adhesins and STb enterotoxin
by: Qiangde Duan, et al.
Published: (2022-10-01)

Highly Sensitive and Specific Detection of Staphylococcal Enterotoxins SEA, SEG, SEH, and SEI by Immunoassay
by: Cécile Féraudet Tarisse, et al.
Published: (2021-02-01)

Development of an Immunoassay for Detection of Staphylococcal Enterotoxin-Like J, A Non-Characterized Toxin
by: Hisaya K. Ono, et al.
Published: (2018-11-01)

Isolation and Genomic Characterization of a Heat-Labile Enterotoxin 1-Producing Escherichia fergusonii Strain from a Human
by: Miki Okuno, et al.
Published: (2023-08-01)

Heat-Labile Enterotoxin-Induced PERK-CHOP Pathway Activation Causes Intestinal Epithelial Cell Apoptosis
by: Xi Lu, et al.
Published: (2017-06-01)

Biological Activity of Recombinant Accessory Cholerae Enterotoxin (Ace) on Rabbit Ileal Loops and Antibacterial Assay
by: Shaghayegh Anvari, et al.
Published: (2012-01-01)

Multiplexed bead-based assay for the simultaneous quantification of human serum IgG antibodies to tetanus, diphtheria, pertussis toxin, filamentous hemagglutinin, and pertactin
by: Vishal Rathod, et al.
Published: (2023-06-01)

Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
by: Shahram Nazarian, et al.
Published: (2014-05-01)

Enterotoxigenic Escherichia coli heat labile enterotoxin affects neutrophil effector functions via cAMP/PKA/ERK signaling
by: Jinglin Ma, et al.
Published: (2024-12-01)

Production of monoclonal antibody of heat-labile toxin A subunit to identify enterotoxigenic Escherichia coli by epitope mapping using synthetic peptides
by: Jun-Young Park, et al.
Published: (2023-05-01)

Cell-Free Systems Enable the Production of AB<sub>5</sub> Toxins for Diagnostic Applications
by: Franziska Ramm, et al.
Published: (2022-03-01)

Different Types of Cell Death Induced by Enterotoxins
by: Ming-Yuan Hong, et al.
Published: (2010-08-01)

Designing Vaccines to Neutralize Effective Toxin Delivery by Enterotoxigenic Escherichia coli
by: James M. Fleckenstein, et al.
Published: (2014-06-01)

The Role of Vesicles in Transporting of Cholera Toxin
by: V. P. Zyuzina, et al.
Published: (2023-07-01)

A Sensitive Immunodetection Assay Using Antibodies Specific to Staphylococcal Enterotoxin B Produced by Baculovirus Expression
by: Ju-Hong Jang, et al.
Published: (2022-09-01)

Toxin Mediated Diarrhea in the 21st Century: The Pathophysiology of Intestinal Ion Transport in the Course of ETEC, V. cholerae and Rotavirus Infection
by: John P. Geibel, et al.
Published: (2010-08-01)

An LTB-entrapped protein in PLGA nanoparticles preserves against enterotoxin of enterotoxigenic Escherichia coli
by: Emad Kordbacheh, et al.
Published: (2018-05-01)

Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae
by: Cornelius C. Ssemakalu, et al.
Published: (2013-09-01)

<italic toggle="yes">In Situ</italic> Analyses Directly in Diarrheal Stool Reveal Large Variations in Bacterial Load and Active Toxin Expression of Enterotoxigenic <named-content content-type="genus-species">Escherichia coli</named-content> and <named-content content-type="genus-species">Vibrio cholerae</named-content>
by: Yasmin Ara Begum, et al.
Published: (2018-02-01)

Cholera Toxin Production and Localization in Vesicles of Vibrio cholerae El Tor Genovariants
by: L. P. Alekseeva, et al.
Published: (2024-07-01)

Carbohydrate inhibitors of cholera toxin
by: Vajinder Kumar, et al.
Published: (2018-02-01)

Effect of (−)-Epigallocatechin Gallate to Staphylococcal Enterotoxin A on Toxin Activity
by: Yuko Shimamura, et al.
Published: (2020-04-01)

Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L.) fruit
by: Nguyen Hoang LOC, et al.
Published: (2014-03-01)

Highly Pathogenic Avian Influenza H5 Hemagglutinin Fused with the A Subunit of Type IIb <i>Escherichia coli</i> Heat Labile Enterotoxin Elicited Protective Immunity and Neutralization by Intranasal Immunization in Mouse and Chicken Models
by: Neos Tang, et al.
Published: (2019-11-01)

Immunization with recombinant fusion of LTB and linear epitope (40–62) of epsilon toxin elicits protective immune response against the epsilon toxin of Clostridium perfringens type D
by: Himani Kaushik, et al.
Published: (2019-07-01)

Antibody against recombinant heat labile enterotoxin B subunit (rLTB) could block LT binding to ganglioside M1 receptor
by: J Salimian, et al.
Published: (2010-09-01)

A highly neutralizing human monoclonal antibody targeting a novel linear epitope on staphylococcal enterotoxin B
by: Hongyin Fan, et al.
Published: (2024-12-01)

Expression of Cholera Toxin (CT) and the Toxin Co-Regulated Pilus (TCP) by Variants of ToxT in <i>Vibrio cholerae</i> Strains
by: Donghyun Lee, et al.
Published: (2023-08-01)

THE STUDY OF DIAGNOSTIC POTENTIAL OF MONOCLONAL ANTIBODIES SPECIFIC TO THE MEMBRANE PROTEIN OF CHOLERA AGENT IN ENZYME-LINKED IMMUNOASSAY
by: V. V. Evdokimova, et al.
Published: (2017-09-01)

Integrating scFv into xMAP Assays for the Detection of Marine Toxins
by: Lisa C. Shriver-Lake, et al.
Published: (2016-11-01)

Cloning and Expression of <i>ace</i> Gene of <i>Vibrio cholerae</i> in <i>Escherichia coli</i>
by: E. V. Monakhova, et al.
Published: (2012-04-01)

The effect of cholera toxin and its toxoid on ileal loops of the rat
by: Ibrahim Felippe Heneine, et al.
Published: (1994-03-01)

Cholera Toxin Production in Vibrio cholerae O1 El Tor Biotype Strains in Single-Phase Culture
by: Yeongjun Baek, et al.
Published: (2020-05-01)

New Advantageous Method for the Production of Purified Cholera Toxin B-Subunit and Monoclonal Antibodies to It
by: T. L. Zakharova, et al.
Published: (2015-06-01)

Determining the immunological characteristics of a novel human monoclonal antibody developed against staphylococcal enterotoxin B
by: Yuanyuan Liu, et al.
Published: (2020-07-01)