Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat

Abstract The flag leaf is an important photosynthetic organ of wheat (Triticum aestivum L.). Appropriate flag leaf size can effectively increase grain yield. In this study, a tetraploid wheat population of recombinant inbred lines (RILs) and a genetic map constructed based on a wheat 55K single‐nucl...

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Váldodahkkit: Jian Wang, Hang Liu, Conghao Zhao, Huaping Tang, Yang Mu, Qiang Xu, Mei Deng, Qiantao Jiang, Guoyue Chen, Pengfei Qi, Jirui Wang, Yunfeng Jiang, Shisheng Chen, Yuming Wei, Youliang Zheng, Xiujin Lan, Jian Ma
Materiálatiipa: Artihkal
Giella:English
Almmustuhtton: Wiley 2022-12-01
Ráidu:The Plant Genome
Liŋkkat:https://doi.org/10.1002/tpg2.20252
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author Jian Wang
Hang Liu
Conghao Zhao
Huaping Tang
Yang Mu
Qiang Xu
Mei Deng
Qiantao Jiang
Guoyue Chen
Pengfei Qi
Jirui Wang
Yunfeng Jiang
Shisheng Chen
Yuming Wei
Youliang Zheng
Xiujin Lan
Jian Ma
author_facet Jian Wang
Hang Liu
Conghao Zhao
Huaping Tang
Yang Mu
Qiang Xu
Mei Deng
Qiantao Jiang
Guoyue Chen
Pengfei Qi
Jirui Wang
Yunfeng Jiang
Shisheng Chen
Yuming Wei
Youliang Zheng
Xiujin Lan
Jian Ma
author_sort Jian Wang
collection DOAJ
description Abstract The flag leaf is an important photosynthetic organ of wheat (Triticum aestivum L.). Appropriate flag leaf size can effectively increase grain yield. In this study, a tetraploid wheat population of recombinant inbred lines (RILs) and a genetic map constructed based on a wheat 55K single‐nucleotide polymorphism (SNP) array were used to identify quantitative trait loci (QTL) for flag leaf length (FLL), flag leaf width (FLW), flag leaf area (FLA), and the flag leaf length/width ratio (FLR). A novel and major interval flanked by markers AX‐111633224 and AX‐109317229 was identified. This interval includes QTL for FLL (QFll.sau‐AM‐4B.2), for FLW (QFlw.sau‐AM‐4B.4), for FLA (QFla.sau‐AM‐4B), and for FLR (QFlr.sau‐AM‐4B). Based on the genotypes of the closely linked KASP (Kompetitive allele‐specific polymerase chain reaction [PCR]) marker (KASP‐AX‐108756198), QFlw.sau‐AM‐4B.4 and QFla.sau‐AM‐4B were successfully verified in two F3 populations with different genetic backgrounds. Genetic associations between flag leaf‐related traits and other agronomic traits were detected and analyzed. Four genes in this interval were likely involved in the growth and development of the flag leaf size. In conclusion, this study provides clues for excavating genes related to flag leaf size and breeding variety with ideal plant structure.
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spelling doaj.art-8984d9f358584cf9a6f5762703c59bbe2022-12-22T04:41:28ZengWileyThe Plant Genome1940-33722022-12-01154n/an/a10.1002/tpg2.20252Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheatJian Wang0Hang Liu1Conghao Zhao2Huaping Tang3Yang Mu4Qiang Xu5Mei Deng6Qiantao Jiang7Guoyue Chen8Pengfei Qi9Jirui Wang10Yunfeng Jiang11Shisheng Chen12Yuming Wei13Youliang Zheng14Xiujin Lan15Jian Ma16State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaInstitute of Advanced Agricultural Sciences Peking Univ. Weifang 262113 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaTriticeae Research Institute Sichuan Agricultural Univ. Chengdu 611130 ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural Univ. Chengdu 611130 ChinaAbstract The flag leaf is an important photosynthetic organ of wheat (Triticum aestivum L.). Appropriate flag leaf size can effectively increase grain yield. In this study, a tetraploid wheat population of recombinant inbred lines (RILs) and a genetic map constructed based on a wheat 55K single‐nucleotide polymorphism (SNP) array were used to identify quantitative trait loci (QTL) for flag leaf length (FLL), flag leaf width (FLW), flag leaf area (FLA), and the flag leaf length/width ratio (FLR). A novel and major interval flanked by markers AX‐111633224 and AX‐109317229 was identified. This interval includes QTL for FLL (QFll.sau‐AM‐4B.2), for FLW (QFlw.sau‐AM‐4B.4), for FLA (QFla.sau‐AM‐4B), and for FLR (QFlr.sau‐AM‐4B). Based on the genotypes of the closely linked KASP (Kompetitive allele‐specific polymerase chain reaction [PCR]) marker (KASP‐AX‐108756198), QFlw.sau‐AM‐4B.4 and QFla.sau‐AM‐4B were successfully verified in two F3 populations with different genetic backgrounds. Genetic associations between flag leaf‐related traits and other agronomic traits were detected and analyzed. Four genes in this interval were likely involved in the growth and development of the flag leaf size. In conclusion, this study provides clues for excavating genes related to flag leaf size and breeding variety with ideal plant structure.https://doi.org/10.1002/tpg2.20252
spellingShingle Jian Wang
Hang Liu
Conghao Zhao
Huaping Tang
Yang Mu
Qiang Xu
Mei Deng
Qiantao Jiang
Guoyue Chen
Pengfei Qi
Jirui Wang
Yunfeng Jiang
Shisheng Chen
Yuming Wei
Youliang Zheng
Xiujin Lan
Jian Ma
Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
The Plant Genome
title Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
title_full Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
title_fullStr Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
title_full_unstemmed Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
title_short Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat
title_sort mapping and validation of major and stable qtl for flag leaf size from tetraploid wheat
url https://doi.org/10.1002/tpg2.20252
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