Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation

Abstract Background Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods We introduced a ro...

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Main Authors: Avery Rui Sun, Qili Sun, Yansong Wang, Liqiu Hu, Yutong Wu, Fenbo Ma, Jiayi Liu, Xiangchao Pang, Bin Tang
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS) 2023-03-01
Series:Biomaterials Research
Subjects:
Online Access:https://doi.org/10.1186/s40824-023-00361-2
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author Avery Rui Sun
Qili Sun
Yansong Wang
Liqiu Hu
Yutong Wu
Fenbo Ma
Jiayi Liu
Xiangchao Pang
Bin Tang
author_facet Avery Rui Sun
Qili Sun
Yansong Wang
Liqiu Hu
Yutong Wu
Fenbo Ma
Jiayi Liu
Xiangchao Pang
Bin Tang
author_sort Avery Rui Sun
collection DOAJ
description Abstract Background Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods We introduced a robust and simple approach of modifying titanium surfaces with polysaccharide complexes. Titanium samples were subjected to hydrothermal treatment to create a uniform porous structure on the surface, followed by coating with a bioinspired and self-assembly polydopamine layer. Strontium Eucommia Ulmoides Polysaccharide (EUP-Sr) complexes are then introduced to the polydopamine-coated porous titanium. Multiple morphological and physiochemical characterizations are employed for material evaluation, while cell proliferation and gene expression tests using macrophages, primary alveolar bone osteoblasts, and vascular endothelial cells are used to provide an overall insight into the functions of the product. The significances of statistical differences were analyzed using student’s t-test. Results Microscopic and spectrometric characterizations confirmed that the Ti surface formed a porous structure with an adequate amount of EUP-Sr loading. The attachment was attributed to hydrogen bonding between the ubiquitous glycosidic linkage of the polysaccharide complex and the ring structure of polydopamine, yet the loaded EUP-Sr complex can be gradually released, consequently benefiting the neighboring microenvironment. Cell experiments showed no cytotoxicity of the material, and the product showed promising anti-inflammation, osseointegration, and angiogenesis properties, which were further confirmed by in vivo evaluations. Conclusion We believe the EUP-Sr modified titanium implant is a promising candidate to be used in dental applications with notable osteoimmunomodulation and angiogenesis functions. And the novel technique proposed in this study would benefit the modification of metal/inorganic surfaces with polysaccharides for future research.
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spelling doaj.art-89875131b7c74d77b1fb74cd4eb478362024-03-03T10:11:39ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242023-03-0127111310.1186/s40824-023-00361-2Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammationAvery Rui Sun0Qili Sun1Yansong Wang2Liqiu Hu3Yutong Wu4Fenbo Ma5Jiayi Liu6Xiangchao Pang7Bin Tang8Department of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyDepartment of Biomedical Engineering, Southern University of Science and TechnologyAbstract Background Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods We introduced a robust and simple approach of modifying titanium surfaces with polysaccharide complexes. Titanium samples were subjected to hydrothermal treatment to create a uniform porous structure on the surface, followed by coating with a bioinspired and self-assembly polydopamine layer. Strontium Eucommia Ulmoides Polysaccharide (EUP-Sr) complexes are then introduced to the polydopamine-coated porous titanium. Multiple morphological and physiochemical characterizations are employed for material evaluation, while cell proliferation and gene expression tests using macrophages, primary alveolar bone osteoblasts, and vascular endothelial cells are used to provide an overall insight into the functions of the product. The significances of statistical differences were analyzed using student’s t-test. Results Microscopic and spectrometric characterizations confirmed that the Ti surface formed a porous structure with an adequate amount of EUP-Sr loading. The attachment was attributed to hydrogen bonding between the ubiquitous glycosidic linkage of the polysaccharide complex and the ring structure of polydopamine, yet the loaded EUP-Sr complex can be gradually released, consequently benefiting the neighboring microenvironment. Cell experiments showed no cytotoxicity of the material, and the product showed promising anti-inflammation, osseointegration, and angiogenesis properties, which were further confirmed by in vivo evaluations. Conclusion We believe the EUP-Sr modified titanium implant is a promising candidate to be used in dental applications with notable osteoimmunomodulation and angiogenesis functions. And the novel technique proposed in this study would benefit the modification of metal/inorganic surfaces with polysaccharides for future research.https://doi.org/10.1186/s40824-023-00361-2Titanium dental implantBiological interfacesStrontium Eucommia Ulmoides polysaccharidesPolydopamineAnti-inflammationOsseointegration
spellingShingle Avery Rui Sun
Qili Sun
Yansong Wang
Liqiu Hu
Yutong Wu
Fenbo Ma
Jiayi Liu
Xiangchao Pang
Bin Tang
Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
Biomaterials Research
Titanium dental implant
Biological interfaces
Strontium Eucommia Ulmoides polysaccharides
Polydopamine
Anti-inflammation
Osseointegration
title Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
title_full Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
title_fullStr Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
title_full_unstemmed Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
title_short Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
title_sort surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation
topic Titanium dental implant
Biological interfaces
Strontium Eucommia Ulmoides polysaccharides
Polydopamine
Anti-inflammation
Osseointegration
url https://doi.org/10.1186/s40824-023-00361-2
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