Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis

Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat...

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Main Authors: Dede Saputra, Tati Nurhayati
Format: Article
Language:English
Published: Bina Nusantara University 2016-03-01
Series:ComTech
Subjects:
Online Access:https://journal.binus.ac.id/index.php/comtech/article/view/2201
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author Dede Saputra
Tati Nurhayati
author_facet Dede Saputra
Tati Nurhayati
author_sort Dede Saputra
collection DOAJ
description Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be identified by analyzing the content of dissolved total nitrogen (NTT) compared with nitrogen total ingredient (NTB) in order to get the value of total soluble nitrogen/total nitrogen material (NTT/NTB). The hydrolysis processes were carried out in 0,26% (w/v) papain, 60 οC for 3 hours. The result showed that the specific activity of papain enzyme was about 3.28 U/mg. Solubility of FPH by comparing NTT/NTB was about 0.29% (fish meat) and 0.40% (fish viscera). Proximate test of protein content of fish meat was 18.34 ± 0.04 (g/100 g); while viscera was about 0.95±0.04 (g/100 g). The result indicated that product waste of fish carp had potential as a major of source of FPH.
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spelling doaj.art-898a149e4d4e4d669d4fc87063059f6f2023-09-03T06:16:44ZengBina Nusantara UniversityComTech2087-12442476-907X2016-03-0171111810.21512/comtech.v7i1.22011613Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic HydrolysisDede Saputra0Tati Nurhayati1Bina Nusatara UniversityBogor Agricultural InstituteFish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be identified by analyzing the content of dissolved total nitrogen (NTT) compared with nitrogen total ingredient (NTB) in order to get the value of total soluble nitrogen/total nitrogen material (NTT/NTB). The hydrolysis processes were carried out in 0,26% (w/v) papain, 60 οC for 3 hours. The result showed that the specific activity of papain enzyme was about 3.28 U/mg. Solubility of FPH by comparing NTT/NTB was about 0.29% (fish meat) and 0.40% (fish viscera). Proximate test of protein content of fish meat was 18.34 ± 0.04 (g/100 g); while viscera was about 0.95±0.04 (g/100 g). The result indicated that product waste of fish carp had potential as a major of source of FPH.https://journal.binus.ac.id/index.php/comtech/article/view/2201enzymes, papain, protein, hydrolysates
spellingShingle Dede Saputra
Tati Nurhayati
Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
ComTech
enzymes, papain, protein, hydrolysates
title Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
title_full Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
title_fullStr Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
title_full_unstemmed Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
title_short Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis
title_sort production of fish hydrolysates protein from waste of fish carp cyprinus carpio by enzymatic hydrolysis
topic enzymes, papain, protein, hydrolysates
url https://journal.binus.ac.id/index.php/comtech/article/view/2201
work_keys_str_mv AT dedesaputra productionoffishhydrolysatesproteinfromwasteoffishcarpcyprinuscarpiobyenzymatichydrolysis
AT tatinurhayati productionoffishhydrolysatesproteinfromwasteoffishcarpcyprinuscarpiobyenzymatichydrolysis