Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study
ABSTRACT Objective: The aim of this in vitro study was to evaluate the antimicrobial effect of five types of non-industrialized and industrialized probiotics on biofilms formed around orthodontic mini-implants. The null hypothesis tested was: there is no difference in the antimicrobial effect betwe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dental Press Editora
2021-10-01
|
| Series: | Dental Press Journal of Orthodontics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512021000500302&tlng=en |
| _version_ | 1818775229240967168 |
|---|---|
| author | Ivana da Silva LEMOS Fernanda F. de Albuquerque JASSÉ Selly Sayuri SUZUKI Cristiane de Melo ALENCAR Denise Nami FUJII Joissi Ferrari ZANIBONI Hideo SUZUKI Aguinaldo Silva GARCEZ SEGUNDO |
| author_facet | Ivana da Silva LEMOS Fernanda F. de Albuquerque JASSÉ Selly Sayuri SUZUKI Cristiane de Melo ALENCAR Denise Nami FUJII Joissi Ferrari ZANIBONI Hideo SUZUKI Aguinaldo Silva GARCEZ SEGUNDO |
| author_sort | Ivana da Silva LEMOS |
| collection | DOAJ |
| description | ABSTRACT Objective: The aim of this in vitro study was to evaluate the antimicrobial effect of five types of non-industrialized and industrialized probiotics on biofilms formed around orthodontic mini-implants. The null hypothesis tested was: there is no difference in the antimicrobial effect between the five types of probiotics tested around orthodontic mini-implants. Methods: For the experiment, 120 mini-implants were immersed for seven days in Staphylococcus aureus solution for biofilm formation, and were subsequently plated in culture medium containing probiotics. The mini-implants were divided into six different groups, according to the probiotic used: G1)Lactobacillus casei; G2)Lactobacillus brevis; G3)Lactobacillus rhamnosus; G4) Lactobacillus from fermented milk Yakult®; G5) Lactobacillus from fermented milk Batavito® and G6) without use of probiotic, as negative control. Qualitative and quantitative analyses of all groups were performed using the CFU (colony forming unit) count. Results: The study showed that groups G4 and G6 did not present antimicrobial activity, in comparison to groups G1, G2, G3, and G5 (p< 0.05), which demonstrated antimicrobial activity. Conclusion: The non-commercial probiotic bacteria, Lactobacillus casei and Lactobacillus rhamnosus, as well as commercially available fermented milk Batavito® presented promising results in the reduction of colonization of mini-implants by S. aureus. Therefore, the null hypothesis was rejected. |
| first_indexed | 2024-12-18T10:53:43Z |
| format | Article |
| id | doaj.art-898b31a1461c44b79279c1db61ef6376 |
| institution | Directory Open Access Journal |
| issn | 2177-6709 |
| language | English |
| last_indexed | 2024-12-18T10:53:43Z |
| publishDate | 2021-10-01 |
| publisher | Dental Press Editora |
| record_format | Article |
| series | Dental Press Journal of Orthodontics |
| spelling | doaj.art-898b31a1461c44b79279c1db61ef63762022-12-21T21:10:23ZengDental Press EditoraDental Press Journal of Orthodontics2177-67092021-10-0126510.1590/2177-6709.26.5.e2119350.oarAntimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro studyIvana da Silva LEMOShttps://orcid.org/0000-0002-6107-9977Fernanda F. de Albuquerque JASSÉhttps://orcid.org/0000-0002-2293-2859Selly Sayuri SUZUKIhttps://orcid.org/0000-0001-7493-746XCristiane de Melo ALENCARhttps://orcid.org/0000-0002-0694-6863Denise Nami FUJIIhttps://orcid.org/0000-0002-6896-7539Joissi Ferrari ZANIBONIhttps://orcid.org/0000-0003-0172-9891Hideo SUZUKIhttps://orcid.org/0000-0002-7045-7095Aguinaldo Silva GARCEZ SEGUNDOhttps://orcid.org/0000-0003-2037-7211ABSTRACT Objective: The aim of this in vitro study was to evaluate the antimicrobial effect of five types of non-industrialized and industrialized probiotics on biofilms formed around orthodontic mini-implants. The null hypothesis tested was: there is no difference in the antimicrobial effect between the five types of probiotics tested around orthodontic mini-implants. Methods: For the experiment, 120 mini-implants were immersed for seven days in Staphylococcus aureus solution for biofilm formation, and were subsequently plated in culture medium containing probiotics. The mini-implants were divided into six different groups, according to the probiotic used: G1)Lactobacillus casei; G2)Lactobacillus brevis; G3)Lactobacillus rhamnosus; G4) Lactobacillus from fermented milk Yakult®; G5) Lactobacillus from fermented milk Batavito® and G6) without use of probiotic, as negative control. Qualitative and quantitative analyses of all groups were performed using the CFU (colony forming unit) count. Results: The study showed that groups G4 and G6 did not present antimicrobial activity, in comparison to groups G1, G2, G3, and G5 (p< 0.05), which demonstrated antimicrobial activity. Conclusion: The non-commercial probiotic bacteria, Lactobacillus casei and Lactobacillus rhamnosus, as well as commercially available fermented milk Batavito® presented promising results in the reduction of colonization of mini-implants by S. aureus. Therefore, the null hypothesis was rejected.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512021000500302&tlng=enMicrobiologyOral hygieneProbiotics |
| spellingShingle | Ivana da Silva LEMOS Fernanda F. de Albuquerque JASSÉ Selly Sayuri SUZUKI Cristiane de Melo ALENCAR Denise Nami FUJII Joissi Ferrari ZANIBONI Hideo SUZUKI Aguinaldo Silva GARCEZ SEGUNDO Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study Dental Press Journal of Orthodontics Microbiology Oral hygiene Probiotics |
| title | Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study |
| title_full | Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study |
| title_fullStr | Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study |
| title_full_unstemmed | Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study |
| title_short | Antimicrobial activity of probiotics against oral pathogens around orthodontic mini-implants: an in vitro study |
| title_sort | antimicrobial activity of probiotics against oral pathogens around orthodontic mini implants an in vitro study |
| topic | Microbiology Oral hygiene Probiotics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2176-94512021000500302&tlng=en |
| work_keys_str_mv | AT ivanadasilvalemos antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT fernandafdealbuquerquejasse antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT sellysayurisuzuki antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT cristianedemeloalencar antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT denisenamifujii antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT joissiferrarizaniboni antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT hideosuzuki antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy AT aguinaldosilvagarcezsegundo antimicrobialactivityofprobioticsagainstoralpathogensaroundorthodonticminiimplantsaninvitrostudy |