Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis
This study aimed to determine the prevalence, antibiotic resistance pattern, extended-spectrum, beta-lactamase production and biofilm forming ability of isolated E. coli strains from buffaloes mastitis milk. Out of 549 bacterial isolates from mastitis milk of buffaloes (n, animal level= 472) betwee...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-12-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/138397 |
| _version_ | 1797404521842868224 |
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| author | SARITA YADAV PARVINA DEVI ASHOK BOORA NISHU PRADEEP KUMAR AMANDEEP RAJESH KUMAR LOHCHAB ANIL KUMAR |
| author_facet | SARITA YADAV PARVINA DEVI ASHOK BOORA NISHU PRADEEP KUMAR AMANDEEP RAJESH KUMAR LOHCHAB ANIL KUMAR |
| author_sort | SARITA YADAV |
| collection | DOAJ |
| description |
This study aimed to determine the prevalence, antibiotic resistance pattern, extended-spectrum, beta-lactamase production and biofilm forming ability of isolated E. coli strains from buffaloes mastitis milk. Out of 549 bacterial isolates from mastitis milk of buffaloes (n, animal level= 472) between 2019 and 2022, a total of 43 E. coli strains were isolated with an overall prevalence of 9.11% at animal level. Prevalence of E coli was high in unorganised buffalo herd (11.36%) from villages of Farmer FIRST project (ICAR-FFP) compared with organised buffalo farms (6.73%). The highest resistance was against Penicillin 43 (100%) followed by Ceftriaxone 18 (41.86%), Amoxycillin/Sulbactam 8 (18.60%) and Enrofloxacin 7 (16.27%). Additionally, all were sensitive to gentamycin 43 (100%) followed by Cefoperazone/Sulbactam 34 (79.06%). Cephalosporins are frequently used antibiotics to treat bovine mastitis. However, their therapeutic effectiveness is being compromised by bacterial resistant to β-lactams. In present study, a total of 32 (6.78% %) extended-spectrum β-lactamase (ESBL) producing E coli were isolated from mastitic buffalo milk (n=43/472). In total, 17 (39.5%) isolates were biofilm producers by microtiter-plate method. There was statistically non-significant relationship between biofilm production and antibiotic resistance as well as between ESBL production and biofilm formation in E coli strains. Present study demonstrated a high occurrence of ESBL and biofilm producing E. coli in buffalo mastitis milk, implementing a significant challenge to treat mastitis in buffaloes, necessitates judicious use of antimicrobials and to explore potential therapeutic agents as substitutes for
antibiotics to treat bovine mastitis effectively.
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| first_indexed | 2024-03-09T02:56:12Z |
| format | Article |
| id | doaj.art-898d02509f4047c4be5dbf7e8b8e193c |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-09T02:56:12Z |
| publishDate | 2023-12-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-898d02509f4047c4be5dbf7e8b8e193c2023-12-05T05:36:02ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272023-12-01931210.56093/ijans.v93i12.138397Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitisSARITA YADAV0PARVINA DEVI1ASHOK BOORA2NISHU3PRADEEP KUMAR4AMANDEEP5RAJESH KUMAR LOHCHAB6ANIL KUMAR7ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 IndiaGuru Jambheshwar University of Science and Technology, Hisar, HaryanaGuru Jambheshwar University of Science and Technology, Hisar, Haryana. This study aimed to determine the prevalence, antibiotic resistance pattern, extended-spectrum, beta-lactamase production and biofilm forming ability of isolated E. coli strains from buffaloes mastitis milk. Out of 549 bacterial isolates from mastitis milk of buffaloes (n, animal level= 472) between 2019 and 2022, a total of 43 E. coli strains were isolated with an overall prevalence of 9.11% at animal level. Prevalence of E coli was high in unorganised buffalo herd (11.36%) from villages of Farmer FIRST project (ICAR-FFP) compared with organised buffalo farms (6.73%). The highest resistance was against Penicillin 43 (100%) followed by Ceftriaxone 18 (41.86%), Amoxycillin/Sulbactam 8 (18.60%) and Enrofloxacin 7 (16.27%). Additionally, all were sensitive to gentamycin 43 (100%) followed by Cefoperazone/Sulbactam 34 (79.06%). Cephalosporins are frequently used antibiotics to treat bovine mastitis. However, their therapeutic effectiveness is being compromised by bacterial resistant to β-lactams. In present study, a total of 32 (6.78% %) extended-spectrum β-lactamase (ESBL) producing E coli were isolated from mastitic buffalo milk (n=43/472). In total, 17 (39.5%) isolates were biofilm producers by microtiter-plate method. There was statistically non-significant relationship between biofilm production and antibiotic resistance as well as between ESBL production and biofilm formation in E coli strains. Present study demonstrated a high occurrence of ESBL and biofilm producing E. coli in buffalo mastitis milk, implementing a significant challenge to treat mastitis in buffaloes, necessitates judicious use of antimicrobials and to explore potential therapeutic agents as substitutes for antibiotics to treat bovine mastitis effectively. https://epubs.icar.org.in/index.php/IJAnS/article/view/138397Antimicrobial susceptibility test, Buffalo, Biofilm, E. coli, Farmer FIRST, Mastitis |
| spellingShingle | SARITA YADAV PARVINA DEVI ASHOK BOORA NISHU PRADEEP KUMAR AMANDEEP RAJESH KUMAR LOHCHAB ANIL KUMAR Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis Indian Journal of Animal Sciences Antimicrobial susceptibility test, Buffalo, Biofilm, E. coli, Farmer FIRST, Mastitis |
| title | Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis |
| title_full | Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis |
| title_fullStr | Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis |
| title_full_unstemmed | Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis |
| title_short | Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis |
| title_sort | prevalence extended spectrum β lactamase and biofilm production ability ofescherichia coli isolated from buffalo mastitis |
| topic | Antimicrobial susceptibility test, Buffalo, Biofilm, E. coli, Farmer FIRST, Mastitis |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/138397 |
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