From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy
Fourier Transform Infrared microspectroscopy (μFTIR) is a very useful method to analyze the biochemical properties of biological samples in situ. Many diseases affecting the central nervous system (CNS) have been studied using this method, to elucidate alterations in lipid oxidation or protein aggre...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/8/1099 |
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| author | Paula Sanchez-Molina Martin Kreuzer Núria Benseny-Cases Tony Valente Beatriz Almolda Berta González Bernardo Castellano Alex Perálvarez-Marín |
| author_facet | Paula Sanchez-Molina Martin Kreuzer Núria Benseny-Cases Tony Valente Beatriz Almolda Berta González Bernardo Castellano Alex Perálvarez-Marín |
| author_sort | Paula Sanchez-Molina |
| collection | DOAJ |
| description | Fourier Transform Infrared microspectroscopy (μFTIR) is a very useful method to analyze the biochemical properties of biological samples in situ. Many diseases affecting the central nervous system (CNS) have been studied using this method, to elucidate alterations in lipid oxidation or protein aggregation, among others. In this work, we describe in detail the characteristics between grey matter (GM) and white matter (WM) areas of the human brain by μFTIR, and we compare them with the mouse brain (strain C57BL/6), the most used animal model in neurological disorders. Our results show a clear different infrared profile between brain areas in the lipid region of both species. After applying a second derivative in the data, we established a 1.5 threshold value for the lipid/protein ratio to discriminate between GM and WM areas in non-pathological conditions. Furthermore, we demonstrated intrinsic differences of lipids and proteins by cerebral area. Lipids from GM present higher C=CH, C=O and CH<sub>3</sub> functional groups compared to WM in humans and mice. Regarding proteins, GM present lower Amide II amounts and higher intramolecular β-sheet structure amounts with respect to WM in both species. However, the presence of intermolecular β-sheet structures, which is related to β-aggregation, was only observed in the GM of some human individuals. The present study defines the relevant biochemical properties of non-pathological human and mouse brains by μFTIR as a benchmark for future studies involving CNS pathological samples. |
| first_indexed | 2024-03-10T18:15:06Z |
| format | Article |
| id | doaj.art-89935ae665ee43ccb19807083667f6ef |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T18:15:06Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-89935ae665ee43ccb19807083667f6ef2023-11-20T07:48:48ZengMDPI AGBiomolecules2218-273X2020-07-01108109910.3390/biom10081099From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared MicrospectroscopyPaula Sanchez-Molina0Martin Kreuzer1Núria Benseny-Cases2Tony Valente3Beatriz Almolda4Berta González5Bernardo Castellano6Alex Perálvarez-Marín7Institute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainALBA Synchrotron Light Source, Carrer de la Llum 2-26, 08290 Cerdanyola del Vallès, Barcelona, Catalonia, SpainALBA Synchrotron Light Source, Carrer de la Llum 2-26, 08290 Cerdanyola del Vallès, Barcelona, Catalonia, SpainInstitute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainInstitute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainInstitute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainInstitute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainInstitute of Neurosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainFourier Transform Infrared microspectroscopy (μFTIR) is a very useful method to analyze the biochemical properties of biological samples in situ. Many diseases affecting the central nervous system (CNS) have been studied using this method, to elucidate alterations in lipid oxidation or protein aggregation, among others. In this work, we describe in detail the characteristics between grey matter (GM) and white matter (WM) areas of the human brain by μFTIR, and we compare them with the mouse brain (strain C57BL/6), the most used animal model in neurological disorders. Our results show a clear different infrared profile between brain areas in the lipid region of both species. After applying a second derivative in the data, we established a 1.5 threshold value for the lipid/protein ratio to discriminate between GM and WM areas in non-pathological conditions. Furthermore, we demonstrated intrinsic differences of lipids and proteins by cerebral area. Lipids from GM present higher C=CH, C=O and CH<sub>3</sub> functional groups compared to WM in humans and mice. Regarding proteins, GM present lower Amide II amounts and higher intramolecular β-sheet structure amounts with respect to WM in both species. However, the presence of intermolecular β-sheet structures, which is related to β-aggregation, was only observed in the GM of some human individuals. The present study defines the relevant biochemical properties of non-pathological human and mouse brains by μFTIR as a benchmark for future studies involving CNS pathological samples.https://www.mdpi.com/2218-273X/10/8/1099infrared spectroscopygrey matterwhite matterlipid oxidationprotein structurecentral nervous system |
| spellingShingle | Paula Sanchez-Molina Martin Kreuzer Núria Benseny-Cases Tony Valente Beatriz Almolda Berta González Bernardo Castellano Alex Perálvarez-Marín From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy Biomolecules infrared spectroscopy grey matter white matter lipid oxidation protein structure central nervous system |
| title | From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy |
| title_full | From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy |
| title_fullStr | From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy |
| title_full_unstemmed | From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy |
| title_short | From Mouse to Human: Comparative Analysis between Grey and White Matter by Synchrotron-Fourier Transformed Infrared Microspectroscopy |
| title_sort | from mouse to human comparative analysis between grey and white matter by synchrotron fourier transformed infrared microspectroscopy |
| topic | infrared spectroscopy grey matter white matter lipid oxidation protein structure central nervous system |
| url | https://www.mdpi.com/2218-273X/10/8/1099 |
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