Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures
Summary: Neurotrophic factors and their signaling cascades play important roles in synaptic growth, which can be investigated in cultured primary neurons to better control the concentrations and timing of neurotrophic factor treatment. Here, we provide a protocol detailing the preparation of culture...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721008182 |
| _version_ | 1818646543448670208 |
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| author | Guiqin He Xin-Yue Wang Zhengping Jia Zikai Zhou |
| author_facet | Guiqin He Xin-Yue Wang Zhengping Jia Zikai Zhou |
| author_sort | Guiqin He |
| collection | DOAJ |
| description | Summary: Neurotrophic factors and their signaling cascades play important roles in synaptic growth, which can be investigated in cultured primary neurons to better control the concentrations and timing of neurotrophic factor treatment. Here, we provide a protocol detailing the preparation of cultured primary mouse neurons and the neurotrophic factor treatment. We then describe electrophysiological recording of synaptic transmission, immunocytochemistry of AMPA receptor expression, and imaging analysis of dendritic spines. This platform enables characterization of synaptic growth at functional and morphological levels.For complete details on the use and execution of this profile, please refer to Zhou et al. (2021). |
| first_indexed | 2024-12-17T00:48:19Z |
| format | Article |
| id | doaj.art-89964b05b07b4e7bbe31ec22e15ee876 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T00:48:19Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-89964b05b07b4e7bbe31ec22e15ee8762022-12-21T22:09:50ZengElsevierSTAR Protocols2666-16672022-03-0131101112Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal culturesGuiqin He0Xin-Yue Wang1Zhengping Jia2Zikai Zhou3Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, Guangdong 528400, ChinaShanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, ChinaNeurosciences & Mental Health, The Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada; Department of Physiology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, Toronto, ON M5S 1A8, Canada; Corresponding authorZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, Guangdong 528400, China; Corresponding authorSummary: Neurotrophic factors and their signaling cascades play important roles in synaptic growth, which can be investigated in cultured primary neurons to better control the concentrations and timing of neurotrophic factor treatment. Here, we provide a protocol detailing the preparation of cultured primary mouse neurons and the neurotrophic factor treatment. We then describe electrophysiological recording of synaptic transmission, immunocytochemistry of AMPA receptor expression, and imaging analysis of dendritic spines. This platform enables characterization of synaptic growth at functional and morphological levels.For complete details on the use and execution of this profile, please refer to Zhou et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721008182Cell BiologyCell cultureCell isolationMicroscopyMolecular BiologyNeuroscience |
| spellingShingle | Guiqin He Xin-Yue Wang Zhengping Jia Zikai Zhou Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures STAR Protocols Cell Biology Cell culture Cell isolation Microscopy Molecular Biology Neuroscience |
| title | Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures |
| title_full | Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures |
| title_fullStr | Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures |
| title_full_unstemmed | Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures |
| title_short | Characterizing neurotrophic factor-induced synaptic growth in primary mouse neuronal cultures |
| title_sort | characterizing neurotrophic factor induced synaptic growth in primary mouse neuronal cultures |
| topic | Cell Biology Cell culture Cell isolation Microscopy Molecular Biology Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721008182 |
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