Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i>
Black spot needle blight is a serious conifer disease of <i>Pinus sylvestris</i> var. <i>mongolica</i> occurring in Northeast China, which is usually caused by the plant pathogenic fungus <i>Pestalotiopsis neglecta</i>. From the diseased pine needles collected in...
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MDPI AG
2023-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/5/564 |
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| author | Jing Yang Shuren Wang Yundi Zhang Yunze Chen Heying Zhou Guocai Zhang |
| author_facet | Jing Yang Shuren Wang Yundi Zhang Yunze Chen Heying Zhou Guocai Zhang |
| author_sort | Jing Yang |
| collection | DOAJ |
| description | Black spot needle blight is a serious conifer disease of <i>Pinus sylvestris</i> var. <i>mongolica</i> occurring in Northeast China, which is usually caused by the plant pathogenic fungus <i>Pestalotiopsis neglecta</i>. From the diseased pine needles collected in Honghuaerji, the <i>P. neglecta</i> strain YJ-3 was isolated and identified as the phytopathogen, and its culture characteristics were studied. Then, we generated a highly contiguous 48.36-Mbp genome assembly (N50 = 6.62 Mbp) of the <i>P. neglecta</i> strain YJ-3 by combining the PacBio RS II Single Molecule Real Time (SMRT) and Illumina HiSeq X Ten sequencing platforms. The results showed that a total of 13,667 protein-coding genes were predicted and annotated using multiple bioinformatics databases. The genome assembly and annotation resource reported here will be useful for the study of fungal infection mechanisms and pathogen–host interaction. |
| first_indexed | 2024-03-11T03:35:36Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T03:35:36Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-899cbe384d8849c1a0c82aeb022b4dca2023-11-18T02:02:24ZengMDPI AGJournal of Fungi2309-608X2023-05-019556410.3390/jof9050564Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i>Jing Yang0Shuren Wang1Yundi Zhang2Yunze Chen3Heying Zhou4Guocai Zhang5College of Forestry, Guizhou University, Huaxi District, Guiyang 550025, ChinaHeilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, ChinaHeilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, ChinaSchool of Biological Sciences, Guizhou Education University, Wudang District, Guiyang 550018, ChinaCollege of Forestry, Guizhou University, Huaxi District, Guiyang 550025, ChinaHeilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, ChinaBlack spot needle blight is a serious conifer disease of <i>Pinus sylvestris</i> var. <i>mongolica</i> occurring in Northeast China, which is usually caused by the plant pathogenic fungus <i>Pestalotiopsis neglecta</i>. From the diseased pine needles collected in Honghuaerji, the <i>P. neglecta</i> strain YJ-3 was isolated and identified as the phytopathogen, and its culture characteristics were studied. Then, we generated a highly contiguous 48.36-Mbp genome assembly (N50 = 6.62 Mbp) of the <i>P. neglecta</i> strain YJ-3 by combining the PacBio RS II Single Molecule Real Time (SMRT) and Illumina HiSeq X Ten sequencing platforms. The results showed that a total of 13,667 protein-coding genes were predicted and annotated using multiple bioinformatics databases. The genome assembly and annotation resource reported here will be useful for the study of fungal infection mechanisms and pathogen–host interaction.https://www.mdpi.com/2309-608X/9/5/564fungal geneticsgene annotation<i>Pestalotiopsis neglecta</i>phytopathogenic fungus |
| spellingShingle | Jing Yang Shuren Wang Yundi Zhang Yunze Chen Heying Zhou Guocai Zhang Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> Journal of Fungi fungal genetics gene annotation <i>Pestalotiopsis neglecta</i> phytopathogenic fungus |
| title | Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> |
| title_full | Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> |
| title_fullStr | Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> |
| title_full_unstemmed | Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> |
| title_short | Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i> |
| title_sort | identification culture characteristics and whole genome analysis of i pestalotiopsis neglecta i causing black spot blight of i pinus sylvestris i var i mongolica i |
| topic | fungal genetics gene annotation <i>Pestalotiopsis neglecta</i> phytopathogenic fungus |
| url | https://www.mdpi.com/2309-608X/9/5/564 |
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