Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis
Photodynamic Therapy (PDT) holds a great promise for cancer patients, however, due to the hypoxic characteristics of most solid tumors and the limited penetration depth of light in tissues, the extensive clinical application of PDT is limited. Herein, we report microwave induced copper-cysteamine (C...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-06-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22005199 |
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| author | Hui Zhou Zhongtao Liu Zijian Zhang Nil Kanatha Pandey Eric Amador William Nguyen Lalit Chudal Li Xiong Wei Chen Yu Wen |
| author_facet | Hui Zhou Zhongtao Liu Zijian Zhang Nil Kanatha Pandey Eric Amador William Nguyen Lalit Chudal Li Xiong Wei Chen Yu Wen |
| author_sort | Hui Zhou |
| collection | DOAJ |
| description | Photodynamic Therapy (PDT) holds a great promise for cancer patients, however, due to the hypoxic characteristics of most solid tumors and the limited penetration depth of light in tissues, the extensive clinical application of PDT is limited. Herein, we report microwave induced copper-cysteamine (Cu-Cy) nanoparticles-based PDT as a promising cancer treatment to overcome cancer resistance in combination with ferroptosis. The treatment efficiency of Cu-Cy-mediated microwave dynamic therapy (MWDT) tested on HCT15 colorectal cancer (CRC) cells via cell titer-blue cell viability assay and live/dead assay reveal that Cu-Cy upon MW irradiation can effectively destroy HCT15 CRC cells with average IC-50 values of 20 μg/mL. The cytotoxicity of Cu-Cy to tumor cells after MW stimulation can be alleviated by ferroptosis inhibitor. Furthermore, Cu-Cy mediated MWDT could deplete glutathione peroxide 4 (GPX4) and enhance lipid peroxides (LPO) and malondialdehyde (MDA). Our findings demonstrate that MW-activated Cu-Cy killed CRC cells by inducing ferroptosis. The superior in vivo antitumor efficacy of the Cu-Cy was corroborated by a HCT15 tumor-bearing mice model. Immunohistochemical experiments showed that the GPX4 expression level in Cu-Cy + MW group was significantly lower than that in other groups. Overall, these findings demonstrate that Cu-Cy nanoparticles have a safe and promising clinical application prospect in MWDT for deep-seated tumors and effectively inhibit tumor cell proliferation by inducing ferroptosis, which provides a potential solution for cancer resistance. |
| first_indexed | 2024-04-09T23:18:39Z |
| format | Article |
| id | doaj.art-899cf60ed5914029bd7e11e91ba79240 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-09T23:18:39Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-899cf60ed5914029bd7e11e91ba792402023-03-22T04:37:34ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-06-0124322330Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosisHui Zhou0Zhongtao Liu1Zijian Zhang2Nil Kanatha Pandey3Eric Amador4William Nguyen5Lalit Chudal6Li Xiong7Wei Chen8Yu Wen9Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, ChinaDepartment of General Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, ChinaDepartment of General Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, ChinaDepartment of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USADepartment of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USADepartment of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USADepartment of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USADepartment of General Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, China; Correponding authorDepartment of Physics, The University of Texas at Arlington, Arlington, TX, 76019-0059, USA; Corresponding author.Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha, 410011, China; Corresponding author.Photodynamic Therapy (PDT) holds a great promise for cancer patients, however, due to the hypoxic characteristics of most solid tumors and the limited penetration depth of light in tissues, the extensive clinical application of PDT is limited. Herein, we report microwave induced copper-cysteamine (Cu-Cy) nanoparticles-based PDT as a promising cancer treatment to overcome cancer resistance in combination with ferroptosis. The treatment efficiency of Cu-Cy-mediated microwave dynamic therapy (MWDT) tested on HCT15 colorectal cancer (CRC) cells via cell titer-blue cell viability assay and live/dead assay reveal that Cu-Cy upon MW irradiation can effectively destroy HCT15 CRC cells with average IC-50 values of 20 μg/mL. The cytotoxicity of Cu-Cy to tumor cells after MW stimulation can be alleviated by ferroptosis inhibitor. Furthermore, Cu-Cy mediated MWDT could deplete glutathione peroxide 4 (GPX4) and enhance lipid peroxides (LPO) and malondialdehyde (MDA). Our findings demonstrate that MW-activated Cu-Cy killed CRC cells by inducing ferroptosis. The superior in vivo antitumor efficacy of the Cu-Cy was corroborated by a HCT15 tumor-bearing mice model. Immunohistochemical experiments showed that the GPX4 expression level in Cu-Cy + MW group was significantly lower than that in other groups. Overall, these findings demonstrate that Cu-Cy nanoparticles have a safe and promising clinical application prospect in MWDT for deep-seated tumors and effectively inhibit tumor cell proliferation by inducing ferroptosis, which provides a potential solution for cancer resistance.http://www.sciencedirect.com/science/article/pii/S2452199X22005199Cu-CyMicrowavePDTCell deathColorectal cancerFerroptosis |
| spellingShingle | Hui Zhou Zhongtao Liu Zijian Zhang Nil Kanatha Pandey Eric Amador William Nguyen Lalit Chudal Li Xiong Wei Chen Yu Wen Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis Bioactive Materials Cu-Cy Microwave PDT Cell death Colorectal cancer Ferroptosis |
| title | Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| title_full | Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| title_fullStr | Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| title_full_unstemmed | Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| title_short | Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| title_sort | copper cysteamine nanoparticle mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis |
| topic | Cu-Cy Microwave PDT Cell death Colorectal cancer Ferroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22005199 |
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