Strand Displacement Activity of PrimPol
Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn<sup>2+</sup> ions and accessory replicative prote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9027 |
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| author | Elizaveta O. Boldinova Ekaterina A. Belousova Diana I. Gagarinskaya Ekaterina A. Maltseva Svetlana N. Khodyreva Olga I. Lavrik Alena V. Makarova |
| author_facet | Elizaveta O. Boldinova Ekaterina A. Belousova Diana I. Gagarinskaya Ekaterina A. Maltseva Svetlana N. Khodyreva Olga I. Lavrik Alena V. Makarova |
| author_sort | Elizaveta O. Boldinova |
| collection | DOAJ |
| description | Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn<sup>2+</sup> ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro. |
| first_indexed | 2024-03-10T14:30:28Z |
| format | Article |
| id | doaj.art-899d4a6ed2f04a23b702553083f99fd4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:30:28Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-899d4a6ed2f04a23b702553083f99fd42023-11-20T22:38:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012123902710.3390/ijms21239027Strand Displacement Activity of PrimPolElizaveta O. Boldinova0Ekaterina A. Belousova1Diana I. Gagarinskaya2Ekaterina A. Maltseva3Svetlana N. Khodyreva4Olga I. Lavrik5Alena V. Makarova6Institute of Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov sq. 2, 123182 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090 Novosibirsk, RussiaInstitute of Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov sq. 2, 123182 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090 Novosibirsk, RussiaInstitute of Molecular Genetics, National Research Center “Kurchatov Institute”, Kurchatov sq. 2, 123182 Moscow, RussiaHuman PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn<sup>2+</sup> ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro.https://www.mdpi.com/1422-0067/21/23/9027PrimPolstrand displacementRPAPolDIP2FEN1 |
| spellingShingle | Elizaveta O. Boldinova Ekaterina A. Belousova Diana I. Gagarinskaya Ekaterina A. Maltseva Svetlana N. Khodyreva Olga I. Lavrik Alena V. Makarova Strand Displacement Activity of PrimPol International Journal of Molecular Sciences PrimPol strand displacement RPA PolDIP2 FEN1 |
| title | Strand Displacement Activity of PrimPol |
| title_full | Strand Displacement Activity of PrimPol |
| title_fullStr | Strand Displacement Activity of PrimPol |
| title_full_unstemmed | Strand Displacement Activity of PrimPol |
| title_short | Strand Displacement Activity of PrimPol |
| title_sort | strand displacement activity of primpol |
| topic | PrimPol strand displacement RPA PolDIP2 FEN1 |
| url | https://www.mdpi.com/1422-0067/21/23/9027 |
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