Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols
Background and Objectives: Skeletal muscle mass depends on the balance between synthesis and degradation of muscle protein, which changes with aging and disease. The aim of the present reserch was to examine the effects of two exercise training protocols on alterations of some genes involved in path...
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2019-08-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
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| Online Access: | http://journal.muq.ac.ir/article-1-2495-en.html |
| _version_ | 1818911397959958528 |
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| author | Majid Gholipour Soheyla Mazaheri Mohammad Reza Asad |
| author_facet | Majid Gholipour Soheyla Mazaheri Mohammad Reza Asad |
| author_sort | Majid Gholipour |
| collection | DOAJ |
| description | Background and Objectives: Skeletal muscle mass depends on the balance between synthesis and degradation of muscle protein, which changes with aging and disease. The aim of the present reserch was to examine the effects of two exercise training protocols on alterations of some genes involved in pathways of protein synthesis and degradation in order to achieve a more effective training program in case of pathological conditions, aging process, and improvement of athletic performance.
Methods: In this experimental study, 8 week old Wistar rats, were randomly divided into three groups of resistance training, high-intensity interval training, and control. To examine gene expression, 48 hours after the last exercise session, the Soleus muscles were dissected and stored at -80C. Between-group differences, were analysed using parametric and non-parametric tests at the significance level of α˂0.05.
Results: Compared to the control group, the increased expression of AKT and Rps6kb1 genes, was significant in both training groups (especially resistance group). Moreover, the gene expression related to the protein degradation signaling pathway, (MuRF1 and Map1LC3a), were suppressed by both training protocols, especially high-intensity interval training group and were confirmed by Western blotting analysis.
Conclusion: Given the fact that the protein synthesis-related gene expression was increased by resistance training and protein degradation-related gene expression was suppressed by high-intensity interval training, the practical application of the two protocols can be considered simultaneously in order to reduce muscle atrophy in pathological conditions, aging, as well as to increase muscle strength to improve athletic performance. |
| first_indexed | 2024-12-19T22:58:04Z |
| format | Article |
| id | doaj.art-899d5699b3004c94b3305be2a68bfa99 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-19T22:58:04Z |
| publishDate | 2019-08-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-899d5699b3004c94b3305be2a68bfa992022-12-21T20:02:35ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752019-08-011362737Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training ProtocolsMajid Gholipour0Soheyla Mazaheri1Mohammad Reza Asad2 Department of Physical Education, Sharif University of Technology Department of Sport Physiology, Karaj Payame Noor University Department of Physical Education & Sport Sciences, School of Education & Psychology, Payame Noor University Background and Objectives: Skeletal muscle mass depends on the balance between synthesis and degradation of muscle protein, which changes with aging and disease. The aim of the present reserch was to examine the effects of two exercise training protocols on alterations of some genes involved in pathways of protein synthesis and degradation in order to achieve a more effective training program in case of pathological conditions, aging process, and improvement of athletic performance. Methods: In this experimental study, 8 week old Wistar rats, were randomly divided into three groups of resistance training, high-intensity interval training, and control. To examine gene expression, 48 hours after the last exercise session, the Soleus muscles were dissected and stored at -80C. Between-group differences, were analysed using parametric and non-parametric tests at the significance level of α˂0.05. Results: Compared to the control group, the increased expression of AKT and Rps6kb1 genes, was significant in both training groups (especially resistance group). Moreover, the gene expression related to the protein degradation signaling pathway, (MuRF1 and Map1LC3a), were suppressed by both training protocols, especially high-intensity interval training group and were confirmed by Western blotting analysis. Conclusion: Given the fact that the protein synthesis-related gene expression was increased by resistance training and protein degradation-related gene expression was suppressed by high-intensity interval training, the practical application of the two protocols can be considered simultaneously in order to reduce muscle atrophy in pathological conditions, aging, as well as to increase muscle strength to improve athletic performance.http://journal.muq.ac.ir/article-1-2495-en.htmlexercise traininggene expressionwistar ratsskeletal |
| spellingShingle | Majid Gholipour Soheyla Mazaheri Mohammad Reza Asad Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum exercise training gene expression wistar rats skeletal |
| title | Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols |
| title_full | Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols |
| title_fullStr | Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols |
| title_full_unstemmed | Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols |
| title_short | Comparison of the Alterations of Gene Expression Related to Signaling Pathways of Synthesis and Degradation of Skeletal Muscle Protein Induced by Two Exercise Training Protocols |
| title_sort | comparison of the alterations of gene expression related to signaling pathways of synthesis and degradation of skeletal muscle protein induced by two exercise training protocols |
| topic | exercise training gene expression wistar rats skeletal |
| url | http://journal.muq.ac.ir/article-1-2495-en.html |
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