Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato
Core Ideas First study of PpEIN3 by transgenic experiments to verify its function in the maturity process PpEIN3 is a positive regulator of ethylene signal transduction pathway to promote fruits ripening Ethylene is one of the most important phytohormone in plants and plays a critical role during gr...
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-11-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.3835/plantgenome2018.11.0089 |
| _version_ | 1831552182642540544 |
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| author | Zhang Yanping Huang Yuqing Wang Chen Mu Qian Jiu Songtao Zhu Xudong Zheng Ting Zhang Kekun Jia Haifeng Pervaiz Tariq Fang Jinggui |
| author_facet | Zhang Yanping Huang Yuqing Wang Chen Mu Qian Jiu Songtao Zhu Xudong Zheng Ting Zhang Kekun Jia Haifeng Pervaiz Tariq Fang Jinggui |
| author_sort | Zhang Yanping |
| collection | DOAJ |
| description | Core Ideas First study of PpEIN3 by transgenic experiments to verify its function in the maturity process PpEIN3 is a positive regulator of ethylene signal transduction pathway to promote fruits ripening Ethylene is one of the most important phytohormone in plants and plays a critical role during growth, development, maturity, and aging. The framework of the ethylene signaling pathway is well reported. Nevertheless, studies on Ethylene Insensitive 3 (EIN3), the downstream regulator of the ethylene signaling pathway, need to be investigated, especially in peach [Prunus persica (L.) Batsch]. In this study, we cloned PpEIN3 from peach and characterized it in tomato (Solanum lycopersicum L.). Our results depicted that the open‐reading frame of PpEIN3 was 1875 bp, encoding a protein with 624 amino acid residues that contained a conserved EIN3 domain, a highly conserved N‐terminal region, and seven DNA‐binding sites. PpEIN3 showed very close association with homologous EIN genes from apple (Malus domestica Borkh.) and grapevine (Vitis vinifera L.). All investigated EIN proteins shared similar domains and structures. The PpEIN3 promoter possessed several motifs related to hormones that affect fruit development and ripening. Spatial–temporal expression analysis revealed that PpEIN3 was expressed at high levels in the late stage of fruit development vs. the early stage. In transgenic tomato, PpEIIN3 showed overexpression and the key ethylene biosynthesis genes SlACO1, SlACS1, and SlSAMS1 were upregulated and promoted early maturation in fruit. By contrast, PpEIIN3 silencing delayed ripening and reduced SlEIN3 expression in tomato. The results confirmed that PpEIN3 is a positive regulator of the ethylene signal transduction pathway, which promoted fruit ripening. Our findings provide valuable insight to the roles in ethylene signal components in the modulation of peach fruit ripening. |
| first_indexed | 2024-12-17T03:22:25Z |
| format | Article |
| id | doaj.art-899d5f09550b472abf946930fa97b0ca |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-12-17T03:22:25Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-899d5f09550b472abf946930fa97b0ca2022-12-21T22:05:29ZengWileyThe Plant Genome1940-33722019-11-01123n/an/a10.3835/plantgenome2018.11.0089Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in TomatoZhang Yanping0Huang Yuqing1Wang Chen2Mu Qian3Jiu Songtao4Zhu Xudong5Zheng Ting6Zhang Kekun7Jia Haifeng8Pervaiz Tariq9Fang Jinggui10College of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCollege of HorticultureNanjing Agricultural Univ.Nanjing210095ChinaCore Ideas First study of PpEIN3 by transgenic experiments to verify its function in the maturity process PpEIN3 is a positive regulator of ethylene signal transduction pathway to promote fruits ripening Ethylene is one of the most important phytohormone in plants and plays a critical role during growth, development, maturity, and aging. The framework of the ethylene signaling pathway is well reported. Nevertheless, studies on Ethylene Insensitive 3 (EIN3), the downstream regulator of the ethylene signaling pathway, need to be investigated, especially in peach [Prunus persica (L.) Batsch]. In this study, we cloned PpEIN3 from peach and characterized it in tomato (Solanum lycopersicum L.). Our results depicted that the open‐reading frame of PpEIN3 was 1875 bp, encoding a protein with 624 amino acid residues that contained a conserved EIN3 domain, a highly conserved N‐terminal region, and seven DNA‐binding sites. PpEIN3 showed very close association with homologous EIN genes from apple (Malus domestica Borkh.) and grapevine (Vitis vinifera L.). All investigated EIN proteins shared similar domains and structures. The PpEIN3 promoter possessed several motifs related to hormones that affect fruit development and ripening. Spatial–temporal expression analysis revealed that PpEIN3 was expressed at high levels in the late stage of fruit development vs. the early stage. In transgenic tomato, PpEIIN3 showed overexpression and the key ethylene biosynthesis genes SlACO1, SlACS1, and SlSAMS1 were upregulated and promoted early maturation in fruit. By contrast, PpEIIN3 silencing delayed ripening and reduced SlEIN3 expression in tomato. The results confirmed that PpEIN3 is a positive regulator of the ethylene signal transduction pathway, which promoted fruit ripening. Our findings provide valuable insight to the roles in ethylene signal components in the modulation of peach fruit ripening.https://doi.org/10.3835/plantgenome2018.11.0089 |
| spellingShingle | Zhang Yanping Huang Yuqing Wang Chen Mu Qian Jiu Songtao Zhu Xudong Zheng Ting Zhang Kekun Jia Haifeng Pervaiz Tariq Fang Jinggui Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato The Plant Genome |
| title | Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato |
| title_full | Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato |
| title_fullStr | Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato |
| title_full_unstemmed | Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato |
| title_short | Characterization and Identification of PpEIN3 during the Modulation of Fruit Ripening Process by Ectopic Expressions in Tomato |
| title_sort | characterization and identification of ppein3 during the modulation of fruit ripening process by ectopic expressions in tomato |
| url | https://doi.org/10.3835/plantgenome2018.11.0089 |
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