Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells
The phenolic composition of Syrah and Chardonnay grape pomaces was studied to assess their antioxidant and prooxidant properties. Polyphenols were extracted by a "green" hydroalcoholic solvent (ethanol/water 1:1 <i>v</i>/<i>v</i>), and a detailed chemical and electr...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/80 |
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| author | Ylenia Spissu Katarzyna Angelika Gil Antonio Dore Giulia Sanna Giuseppe Palmieri Andrea Sanna Maurizio Cossu Feten Belhadj Boutheina Gharbi Maria Barbara Pinna Antonio Barberis Carlo Ignazio Giovanni Tuberoso Guy D’hallewin |
| author_facet | Ylenia Spissu Katarzyna Angelika Gil Antonio Dore Giulia Sanna Giuseppe Palmieri Andrea Sanna Maurizio Cossu Feten Belhadj Boutheina Gharbi Maria Barbara Pinna Antonio Barberis Carlo Ignazio Giovanni Tuberoso Guy D’hallewin |
| author_sort | Ylenia Spissu |
| collection | DOAJ |
| description | The phenolic composition of Syrah and Chardonnay grape pomaces was studied to assess their antioxidant and prooxidant properties. Polyphenols were extracted by a "green" hydroalcoholic solvent (ethanol/water 1:1 <i>v</i>/<i>v</i>), and a detailed chemical and electrochemical characterization of the phenolic compounds was performed. The antioxidant and prooxidant capacity of the pomace was first studied by cyclic voltammetry (CV) and other reference analytical assays, then with biological tests on B16F10 metastatic melanoma cancer cells. Electrochemical data showed that, when a +0.5 V potential was applied, a low to moderate antioxidant capacity was observed. MTT test showed an increasing viability of melanoma cells, after treatments at low concentration (up to 100 μg/mL) and for a short time (6 h), but when cells were treated with higher doses of extract (≥250 μg/mL for 12/24 h), their viability decreased from 25 to 50% vs. control, depending on treatment time, dose, and extract origin. A stronger prooxidant activity resulted when 250 μg/mL of extract was combined with non-toxic doses of H<sub>2</sub>O<sub>2</sub>; this activity was correlated with the presence of copper in the extracts. This study shows the potential of winemaking by-products and suggests the opportunity to exploit them for the production of cosmeceuticals, or for combined therapies with approved anticancer drugs. |
| first_indexed | 2024-03-09T13:47:18Z |
| format | Article |
| id | doaj.art-89a197ebbe6142bcab4c0f6221a007db |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:47:18Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-89a197ebbe6142bcab4c0f6221a007db2023-11-30T20:58:02ZengMDPI AGAntioxidants2076-39212022-12-011218010.3390/antiox12010080Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer CellsYlenia Spissu0Katarzyna Angelika Gil1Antonio Dore2Giulia Sanna3Giuseppe Palmieri4Andrea Sanna5Maurizio Cossu6Feten Belhadj7Boutheina Gharbi8Maria Barbara Pinna9Antonio Barberis10Carlo Ignazio Giovanni Tuberoso11Guy D’hallewin12Institute of Sciences of Food Production (ISPA), 07100 Sassari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, ItalyInstitute of Sciences of Food Production (ISPA), 07100 Sassari, ItalyInstitute of Sciences of Food Production (ISPA), 07100 Sassari, ItalyInstitute for Genetic and Biomedical Research (IRGB), 07100 Sassari, ItalyIZS Sardegna SC Chimica, 07100 Sassari, ItalyIZS Sardegna SC Chimica, 07100 Sassari, ItalyUTICA-Union Tunisienne de l’Industrie du Commerce et de l’Artisanat, Rue Ferjani Bel Haj Ammar, Cité Khadhra, Tunis 1003, TunisiaUTICA-Union Tunisienne de l’Industrie du Commerce et de l’Artisanat, Rue Ferjani Bel Haj Ammar, Cité Khadhra, Tunis 1003, TunisiaLaboratory of Research and Analysis Cantina Argiolas, 09040 Serdiana, ItalyInstitute of Sciences of Food Production (ISPA), 07100 Sassari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, ItalyInstitute of Sciences of Food Production (ISPA), 07100 Sassari, ItalyThe phenolic composition of Syrah and Chardonnay grape pomaces was studied to assess their antioxidant and prooxidant properties. Polyphenols were extracted by a "green" hydroalcoholic solvent (ethanol/water 1:1 <i>v</i>/<i>v</i>), and a detailed chemical and electrochemical characterization of the phenolic compounds was performed. The antioxidant and prooxidant capacity of the pomace was first studied by cyclic voltammetry (CV) and other reference analytical assays, then with biological tests on B16F10 metastatic melanoma cancer cells. Electrochemical data showed that, when a +0.5 V potential was applied, a low to moderate antioxidant capacity was observed. MTT test showed an increasing viability of melanoma cells, after treatments at low concentration (up to 100 μg/mL) and for a short time (6 h), but when cells were treated with higher doses of extract (≥250 μg/mL for 12/24 h), their viability decreased from 25 to 50% vs. control, depending on treatment time, dose, and extract origin. A stronger prooxidant activity resulted when 250 μg/mL of extract was combined with non-toxic doses of H<sub>2</sub>O<sub>2</sub>; this activity was correlated with the presence of copper in the extracts. This study shows the potential of winemaking by-products and suggests the opportunity to exploit them for the production of cosmeceuticals, or for combined therapies with approved anticancer drugs.https://www.mdpi.com/2076-3921/12/1/80polyphenolsgrapevine pomaceantioxidantscyclic voltammetryprooxidant activitycopper |
| spellingShingle | Ylenia Spissu Katarzyna Angelika Gil Antonio Dore Giulia Sanna Giuseppe Palmieri Andrea Sanna Maurizio Cossu Feten Belhadj Boutheina Gharbi Maria Barbara Pinna Antonio Barberis Carlo Ignazio Giovanni Tuberoso Guy D’hallewin Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells Antioxidants polyphenols grapevine pomace antioxidants cyclic voltammetry prooxidant activity copper |
| title | Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells |
| title_full | Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells |
| title_fullStr | Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells |
| title_full_unstemmed | Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells |
| title_short | Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells |
| title_sort | anti and pro oxidant activity of polyphenols extracts of syrah and chardonnay grapevine pomaces on melanoma cancer cells |
| topic | polyphenols grapevine pomace antioxidants cyclic voltammetry prooxidant activity copper |
| url | https://www.mdpi.com/2076-3921/12/1/80 |
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