Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice
BackgroundObesity is associated with a decrease in testicular function, yet the effects and mechanisms relative to different stages of sexual development remain unclear. The aim of this study is to determine whether high-fat diet-induced obesity impairs male fertility during puberty and in adulthood...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.839034/full |
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| author | Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Dandan Luo Dandan Luo Dandan Luo Dandan Luo Dandan Luo Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu |
| author_facet | Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Dandan Luo Dandan Luo Dandan Luo Dandan Luo Dandan Luo Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu |
| author_sort | Xiangyu Qi |
| collection | DOAJ |
| description | BackgroundObesity is associated with a decrease in testicular function, yet the effects and mechanisms relative to different stages of sexual development remain unclear. The aim of this study is to determine whether high-fat diet-induced obesity impairs male fertility during puberty and in adulthood, and to ascertain its underlying mechanisms. This study aims to further reveal whether restoring to a normal diet can improve impaired fertility.MethodsMale mice were divided into 6 groups: the group N and H exposed to a normal diet or high-fat diet during puberty. The group NN or NH were further maintained a normal diet or exposed to high-fat diet in adulthood, the group HH or HN were further maintained high-fat diet or switched to normal diet in adulthood. Metabolic parameters, fertility parameters, testicular function parameters, TUNEL staining and testicular function-related proteins were evaluated, respectively.ResultsThe fertility of the mice in the high-fat diet group was impaired, which validated by declines in pregnancy rates and litter weight loss. Further analysis demonstrated the increased level of oxidative stress, the increased number of spermatogenic cell apoptosis and decreased number of sperm and decreased acrosome integrity. The expression of steroidogenic acute regulatory (StAR) and spermatogenesis related proteins (WT-1) decreased. Fertility among the HN group recovered, accompanied by the recovery of metabolism, fertility and testicular function parameters, StAR and WT-1 expression.ConclusionsThe findings suggest that high-fat diet-induced obesity impairs male fertility during puberty and in adulthood. The loss of acrosome integrity, the increase of oxidative stress, the increase of cells apoptosis and the down-regulation of StAR and WT-1 may be the underlying mechanisms. Switching from high-fat diets during puberty to normal diets in adulthood can improve male fertility. |
| first_indexed | 2024-04-13T16:03:28Z |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T16:03:28Z |
| publishDate | 2022-04-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-89a1c6a96ccc42a2966184f8c2b6dc192022-12-22T02:40:28ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-04-011310.3389/fendo.2022.839034839034Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male MiceXiangyu Qi0Xiangyu Qi1Xiangyu Qi2Xiangyu Qi3Xiangyu Qi4Meijie Zhang5Meijie Zhang6Meijie Zhang7Meijie Zhang8Meijie Zhang9Meijie Zhang10Mingqi Sun11Mingqi Sun12Mingqi Sun13Mingqi Sun14Mingqi Sun15Mingqi Sun16Dandan Luo17Dandan Luo18Dandan Luo19Dandan Luo20Dandan Luo21Qingbo Guan22Qingbo Guan23Qingbo Guan24Qingbo Guan25Qingbo Guan26Chunxiao Yu27Chunxiao Yu28Chunxiao Yu29Chunxiao Yu30Chunxiao Yu31Department of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaJing’an District Center Hospital, Fudan University, Shanghai, ChinaDepartment of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaGeneral Practice, Jinan Forth People’s Hospital, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, ChinaShandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaShandong Laboratory of Endocrinology and Lipid Metabolism, Shandong Provincial Hospital, Jinan, ChinaShandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital, Jinan, ChinaBackgroundObesity is associated with a decrease in testicular function, yet the effects and mechanisms relative to different stages of sexual development remain unclear. The aim of this study is to determine whether high-fat diet-induced obesity impairs male fertility during puberty and in adulthood, and to ascertain its underlying mechanisms. This study aims to further reveal whether restoring to a normal diet can improve impaired fertility.MethodsMale mice were divided into 6 groups: the group N and H exposed to a normal diet or high-fat diet during puberty. The group NN or NH were further maintained a normal diet or exposed to high-fat diet in adulthood, the group HH or HN were further maintained high-fat diet or switched to normal diet in adulthood. Metabolic parameters, fertility parameters, testicular function parameters, TUNEL staining and testicular function-related proteins were evaluated, respectively.ResultsThe fertility of the mice in the high-fat diet group was impaired, which validated by declines in pregnancy rates and litter weight loss. Further analysis demonstrated the increased level of oxidative stress, the increased number of spermatogenic cell apoptosis and decreased number of sperm and decreased acrosome integrity. The expression of steroidogenic acute regulatory (StAR) and spermatogenesis related proteins (WT-1) decreased. Fertility among the HN group recovered, accompanied by the recovery of metabolism, fertility and testicular function parameters, StAR and WT-1 expression.ConclusionsThe findings suggest that high-fat diet-induced obesity impairs male fertility during puberty and in adulthood. The loss of acrosome integrity, the increase of oxidative stress, the increase of cells apoptosis and the down-regulation of StAR and WT-1 may be the underlying mechanisms. Switching from high-fat diets during puberty to normal diets in adulthood can improve male fertility.https://www.frontiersin.org/articles/10.3389/fendo.2022.839034/fullobesityhigh fat dietrestoring to low fat dietpubertytestisfertility |
| spellingShingle | Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Xiangyu Qi Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Meijie Zhang Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Mingqi Sun Dandan Luo Dandan Luo Dandan Luo Dandan Luo Dandan Luo Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Qingbo Guan Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu Chunxiao Yu Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice Frontiers in Endocrinology obesity high fat diet restoring to low fat diet puberty testis fertility |
| title | Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice |
| title_full | Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice |
| title_fullStr | Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice |
| title_full_unstemmed | Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice |
| title_short | Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice |
| title_sort | restoring impaired fertility through diet observations of switching from high fat diet during puberty to normal diet in adulthood among obese male mice |
| topic | obesity high fat diet restoring to low fat diet puberty testis fertility |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.839034/full |
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