Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion
Abstract Pancreatic islet insulin secretion is amplified by both metabolic and receptor‐mediated signaling pathways. The incretin‐mimetic and DPPIV inhibitor anti‐diabetic drugs increase insulin secretion, but in humans this can be variable both in vitro and in vivo. We examined the correlation of G...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-04-01
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| Series: | Physiological Reports |
| Online Access: | https://doi.org/10.14814/phy2.14420 |
| _version_ | 1811225508045651968 |
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| author | Mourad Ferdaoussi Nancy Smith Haopeng Lin Austin Bautista Aliya F. Spigelman James Lyon XiaoQing Dai Jocelyn E. Manning Fox Patrick E. MacDonald |
| author_facet | Mourad Ferdaoussi Nancy Smith Haopeng Lin Austin Bautista Aliya F. Spigelman James Lyon XiaoQing Dai Jocelyn E. Manning Fox Patrick E. MacDonald |
| author_sort | Mourad Ferdaoussi |
| collection | DOAJ |
| description | Abstract Pancreatic islet insulin secretion is amplified by both metabolic and receptor‐mediated signaling pathways. The incretin‐mimetic and DPPIV inhibitor anti‐diabetic drugs increase insulin secretion, but in humans this can be variable both in vitro and in vivo. We examined the correlation of GLP‐1 induced insulin secretion from human islets with key donor characteristics, glucose‐responsiveness, and the ability of glucose to augment exocytosis in β‐cells. No clear correlation was observed between several donor or organ processing parameters and the ability of Exendin 4 to enhance insulin secretion. The ability of glucose to facilitate β‐cell exocytosis was, however, significantly correlated with responses to Exendin 4. We therefore studied the effect of impaired glucose‐dependent amplification of insulin exocytosis on responses to DPPIV inhibition (MK‐0626) in vivo using pancreas and β‐cell specific sentrin‐specific protease‐1 (SENP1) mice which exhibit impaired metabolic amplification of insulin exocytosis. Glucose tolerance was improved, and plasma insulin was increased, following either acute or 4 week treatment of wild‐type (βSENP1+/+) mice with MK‐0626. This DPPIV inhibitor was ineffective in βSENP1+/− or βSENP1−/− mice. Finally, we confirm impaired exocytotic responses of β‐cells and reduced insulin secretion from islets of βSENP1−/− mice and show that the ability of Exendin 4 to enhance exocytosis is lost in these cells. Thus, an impaired ability of glucose to amplify insulin exocytosis results in a deficient effect of DPPIV inhibition to improve in vivo insulin responses and glucose tolerance. |
| first_indexed | 2024-04-12T09:08:23Z |
| format | Article |
| id | doaj.art-89a1ec04b4254dde88da44dea078310e |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-04-12T09:08:23Z |
| publishDate | 2020-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-89a1ec04b4254dde88da44dea078310e2022-12-22T03:39:02ZengWileyPhysiological Reports2051-817X2020-04-0188n/an/a10.14814/phy2.14420Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretionMourad Ferdaoussi0Nancy Smith1Haopeng Lin2Austin Bautista3Aliya F. Spigelman4James Lyon5XiaoQing Dai6Jocelyn E. Manning Fox7Patrick E. MacDonald8Department of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaDepartment of Pharmacology and Alberta Diabetes Institute University of Alberta Edmonton AB CanadaAbstract Pancreatic islet insulin secretion is amplified by both metabolic and receptor‐mediated signaling pathways. The incretin‐mimetic and DPPIV inhibitor anti‐diabetic drugs increase insulin secretion, but in humans this can be variable both in vitro and in vivo. We examined the correlation of GLP‐1 induced insulin secretion from human islets with key donor characteristics, glucose‐responsiveness, and the ability of glucose to augment exocytosis in β‐cells. No clear correlation was observed between several donor or organ processing parameters and the ability of Exendin 4 to enhance insulin secretion. The ability of glucose to facilitate β‐cell exocytosis was, however, significantly correlated with responses to Exendin 4. We therefore studied the effect of impaired glucose‐dependent amplification of insulin exocytosis on responses to DPPIV inhibition (MK‐0626) in vivo using pancreas and β‐cell specific sentrin‐specific protease‐1 (SENP1) mice which exhibit impaired metabolic amplification of insulin exocytosis. Glucose tolerance was improved, and plasma insulin was increased, following either acute or 4 week treatment of wild‐type (βSENP1+/+) mice with MK‐0626. This DPPIV inhibitor was ineffective in βSENP1+/− or βSENP1−/− mice. Finally, we confirm impaired exocytotic responses of β‐cells and reduced insulin secretion from islets of βSENP1−/− mice and show that the ability of Exendin 4 to enhance exocytosis is lost in these cells. Thus, an impaired ability of glucose to amplify insulin exocytosis results in a deficient effect of DPPIV inhibition to improve in vivo insulin responses and glucose tolerance.https://doi.org/10.14814/phy2.14420 |
| spellingShingle | Mourad Ferdaoussi Nancy Smith Haopeng Lin Austin Bautista Aliya F. Spigelman James Lyon XiaoQing Dai Jocelyn E. Manning Fox Patrick E. MacDonald Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion Physiological Reports |
| title | Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion |
| title_full | Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion |
| title_fullStr | Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion |
| title_full_unstemmed | Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion |
| title_short | Improved glucose tolerance with DPPIV inhibition requires β‐cell SENP1 amplification of glucose‐stimulated insulin secretion |
| title_sort | improved glucose tolerance with dppiv inhibition requires β cell senp1 amplification of glucose stimulated insulin secretion |
| url | https://doi.org/10.14814/phy2.14420 |
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