Ar s enite treatment induces Hsp90 aggregates distinct from conventional stress granules in fission yeast
Various stress conditions, such as heat stress (HS) and oxidative stress, can cause biomolecular condensates represented by stress granules (SGs) via liquid-liquid phase separation. We have previously shown that Hsp90 forms aggregates in response to HS and that Hsp90 aggregates transiently co-locali...
| Main Authors: | , , |
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| 格式: | 文件 |
| 语言: | English |
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Shared Science Publishers OG
2024-07-01
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| 丛编: | Microbial Cell |
| 主题: | |
| 在线阅读: | http://microbialcell.com/researcharticles/2024a-tomimoto-microbial-cell/ |
| _version_ | 1826532047215132672 |
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| author | Naofumi Tomimoto Teruaki Takasaki Reiko Sugiura |
| author_facet | Naofumi Tomimoto Teruaki Takasaki Reiko Sugiura |
| author_sort | Naofumi Tomimoto |
| collection | DOAJ |
| description | Various stress conditions, such as heat stress (HS) and oxidative stress, can cause biomolecular condensates represented by stress granules (SGs) via liquid-liquid phase separation. We have previously shown that Hsp90 forms aggregates in response to HS and that Hsp90 aggregates transiently co-localize with SGs as visualized by Pabp. Here, we showed that arsenite, one of the well-described SG-inducing stimuli, induces Hsp90 aggregates distinct from conventional SGs in fission yeast. Arsenite induced Hsp90 granules in a dose-dependent manner, and these granules were significantly diminished by the co-treatment with a ROS scavenger N-acetyl cysteine (NAC), indicating that ROS are required for the formation of Hsp90 granules upon arsenite stress. Notably, Hsp90 granules induced by arsenite do not overlap with conventional SGs as represented by eIF4G or Pabp, while HS-induced Hsp90 granules co-localize with SGs. Nrd1, an RNA-binding protein known as a HS-induced SG component, was recruited into Hsp90 aggregates but not to the conventional SGs upon arsenite stress. The non-phosphorylatable eIF2α mutants significantly delayed the Hsp90 granule formation upon arsenite treatment. Importantly, inhibition of Hsp90 by geldanamycin impaired the Hsp90 granule formation and reduced the arsenite tolerance. Collectively, arsenite stimulates two types of distinct aggregates, namely conventional SGs and a novel type of aggregates containing Hsp90 and Nrd1, wherein Hsp90 plays a role as a center for aggregation, and stress-specific compartmentalization of biomolecular condensates. |
| first_indexed | 2025-03-14T01:45:01Z |
| format | Article |
| id | doaj.art-89a600108571494ab6266b25992ad83e |
| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2025-03-14T01:45:01Z |
| publishDate | 2024-07-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-89a600108571494ab6266b25992ad83e2025-03-12T13:46:42ZengShared Science Publishers OGMicrobial Cell2311-26382024-07-011124225310.15698/mic2024.07.829Ar s enite treatment induces Hsp90 aggregates distinct from conventional stress granules in fission yeastNaofumi Tomimoto0Teruaki Takasaki1Reiko Sugiura2Laboratory of Molecular Pharmacogenomics, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577-8502, JapanLaboratory of Molecular Pharmacogenomics, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577-8502, JapanLaboratory of Molecular Pharmacogenomics, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577-8502, JapanVarious stress conditions, such as heat stress (HS) and oxidative stress, can cause biomolecular condensates represented by stress granules (SGs) via liquid-liquid phase separation. We have previously shown that Hsp90 forms aggregates in response to HS and that Hsp90 aggregates transiently co-localize with SGs as visualized by Pabp. Here, we showed that arsenite, one of the well-described SG-inducing stimuli, induces Hsp90 aggregates distinct from conventional SGs in fission yeast. Arsenite induced Hsp90 granules in a dose-dependent manner, and these granules were significantly diminished by the co-treatment with a ROS scavenger N-acetyl cysteine (NAC), indicating that ROS are required for the formation of Hsp90 granules upon arsenite stress. Notably, Hsp90 granules induced by arsenite do not overlap with conventional SGs as represented by eIF4G or Pabp, while HS-induced Hsp90 granules co-localize with SGs. Nrd1, an RNA-binding protein known as a HS-induced SG component, was recruited into Hsp90 aggregates but not to the conventional SGs upon arsenite stress. The non-phosphorylatable eIF2α mutants significantly delayed the Hsp90 granule formation upon arsenite treatment. Importantly, inhibition of Hsp90 by geldanamycin impaired the Hsp90 granule formation and reduced the arsenite tolerance. Collectively, arsenite stimulates two types of distinct aggregates, namely conventional SGs and a novel type of aggregates containing Hsp90 and Nrd1, wherein Hsp90 plays a role as a center for aggregation, and stress-specific compartmentalization of biomolecular condensates.http://microbialcell.com/researcharticles/2024a-tomimoto-microbial-cell/hsp90aggregatesstress granulesfission yeastarsenite |
| spellingShingle | Naofumi Tomimoto Teruaki Takasaki Reiko Sugiura Ar s enite treatment induces Hsp90 aggregates distinct from conventional stress granules in fission yeast Microbial Cell hsp90 aggregates stress granules fission yeast arsenite |
| title | Ar
s
enite treatment induces
Hsp90 aggregates
distinct
from
conventional
stress granules
in fission yeast |
| title_full | Ar
s
enite treatment induces
Hsp90 aggregates
distinct
from
conventional
stress granules
in fission yeast |
| title_fullStr | Ar
s
enite treatment induces
Hsp90 aggregates
distinct
from
conventional
stress granules
in fission yeast |
| title_full_unstemmed | Ar
s
enite treatment induces
Hsp90 aggregates
distinct
from
conventional
stress granules
in fission yeast |
| title_short | Ar
s
enite treatment induces
Hsp90 aggregates
distinct
from
conventional
stress granules
in fission yeast |
| title_sort | ar s enite treatment induces hsp90 aggregates distinct from conventional stress granules in fission yeast |
| topic | hsp90 aggregates stress granules fission yeast arsenite |
| url | http://microbialcell.com/researcharticles/2024a-tomimoto-microbial-cell/ |
| work_keys_str_mv | AT naofumitomimoto arsenitetreatmentinduceshsp90aggregatesdistinctfromconventionalstressgranulesinfissionyeast AT teruakitakasaki arsenitetreatmentinduceshsp90aggregatesdistinctfromconventionalstressgranulesinfissionyeast AT reikosugiura arsenitetreatmentinduceshsp90aggregatesdistinctfromconventionalstressgranulesinfissionyeast |