Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan
Stripe (yellow) rust, caused by <i>Puccinia striiformis</i> f.sp. <i>tritici</i> (<i>Pst</i>), is a fungal disease that presents one of the most serious threats to the wheat crops, causing severe yield losses worldwide, including Kazakhstan. The objectives of this...
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MDPI AG
2021-10-01
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| author | Alma Kokhmetova Aralbek Rsaliyev Angelina Malysheva Makpal Atishova Madina Kumarbayeva Zhenis Keishilov |
| author_facet | Alma Kokhmetova Aralbek Rsaliyev Angelina Malysheva Makpal Atishova Madina Kumarbayeva Zhenis Keishilov |
| author_sort | Alma Kokhmetova |
| collection | DOAJ |
| description | Stripe (yellow) rust, caused by <i>Puccinia striiformis</i> f.sp. <i>tritici</i> (<i>Pst</i>), is a fungal disease that presents one of the most serious threats to the wheat crops, causing severe yield losses worldwide, including Kazakhstan. The objectives of this study were to: (1) evaluate a winter wheat collection for stripe rust resistance during an adult plant growth stage, (2) identify the presence of selected <i>Yr</i> genes using linked molecular markers in wheat germplasm, (3) identify potentially useful resistant wheat genotypes among leading cultivars and advanced breeding lines. This study evaluated 70 winter wheat genotypes for stripe rust resistance. According to the field reactions, 42 entries (60%) had R or MR reactions including 27 breeding lines (38.6%) and 15 (21.4%) cultivars. Twenty-eight breeding lines/cultivars (40.0%) were susceptible in both years. According to the average coefficient of infection value (ACI) six genotypes were regarded as possessing high level of adult plant resistance. Cultivars/lines carrying <i>Yr10</i> alone or in combination with other <i>Yr</i> resistance genes provided resistance to stripe rust. Eleven breeding lines showed <5% disease severity in both years. Linked marker analysis revealed the presence of several gene and gene complexes (<i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr17/Lr37/Sr38</i> and <i>Yr18/Lr34</i>). Among a collection of 70 winter wheat breeding lines and cultivars produced in Kazakhstan three stripe rust resistance genes (<i>Yr10</i>, <i>Yr5</i> and <i>Yr15</i>) demonstrated high frequency occurrence (31.4%, 14.0% and 7.0%, respectively). The most abundant was gene <i>Yr10</i> identified in 22 genotypes. It was followed by the <i>Yr5</i> gene, which conferred resistance in 14 lines (20%) and <i>Yr18</i> gene-11 lines (15.7%). <i>Yr15</i> was identified in 7 genotypes. <i>Yr17/Lr37/Sr38</i> gene complex was found in 2 entries. Among 70 evaluated germplasm sources, 42 disease resistant entries are potentially useful resistant wheat genotypes. These carriers of different <i>Yr</i> genes can be used directly in breeding programs to improve stripe rust resistance of winter wheat. Marker-assisted selection can be efficiently applied to develop wheat cultivars with effective gene combinations that would directly assist in developing durable resistance in Kazakhstan. |
| first_indexed | 2024-03-10T05:08:21Z |
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| spelling | doaj.art-89a714ccdf0e4b858e6a312d91d923df2023-11-23T01:03:18ZengMDPI AGPlants2223-77472021-10-011011230310.3390/plants10112303Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from KazakhstanAlma Kokhmetova0Aralbek Rsaliyev1Angelina Malysheva2Makpal Atishova3Madina Kumarbayeva4Zhenis Keishilov5Institute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanResearch Institute of Biological Safety Problems, Gvardeiskiy 080409, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanStripe (yellow) rust, caused by <i>Puccinia striiformis</i> f.sp. <i>tritici</i> (<i>Pst</i>), is a fungal disease that presents one of the most serious threats to the wheat crops, causing severe yield losses worldwide, including Kazakhstan. The objectives of this study were to: (1) evaluate a winter wheat collection for stripe rust resistance during an adult plant growth stage, (2) identify the presence of selected <i>Yr</i> genes using linked molecular markers in wheat germplasm, (3) identify potentially useful resistant wheat genotypes among leading cultivars and advanced breeding lines. This study evaluated 70 winter wheat genotypes for stripe rust resistance. According to the field reactions, 42 entries (60%) had R or MR reactions including 27 breeding lines (38.6%) and 15 (21.4%) cultivars. Twenty-eight breeding lines/cultivars (40.0%) were susceptible in both years. According to the average coefficient of infection value (ACI) six genotypes were regarded as possessing high level of adult plant resistance. Cultivars/lines carrying <i>Yr10</i> alone or in combination with other <i>Yr</i> resistance genes provided resistance to stripe rust. Eleven breeding lines showed <5% disease severity in both years. Linked marker analysis revealed the presence of several gene and gene complexes (<i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr17/Lr37/Sr38</i> and <i>Yr18/Lr34</i>). Among a collection of 70 winter wheat breeding lines and cultivars produced in Kazakhstan three stripe rust resistance genes (<i>Yr10</i>, <i>Yr5</i> and <i>Yr15</i>) demonstrated high frequency occurrence (31.4%, 14.0% and 7.0%, respectively). The most abundant was gene <i>Yr10</i> identified in 22 genotypes. It was followed by the <i>Yr5</i> gene, which conferred resistance in 14 lines (20%) and <i>Yr18</i> gene-11 lines (15.7%). <i>Yr15</i> was identified in 7 genotypes. <i>Yr17/Lr37/Sr38</i> gene complex was found in 2 entries. Among 70 evaluated germplasm sources, 42 disease resistant entries are potentially useful resistant wheat genotypes. These carriers of different <i>Yr</i> genes can be used directly in breeding programs to improve stripe rust resistance of winter wheat. Marker-assisted selection can be efficiently applied to develop wheat cultivars with effective gene combinations that would directly assist in developing durable resistance in Kazakhstan.https://www.mdpi.com/2223-7747/10/11/2303wheat<i>Triticum aestivum</i> L.stripe rust<i>Puccinia striiformis</i>resistance genesmolecular markers |
| spellingShingle | Alma Kokhmetova Aralbek Rsaliyev Angelina Malysheva Makpal Atishova Madina Kumarbayeva Zhenis Keishilov Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan Plants wheat <i>Triticum aestivum</i> L. stripe rust <i>Puccinia striiformis</i> resistance genes molecular markers |
| title | Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan |
| title_full | Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan |
| title_fullStr | Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan |
| title_full_unstemmed | Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan |
| title_short | Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars and Breeding Lines from Kazakhstan |
| title_sort | identification of stripe rust resistance genes in common wheat cultivars and breeding lines from kazakhstan |
| topic | wheat <i>Triticum aestivum</i> L. stripe rust <i>Puccinia striiformis</i> resistance genes molecular markers |
| url | https://www.mdpi.com/2223-7747/10/11/2303 |
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