A Single-Cell Atlas of an Early Mongolian Sheep Embryo
Cell types have been established during organogenesis based on early mouse embryos. However, our understanding of cell types and molecular mechanisms in the early embryo development of Mongolian sheep has been hampered. This study presents the first comprehensive single-cell transcriptomic character...
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| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/9/543 |
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| author | Tingyi He Wenrui Guo Guang Yang Hong Su Aolei Dou Lu Chen Teng Ma Jie Su Moning Liu Budeng Su Wangmei Qi Haijun Li Wei Mao Xiumei Wang Xihe Li Yanyan Yang Yongli Song Guifang Cao |
| author_facet | Tingyi He Wenrui Guo Guang Yang Hong Su Aolei Dou Lu Chen Teng Ma Jie Su Moning Liu Budeng Su Wangmei Qi Haijun Li Wei Mao Xiumei Wang Xihe Li Yanyan Yang Yongli Song Guifang Cao |
| author_sort | Tingyi He |
| collection | DOAJ |
| description | Cell types have been established during organogenesis based on early mouse embryos. However, our understanding of cell types and molecular mechanisms in the early embryo development of Mongolian sheep has been hampered. This study presents the first comprehensive single-cell transcriptomic characterization at E16 in Ujumqin sheep and Hulunbuir short-tailed sheep. Thirteen major cell types were identified at E16 in Ujumqin sheep, and eight major cell types were identified at E16 in Hulunbuir short-tailed sheep. Function enrichment analysis showed that several pathways were significantly enriched in the TGF-beta signaling pathway, the Hippo signaling pathway, the platelet activation pathway, the riboflavin metabolism pathway, the Wnt signaling pathway, regulation of the actin cytoskeleton, and the insulin signaling pathway in the notochord cluster. Glutathione metabolism, glyoxylate, and dicarboxylate metabolism, the citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, the cell cycle, the Hippo signaling pathway, the Hedgehog signaling pathway, the FoxO signaling pathway, the JAK-STAT signaling pathway, and the Wnt signaling pathway were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster, and paraxial mesoderm cluster were found to be highly associated with tail development. Pseudo-time analysis demonstrated that the mesenchyme can translate to the notochord in Ujumqin sheep. Molecular assays revealed that the Hippo signaling pathway was enriched in Ujumqin sheep. This comprehensive single-cell map revealed previously unrecognized signaling pathways that will further our understanding of the mechanism of short-tailed sheep formation. |
| first_indexed | 2024-03-10T21:52:40Z |
| format | Article |
| id | doaj.art-89a7c2eaa4af4de58ffce805ecb7456c |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T21:52:40Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-89a7c2eaa4af4de58ffce805ecb7456c2023-11-19T13:20:38ZengMDPI AGVeterinary Sciences2306-73812023-08-0110954310.3390/vetsci10090543A Single-Cell Atlas of an Early Mongolian Sheep EmbryoTingyi He0Wenrui Guo1Guang Yang2Hong Su3Aolei Dou4Lu Chen5Teng Ma6Jie Su7Moning Liu8Budeng Su9Wangmei Qi10Haijun Li11Wei Mao12Xiumei Wang13Xihe Li14Yanyan Yang15Yongli Song16Guifang Cao17Inner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010020, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Medical Neurobiology, Inner Mongolia Medical University, Huhhot 010030, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010020, ChinaInstitute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010031, ChinaThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010020, ChinaInner Mongolia Key Laboratory of Basic Veterinary Medicine, Key Laboratory of Animal Embryo, and Development Engineering Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCell types have been established during organogenesis based on early mouse embryos. However, our understanding of cell types and molecular mechanisms in the early embryo development of Mongolian sheep has been hampered. This study presents the first comprehensive single-cell transcriptomic characterization at E16 in Ujumqin sheep and Hulunbuir short-tailed sheep. Thirteen major cell types were identified at E16 in Ujumqin sheep, and eight major cell types were identified at E16 in Hulunbuir short-tailed sheep. Function enrichment analysis showed that several pathways were significantly enriched in the TGF-beta signaling pathway, the Hippo signaling pathway, the platelet activation pathway, the riboflavin metabolism pathway, the Wnt signaling pathway, regulation of the actin cytoskeleton, and the insulin signaling pathway in the notochord cluster. Glutathione metabolism, glyoxylate, and dicarboxylate metabolism, the citrate cycle, thyroid hormone synthesis, pyruvate metabolism, cysteine and methionine metabolism, thermogenesis, and the VEGF signaling pathway were significantly enriched in the spinal cord cluster. Steroid biosynthesis, riboflavin metabolism, the cell cycle, the Hippo signaling pathway, the Hedgehog signaling pathway, the FoxO signaling pathway, the JAK-STAT signaling pathway, and the Wnt signaling pathway were significantly enriched in the paraxial mesoderm cluster. The notochord cluster, spinal cord cluster, and paraxial mesoderm cluster were found to be highly associated with tail development. Pseudo-time analysis demonstrated that the mesenchyme can translate to the notochord in Ujumqin sheep. Molecular assays revealed that the Hippo signaling pathway was enriched in Ujumqin sheep. This comprehensive single-cell map revealed previously unrecognized signaling pathways that will further our understanding of the mechanism of short-tailed sheep formation.https://www.mdpi.com/2306-7381/10/9/543Mongolian sheepembryoscRNA-seqHippo signaling pathway |
| spellingShingle | Tingyi He Wenrui Guo Guang Yang Hong Su Aolei Dou Lu Chen Teng Ma Jie Su Moning Liu Budeng Su Wangmei Qi Haijun Li Wei Mao Xiumei Wang Xihe Li Yanyan Yang Yongli Song Guifang Cao A Single-Cell Atlas of an Early Mongolian Sheep Embryo Veterinary Sciences Mongolian sheep embryo scRNA-seq Hippo signaling pathway |
| title | A Single-Cell Atlas of an Early Mongolian Sheep Embryo |
| title_full | A Single-Cell Atlas of an Early Mongolian Sheep Embryo |
| title_fullStr | A Single-Cell Atlas of an Early Mongolian Sheep Embryo |
| title_full_unstemmed | A Single-Cell Atlas of an Early Mongolian Sheep Embryo |
| title_short | A Single-Cell Atlas of an Early Mongolian Sheep Embryo |
| title_sort | single cell atlas of an early mongolian sheep embryo |
| topic | Mongolian sheep embryo scRNA-seq Hippo signaling pathway |
| url | https://www.mdpi.com/2306-7381/10/9/543 |
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