Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria
Abstract Antibiotic-resistant bacterial strains and the consequent surge in infections caused by them have become major public health concerns. Silver nanoparticles (AgNPs) exhibit antibacterial properties and have wide applications in biomedical sciences. In this study, AgNPs were synthesized in th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-023-01619-7 |
| _version_ | 1797556243056820224 |
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| author | Muhammad Adil Siyab Alam Urooj Amin Irfan Ullah Mian Muhammad Muti Ullah Asma Rehman Tariq Khan |
| author_facet | Muhammad Adil Siyab Alam Urooj Amin Irfan Ullah Mian Muhammad Muti Ullah Asma Rehman Tariq Khan |
| author_sort | Muhammad Adil |
| collection | DOAJ |
| description | Abstract Antibiotic-resistant bacterial strains and the consequent surge in infections caused by them have become major public health concerns. Silver nanoparticles (AgNPs) exhibit antibacterial properties and have wide applications in biomedical sciences. In this study, AgNPs were synthesized in the presence of antibiotics: Ceftazidime (Cft), Cefotaxime (Cef), Ceftriaxone (Cfx), and Cefepime (Cpm), along with the extract of Mentha longifolia. Mentha longifolia-based AgNPs were kept as the control for all experiments. The associated metabolites, structural properties, surface charges, and antibacterial activity of the AgNPs were also evaluated. Overall, a blue-shift of SPR peaks was observed for control AgNPs (λmax = 421 nm, 422 nm, 426 nm, and 406 nm for Cft-AgNPs, Cef-AgNPs, Cfx-AgNPs, and Cpm-AgNPs, respectively), compared to the control (λmax = 438 nm). Fourier-transform infrared spectroscopy showed that antibiotic-based AgNPs had distinct peaks that corresponded to the respective antibiotics, which were not observed in the control. XRD analysis showed that there were observed changes in crystallinity in antibiotic-based AgNPs compared to the control. TEM images revealed that all samples had spherical nanoparticles with different sizes and distributions compared to the control. The Zeta potential for extract-based AgNPs was − 33.6 mV, compared to -19.6 mV for Cft-AgNPs, -2 mV for Cef-AgNPs, -21.1 mV for Cfx-AgNPs, and − 24.2 mV for Cpm-AgNPs. The increase in the PDI value for antibiotic-based AgNPs also showed a highly polydisperse distribution. However, the antibiotic-AgNPs conjugates showed significantly higher activity against pathogenic bacteria. The addition of antibiotics to AgNPs brought significant changes in structural properties and antibacterial activities. |
| first_indexed | 2024-03-10T16:59:05Z |
| format | Article |
| id | doaj.art-89acbd3f08404a4d9689ad3442259362 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-03-10T16:59:05Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-89acbd3f08404a4d9689ad34422593622023-11-20T11:01:31ZengSpringerOpenAMB Express2191-08552023-10-0113111410.1186/s13568-023-01619-7Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteriaMuhammad Adil0Siyab Alam1Urooj Amin2Irfan Ullah3Mian Muhammad4Muti Ullah5Asma Rehman6Tariq Khan7Department of Biotechnology, University of MalakandDepartment of Biotechnology, University of MalakandDepartment of Biotechnology, University of MalakandDepartment of Biotechnology, University of MalakandDepartment of Chemistry, University of MalakandInstitute of Pathology and Diagnostic Medicine, Khyber Medical UniversityNanobiotechnology Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE)Department of Biotechnology, University of MalakandAbstract Antibiotic-resistant bacterial strains and the consequent surge in infections caused by them have become major public health concerns. Silver nanoparticles (AgNPs) exhibit antibacterial properties and have wide applications in biomedical sciences. In this study, AgNPs were synthesized in the presence of antibiotics: Ceftazidime (Cft), Cefotaxime (Cef), Ceftriaxone (Cfx), and Cefepime (Cpm), along with the extract of Mentha longifolia. Mentha longifolia-based AgNPs were kept as the control for all experiments. The associated metabolites, structural properties, surface charges, and antibacterial activity of the AgNPs were also evaluated. Overall, a blue-shift of SPR peaks was observed for control AgNPs (λmax = 421 nm, 422 nm, 426 nm, and 406 nm for Cft-AgNPs, Cef-AgNPs, Cfx-AgNPs, and Cpm-AgNPs, respectively), compared to the control (λmax = 438 nm). Fourier-transform infrared spectroscopy showed that antibiotic-based AgNPs had distinct peaks that corresponded to the respective antibiotics, which were not observed in the control. XRD analysis showed that there were observed changes in crystallinity in antibiotic-based AgNPs compared to the control. TEM images revealed that all samples had spherical nanoparticles with different sizes and distributions compared to the control. The Zeta potential for extract-based AgNPs was − 33.6 mV, compared to -19.6 mV for Cft-AgNPs, -2 mV for Cef-AgNPs, -21.1 mV for Cfx-AgNPs, and − 24.2 mV for Cpm-AgNPs. The increase in the PDI value for antibiotic-based AgNPs also showed a highly polydisperse distribution. However, the antibiotic-AgNPs conjugates showed significantly higher activity against pathogenic bacteria. The addition of antibiotics to AgNPs brought significant changes in structural properties and antibacterial activities.https://doi.org/10.1186/s13568-023-01619-7Silver nanoparticlesCephalosporinsAntibioticsMentha longifoliaAntibiotic resistant bacteria |
| spellingShingle | Muhammad Adil Siyab Alam Urooj Amin Irfan Ullah Mian Muhammad Muti Ullah Asma Rehman Tariq Khan Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria AMB Express Silver nanoparticles Cephalosporins Antibiotics Mentha longifolia Antibiotic resistant bacteria |
| title | Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria |
| title_full | Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria |
| title_fullStr | Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria |
| title_full_unstemmed | Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria |
| title_short | Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria |
| title_sort | efficient green silver nanoparticles antibiotic combinations against antibiotic resistant bacteria |
| topic | Silver nanoparticles Cephalosporins Antibiotics Mentha longifolia Antibiotic resistant bacteria |
| url | https://doi.org/10.1186/s13568-023-01619-7 |
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