Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease
Background and objective: Polycystic liver disease (PCLD) represents a group of genetic disorders that include autosomal dominant polycystic kidney disease (ADPKD) and isolated polycystic liver disease (iPCLD). There is currently no definitive treatment except for liver transplantation. The aim of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Aran Ediciones
2016-02-01
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| Series: | Revista Espanola de Enfermedades Digestivas |
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| Online Access: | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082016000200004&lng=en&tlng=en |
| _version_ | 1828768520333164544 |
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| author | Li Dingyang Shi Xiaoju Zhao Lijing Liang Zuowen Xie Shuli Wang Guangyi |
| author_facet | Li Dingyang Shi Xiaoju Zhao Lijing Liang Zuowen Xie Shuli Wang Guangyi |
| author_sort | Li Dingyang |
| collection | DOAJ |
| description | Background and objective: Polycystic liver disease (PCLD) represents a group of genetic disorders that include autosomal dominant polycystic kidney disease (ADPKD) and isolated polycystic liver disease (iPCLD). There is currently no definitive treatment except for liver transplantation. The aim of this study was to assess the expression level of aquaporin 1 (AQP1) on the PCLD cysts with different sizes and provide the potential therapeutic target. Methods: We collected 3 normal bile ducts, and recruited 8 patients with simple liver cyst disease, 24 patients with ADPKD, and 17 patients with iPCLD. AQP1 expression in different types of cyst walls and in normal bile ducts was detected using real time quantitative PCR, western blot and immunofluorescence staining. We also compared AQP1 expression levels in cysts of different sizes. Besides, ionic concentrations, pH and osmolality of cyst fluid were analyzed. Results: The results showed that AQP1 expression in PCLD cysts was significantly higher than that in simple liver cysts and the normal bile ducts. In addition, a comparable increasing trend was found in cysts of smaller sizes to cysts of larger sizes. pH values, the sodium and chloride concentrations were higher in cyst fluid than that in the serum. Conclusions: AQP1 was overexpressed in cystic cholangiocytes. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. These observations offered a direction into the molecular mechanisms of cyst expansion and maybe provide new treatment strategies to reduce fluid secretion into liver cysts. |
| first_indexed | 2024-12-11T13:32:11Z |
| format | Article |
| id | doaj.art-89b244c0f9e24c8a871f5a20f7dc0496 |
| institution | Directory Open Access Journal |
| issn | 1130-0108 |
| language | English |
| last_indexed | 2024-12-11T13:32:11Z |
| publishDate | 2016-02-01 |
| publisher | Aran Ediciones |
| record_format | Article |
| series | Revista Espanola de Enfermedades Digestivas |
| spelling | doaj.art-89b244c0f9e24c8a871f5a20f7dc04962022-12-22T01:05:14ZengAran EdicionesRevista Espanola de Enfermedades Digestivas1130-01082016-02-0110827178S1130-01082016000200004Overexpression of Aquaporin 1 on cysts of patients with polycystic liver diseaseLi Dingyang0Shi Xiaoju1Zhao Lijing2Liang Zuowen3Xie Shuli4Wang Guangyi5Jilin UniversityJilin UniversityJilin UniversityJilin UniversityJilin UniversityJilin UniversityBackground and objective: Polycystic liver disease (PCLD) represents a group of genetic disorders that include autosomal dominant polycystic kidney disease (ADPKD) and isolated polycystic liver disease (iPCLD). There is currently no definitive treatment except for liver transplantation. The aim of this study was to assess the expression level of aquaporin 1 (AQP1) on the PCLD cysts with different sizes and provide the potential therapeutic target. Methods: We collected 3 normal bile ducts, and recruited 8 patients with simple liver cyst disease, 24 patients with ADPKD, and 17 patients with iPCLD. AQP1 expression in different types of cyst walls and in normal bile ducts was detected using real time quantitative PCR, western blot and immunofluorescence staining. We also compared AQP1 expression levels in cysts of different sizes. Besides, ionic concentrations, pH and osmolality of cyst fluid were analyzed. Results: The results showed that AQP1 expression in PCLD cysts was significantly higher than that in simple liver cysts and the normal bile ducts. In addition, a comparable increasing trend was found in cysts of smaller sizes to cysts of larger sizes. pH values, the sodium and chloride concentrations were higher in cyst fluid than that in the serum. Conclusions: AQP1 was overexpressed in cystic cholangiocytes. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. These observations offered a direction into the molecular mechanisms of cyst expansion and maybe provide new treatment strategies to reduce fluid secretion into liver cysts.http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082016000200004&lng=en&tlng=enAquaporin 1Simple cystic liver diseaseAutosomal dominant polycystic kidney diseaseIsolated polycystic liver diseasePolycystic liver disease |
| spellingShingle | Li Dingyang Shi Xiaoju Zhao Lijing Liang Zuowen Xie Shuli Wang Guangyi Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease Revista Espanola de Enfermedades Digestivas Aquaporin 1 Simple cystic liver disease Autosomal dominant polycystic kidney disease Isolated polycystic liver disease Polycystic liver disease |
| title | Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease |
| title_full | Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease |
| title_fullStr | Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease |
| title_full_unstemmed | Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease |
| title_short | Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease |
| title_sort | overexpression of aquaporin 1 on cysts of patients with polycystic liver disease |
| topic | Aquaporin 1 Simple cystic liver disease Autosomal dominant polycystic kidney disease Isolated polycystic liver disease Polycystic liver disease |
| url | http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1130-01082016000200004&lng=en&tlng=en |
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