Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand
Abstract Background: Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devic...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-022-01904-0 |
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| author | Kanaporn Poltep Juthamas Phadungsombat Nathamon Kosoltanapiwat Borimas Hanboonkunupakarn Witthawat Wiriyarat Sarin Suwanpakdee Phirom Prompiram Emi E. Nakayama Keita Suzuki Hisahiko Iwamoto Tatsuo Shioda Pornsawan Leaungwutiwong |
| author_facet | Kanaporn Poltep Juthamas Phadungsombat Nathamon Kosoltanapiwat Borimas Hanboonkunupakarn Witthawat Wiriyarat Sarin Suwanpakdee Phirom Prompiram Emi E. Nakayama Keita Suzuki Hisahiko Iwamoto Tatsuo Shioda Pornsawan Leaungwutiwong |
| author_sort | Kanaporn Poltep |
| collection | DOAJ |
| description | Abstract Background: Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1st and TKK 2nd kits, based on dengue virus (DENV) nonstructural protein 1 detection. However, these TKK kits were evaluated mainly using DENV type 2 clinical specimens collected in Bangladesh, and further validation using clinical specimens of other serotypes was needed. Methods: In the present study, one of the TKK kits, TKK 2nd, was evaluated using 10 DENV-1, 10 DENV-2, 4 DENV-3, 16 DENV-4, and 10 zika virus-infected clinical specimens collected in Bangkok, Thailand. Results: The TKK 2nd kit successfully detected all four DENV serotypes in patient serum specimens and did not show any cross-reactivities against zika virus serum specimens. The IgM and/or IgG anti-DENV antibodies were detected in seven serum specimens, but did not seem to affect the results of antigen detection in the TKK 2nd kit. Conclusion: The results showed that the TKK 2nd kit successfully detected all four DENV serotypes in clinical specimens and confirmed the potential of the kit for dengue diagnosis in endemic countries. |
| first_indexed | 2024-03-11T11:07:37Z |
| format | Article |
| id | doaj.art-89b55fb4761740f4a1f9ee7bba1a3f41 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-03-11T11:07:37Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-89b55fb4761740f4a1f9ee7bba1a3f412023-11-12T12:06:44ZengBMCVirology Journal1743-422X2022-10-011911610.1186/s12985-022-01904-0Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, ThailandKanaporn Poltep0Juthamas Phadungsombat1Nathamon Kosoltanapiwat2Borimas Hanboonkunupakarn3Witthawat Wiriyarat4Sarin Suwanpakdee5Phirom Prompiram6Emi E. Nakayama7Keita Suzuki8Hisahiko Iwamoto9Tatsuo Shioda10Pornsawan Leaungwutiwong11Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol UniversityMahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol UniversityDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol UniversityThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE), Faculty of Veterinary Science, Mahidol UniversityThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE), Faculty of Veterinary Science, Mahidol UniversityThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE), Faculty of Veterinary Science, Mahidol UniversityMahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol UniversityPOCT Business Unit, TANAKA Kikinzoku Kogyo K.KPOCT Business Unit, TANAKA Kikinzoku Kogyo K.KMahidol-Osaka Center for Infectious Diseases (MOCID), Faculty of Tropical Medicine, Mahidol UniversityDepartment of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol UniversityAbstract Background: Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1st and TKK 2nd kits, based on dengue virus (DENV) nonstructural protein 1 detection. However, these TKK kits were evaluated mainly using DENV type 2 clinical specimens collected in Bangladesh, and further validation using clinical specimens of other serotypes was needed. Methods: In the present study, one of the TKK kits, TKK 2nd, was evaluated using 10 DENV-1, 10 DENV-2, 4 DENV-3, 16 DENV-4, and 10 zika virus-infected clinical specimens collected in Bangkok, Thailand. Results: The TKK 2nd kit successfully detected all four DENV serotypes in patient serum specimens and did not show any cross-reactivities against zika virus serum specimens. The IgM and/or IgG anti-DENV antibodies were detected in seven serum specimens, but did not seem to affect the results of antigen detection in the TKK 2nd kit. Conclusion: The results showed that the TKK 2nd kit successfully detected all four DENV serotypes in clinical specimens and confirmed the potential of the kit for dengue diagnosis in endemic countries.https://doi.org/10.1186/s12985-022-01904-0DengueDiagnosisNS1ICTRDT |
| spellingShingle | Kanaporn Poltep Juthamas Phadungsombat Nathamon Kosoltanapiwat Borimas Hanboonkunupakarn Witthawat Wiriyarat Sarin Suwanpakdee Phirom Prompiram Emi E. Nakayama Keita Suzuki Hisahiko Iwamoto Tatsuo Shioda Pornsawan Leaungwutiwong Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand Virology Journal Dengue Diagnosis NS1 ICT RDT |
| title | Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand |
| title_full | Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand |
| title_fullStr | Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand |
| title_full_unstemmed | Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand |
| title_short | Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand |
| title_sort | performance of the nonstructural 1 antigen rapid test for detecting all four denv serotypes in clinical specimens from bangkok thailand |
| topic | Dengue Diagnosis NS1 ICT RDT |
| url | https://doi.org/10.1186/s12985-022-01904-0 |
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