Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells
In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression vectors, it is still influenced by the low st...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.879222/full |
| _version_ | 1818193665122631680 |
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| author | Mian Wei Mian Wei Chun-Liu Mi Chang-Qin Jing Tian-Yun Wang |
| author_facet | Mian Wei Mian Wei Chun-Liu Mi Chang-Qin Jing Tian-Yun Wang |
| author_sort | Mian Wei |
| collection | DOAJ |
| description | In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression vectors, it is still influenced by the low stability and efficiency of gene integration. Transposons are mobile genetic elements that can be inserted and cleaved within the genome and can change their inserting position. The transposon vector system can be applied to establish a stable pool of cells with high efficiency in RTPs production through facilitating the integration of gene of interest into transcriptionally active sites under screening pressure. Here, the structure and optimization of transposon vector system and its application in expressing RTPs at high level in mammalian cells are reviewed. |
| first_indexed | 2024-12-12T00:50:00Z |
| format | Article |
| id | doaj.art-89b5af4fbe0e4fdc94fed7a6c4df0c3f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T00:50:00Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-89b5af4fbe0e4fdc94fed7a6c4df0c3f2022-12-22T00:44:01ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-05-011010.3389/fbioe.2022.879222879222Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian CellsMian Wei0Mian Wei1Chun-Liu Mi2Chang-Qin Jing3Tian-Yun Wang4School of Life Science and Technology, Xinxiang Medical University, Xinxiang, ChinaInternational Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang, ChinaInternational Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang, ChinaSchool of Life Science and Technology, Xinxiang Medical University, Xinxiang, ChinaInternational Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang, ChinaIn recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression vectors, it is still influenced by the low stability and efficiency of gene integration. Transposons are mobile genetic elements that can be inserted and cleaved within the genome and can change their inserting position. The transposon vector system can be applied to establish a stable pool of cells with high efficiency in RTPs production through facilitating the integration of gene of interest into transcriptionally active sites under screening pressure. Here, the structure and optimization of transposon vector system and its application in expressing RTPs at high level in mammalian cells are reviewed.https://www.frontiersin.org/articles/10.3389/fbioe.2022.879222/fullrecombinant therapeutic proteinmammalian cellstransposon vectortransposasePiggyBac |
| spellingShingle | Mian Wei Mian Wei Chun-Liu Mi Chang-Qin Jing Tian-Yun Wang Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells Frontiers in Bioengineering and Biotechnology recombinant therapeutic protein mammalian cells transposon vector transposase PiggyBac |
| title | Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells |
| title_full | Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells |
| title_fullStr | Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells |
| title_full_unstemmed | Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells |
| title_short | Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells |
| title_sort | progress of transposon vector system for production of recombinant therapeutic proteins in mammalian cells |
| topic | recombinant therapeutic protein mammalian cells transposon vector transposase PiggyBac |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.879222/full |
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