Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study
The appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X18300283 |
| _version_ | 1797205222536249344 |
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| author | Ahyar Ahmad Rosana Agus Muh. Nasrum Massi Rosdiana Natzir Radha Madhyastha Harish Kumar Madhyastha Masugi Maruyama |
| author_facet | Ahyar Ahmad Rosana Agus Muh. Nasrum Massi Rosdiana Natzir Radha Madhyastha Harish Kumar Madhyastha Masugi Maruyama |
| author_sort | Ahyar Ahmad |
| collection | DOAJ |
| description | The appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop the epidemic of TB. Some M. tuberculosis antigens, one of which is MPT83, have been examined as TB vaccine candidate. MPT83 antigen, which is very immunogenic in lipoprotein micro bacteria, is identified as surface cell interrelated to antigen with cytometry circulation. Having TB resistance from BCG vaccine, MPT83 is considered TB vaccine candidate that can protect people against TB at adult age. The purpose of this research is to conduct amplification of MPT83 antigen cloning, and expression of its antigen on E. coli bacteria. From the result of the research, it is expected that raw material to produce TB vaccine as well as a high-quality antigen can be obtained. The band of DNA in PCR product is 660 bp, while the one in pGEMT-Easy-Mpt83 recombinant plasmid is 3678 bp. This is expressed in E. coli BL21 strain and produces 48 kDa protein as well as GST-MPT83 fusion protein. Keywords: Tuberculosis, Cloning, GST-MPT83 |
| first_indexed | 2024-04-24T08:47:42Z |
| format | Article |
| id | doaj.art-89b8c5caf7a740f184d315c62d5ddf01 |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T08:47:42Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-89b8c5caf7a740f184d315c62d5ddf012024-04-16T13:12:43ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2018-12-01162335340Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary studyAhyar Ahmad0Rosana Agus1Muh. Nasrum Massi2Rosdiana Natzir3Radha Madhyastha4Harish Kumar Madhyastha5Masugi Maruyama6Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Perintis Kemerdekaan Street km. 10 Tamalanrea, Makassar 90245, Indonesia; Laboratory of Research Centre and Developing of Sciences, Faculty of Mathematic and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia; Corresponding author at: Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Perintis Kemerdekaan Street km. 10 Tamalanrea, Makassar 90245, Indonesia.Biology Department, Mathematic and Natural Science Faculty, Hasanuddin Univesity, Perintis Kemerdekaan Street km. 10 Tamalanrea, Makassar 90245, IndonesiaMicrobiology Department, Faculty of Medicine, Hasanuddin Univesity, Perintis Kemerdekaan Street km. 10 Tamalanrea, Makassar 90245, IndonesiaBiochemistry Department, Faculty of Medicine, Hasanuddin Univesity, Perintis Kemerdekaan Street km. 10 Tamalanrea, Makassar 90245, IndonesiaDept. of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, JapanDept. of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, JapanDept. of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, JapanThe appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop the epidemic of TB. Some M. tuberculosis antigens, one of which is MPT83, have been examined as TB vaccine candidate. MPT83 antigen, which is very immunogenic in lipoprotein micro bacteria, is identified as surface cell interrelated to antigen with cytometry circulation. Having TB resistance from BCG vaccine, MPT83 is considered TB vaccine candidate that can protect people against TB at adult age. The purpose of this research is to conduct amplification of MPT83 antigen cloning, and expression of its antigen on E. coli bacteria. From the result of the research, it is expected that raw material to produce TB vaccine as well as a high-quality antigen can be obtained. The band of DNA in PCR product is 660 bp, while the one in pGEMT-Easy-Mpt83 recombinant plasmid is 3678 bp. This is expressed in E. coli BL21 strain and produces 48 kDa protein as well as GST-MPT83 fusion protein. Keywords: Tuberculosis, Cloning, GST-MPT83http://www.sciencedirect.com/science/article/pii/S1687157X18300283 |
| spellingShingle | Ahyar Ahmad Rosana Agus Muh. Nasrum Massi Rosdiana Natzir Radha Madhyastha Harish Kumar Madhyastha Masugi Maruyama Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study Journal of Genetic Engineering and Biotechnology |
| title | Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study |
| title_full | Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study |
| title_fullStr | Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study |
| title_full_unstemmed | Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study |
| title_short | Cloning and expression of MPT83 gene from Mycobacterium tuberculosis in E. coli BL21 as vaccine candidate of tuberculosis: A preliminary study |
| title_sort | cloning and expression of mpt83 gene from mycobacterium tuberculosis in e coli bl21 as vaccine candidate of tuberculosis a preliminary study |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X18300283 |
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