Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua)
The rice leaf folder, Marasmia exigua (Butler), is dangerous pests and negatively affecting the rice production worldwide. Protein elicitors are considered to be bio-factors that induce rice resistance against herbivores. The present study was performed with two protein elicitors, Hrip 1 (extracted...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721003141 |
| _version_ | 1818932518513016832 |
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| author | Abdul Basit Muhammad Farhan Muhammad Essa Mureed Abbas Yong Wang De-Gang Zhao Amin U. Maridha Muhammad Amjad Bashir Arif Hussain Abdul Hanan Reem A Alajmi Saeed Al-Ashram Billy M. Hargis |
| author_facet | Abdul Basit Muhammad Farhan Muhammad Essa Mureed Abbas Yong Wang De-Gang Zhao Amin U. Maridha Muhammad Amjad Bashir Arif Hussain Abdul Hanan Reem A Alajmi Saeed Al-Ashram Billy M. Hargis |
| author_sort | Abdul Basit |
| collection | DOAJ |
| description | The rice leaf folder, Marasmia exigua (Butler), is dangerous pests and negatively affecting the rice production worldwide. Protein elicitors are considered to be bio-factors that induce rice resistance against herbivores. The present study was performed with two protein elicitors, Hrip 1 (extracted from Alternaria tenuissima) and PebB1 (from Beauveria bassiana) to study their efficacy against the rice leaf folder. Three different concentrations of Hrip 1 (68.88, 46.69 and 28.44 µg/ml) and PebB1 (56.66, 39.76 and 32.62 µg/ml) were assigned to evaluate the developmental time, survival rate, and fecundity of M. exigua. Both elicitors were applied to the three-leaf stage of the rice plant where newly emerged adult insects were released. Bioassay results showed that after the application of protein elicitors, the life cycle and survival rate of the rice leaf folder were prolonged, while fecundity decreased. The concentration of protein had a significant (p < 0.05) influence on ontogeny. In addition to ontogeny, the expression profile of jasmonic acid, salicylic acid, and ethylene associated with signalling pathway, which indicated that exogenous application of both elicitors produced a significant up-regulation in all the genes associated in signalling pathways of the plant. The plants treated with elicitors produced resistance against M. exigua (as compared to the control). This in vitro study invites new research into Hrip 1 and PebB1 as having prophylactic potential against rice leaf folder, and suggest that both protein elicitors can be used as a novel biological control tool for M. exigua. |
| first_indexed | 2024-12-20T04:33:46Z |
| format | Article |
| id | doaj.art-89bab0de805a48c58630825bf5cb52da |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-20T04:33:46Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-89bab0de805a48c58630825bf5cb52da2022-12-21T19:53:19ZengElsevierJournal of King Saud University: Science1018-36472021-12-01338101652Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua)Abdul Basit0Muhammad Farhan1Muhammad Essa2Mureed Abbas3Yong Wang4De-Gang Zhao5Amin U. Maridha6Muhammad Amjad Bashir7Arif Hussain8Abdul Hanan9Reem A Alajmi10Saeed Al-Ashram11Billy M. Hargis12Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, ChinaDepartment of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, ChinaMedical Officer BHU Talpur Tehsil Kot Chutta District Dera Ghazi Khan Punjab, PakistanInstitute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; Corresponding author at: Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, China.Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, China; Key Laboratory of Plant Resources Conservation and Germ-plasm Innovation in Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Plant Resources Conservation and Germ-plasm Innovation in Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaDepartment of Botany, University of Chittagong, Chittagong, BangladeshDepartment of Plant Protection Faculty of Agricultural Sciences, Ghazi University Dera Ghazi Khan Punjab, PakistanDepartment of Soil and Environmental Science, Ghazi University, Dera Ghazi Khan, PakistanDepartment of Entomology, University of Agriculture, Faisalabad, PakistanDepartment of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia; Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt; College of Life Science and Engineering , FoshanUniversity, Foshan China; Faculty of Science KafrelsheikhUniversity Kafer El Sheikh EgyptDepartment of Poultry Science, University of Arkansas, Fayetteville, AR, USAThe rice leaf folder, Marasmia exigua (Butler), is dangerous pests and negatively affecting the rice production worldwide. Protein elicitors are considered to be bio-factors that induce rice resistance against herbivores. The present study was performed with two protein elicitors, Hrip 1 (extracted from Alternaria tenuissima) and PebB1 (from Beauveria bassiana) to study their efficacy against the rice leaf folder. Three different concentrations of Hrip 1 (68.88, 46.69 and 28.44 µg/ml) and PebB1 (56.66, 39.76 and 32.62 µg/ml) were assigned to evaluate the developmental time, survival rate, and fecundity of M. exigua. Both elicitors were applied to the three-leaf stage of the rice plant where newly emerged adult insects were released. Bioassay results showed that after the application of protein elicitors, the life cycle and survival rate of the rice leaf folder were prolonged, while fecundity decreased. The concentration of protein had a significant (p < 0.05) influence on ontogeny. In addition to ontogeny, the expression profile of jasmonic acid, salicylic acid, and ethylene associated with signalling pathway, which indicated that exogenous application of both elicitors produced a significant up-regulation in all the genes associated in signalling pathways of the plant. The plants treated with elicitors produced resistance against M. exigua (as compared to the control). This in vitro study invites new research into Hrip 1 and PebB1 as having prophylactic potential against rice leaf folder, and suggest that both protein elicitors can be used as a novel biological control tool for M. exigua.http://www.sciencedirect.com/science/article/pii/S1018364721003141Plants resistanceProtein elicitorsRice leaf folderSignalling pathway |
| spellingShingle | Abdul Basit Muhammad Farhan Muhammad Essa Mureed Abbas Yong Wang De-Gang Zhao Amin U. Maridha Muhammad Amjad Bashir Arif Hussain Abdul Hanan Reem A Alajmi Saeed Al-Ashram Billy M. Hargis Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) Journal of King Saud University: Science Plants resistance Protein elicitors Rice leaf folder Signalling pathway |
| title | Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) |
| title_full | Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) |
| title_fullStr | Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) |
| title_full_unstemmed | Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) |
| title_short | Effect of two protein elicitors extracted from Alternaria tenuissima and Beauveria bassiana against rice leaf folder (Marasmia exigua) |
| title_sort | effect of two protein elicitors extracted from alternaria tenuissima and beauveria bassiana against rice leaf folder marasmia exigua |
| topic | Plants resistance Protein elicitors Rice leaf folder Signalling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721003141 |
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