Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection
Priming agents are plant defence-inducing compounds which can prompt a state of protection but may also aid in plant growth and interactions with beneficial microbes. The synthetic strigolactones (±)-GR24 and Nijmegen-1 were evaluated as potential priming agents for induced resistance against <i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3202 |
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| author | Dominic Vogel Paul Hills John P. Moore |
| author_facet | Dominic Vogel Paul Hills John P. Moore |
| author_sort | Dominic Vogel |
| collection | DOAJ |
| description | Priming agents are plant defence-inducing compounds which can prompt a state of protection but may also aid in plant growth and interactions with beneficial microbes. The synthetic strigolactones (±)-GR24 and Nijmegen-1 were evaluated as potential priming agents for induced resistance against <i>Botrytis cinerea</i> in tobacco and grapevine plants. The growth and stress response profiles of <i>B. cinerea</i> to strigolactones were also investigated. Soil drench treatment with strigolactones induced resistance in greenhouse-grown tobacco plants and restricted lesion development. The mode of action appeared to function by priming redox-associated compounds to produce an anti-oxidant protective response for limiting the infection. The results obtained in the in vitro assays mirrored that of the greenhouse-grown plants. Exposure of <i>B. cinerea</i> to the strigolactones resulted in increased hyphal branching, with (±)-GR24 stimulating a stronger effect than Nijmegen-1 by affecting colony diameter and radial growth. An oxidative stress response was observed, with <i>B. cinerea</i> exhibiting increased ROS and SOD levels when grown with strigolactones. This study identified the application of strigolactones as potential priming agents to induce disease resistance in both tobacco and grapevine plants. In addition, strigolactones may alter the ROS homeostasis of <i>B. cinerea</i>, resulting in both morphological and physiological changes, thereby reducing virulence. |
| first_indexed | 2024-03-10T22:13:28Z |
| format | Article |
| id | doaj.art-89bf134f779d441da643f1d2281a45c1 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:13:28Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-89bf134f779d441da643f1d2281a45c12023-11-19T12:31:31ZengMDPI AGPlants2223-77472023-09-011218320210.3390/plants12183202Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> InfectionDominic Vogel0Paul Hills1John P. Moore2South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Stellenbosch 7600, South AfricaInstitute for Plant Biotechnology, Department of Genetics, Faculty of AgriSciences, Stellenbosch University, Stellenbosch 7602, South AfricaSouth African Grape and Wine Research Institute, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Stellenbosch 7600, South AfricaPriming agents are plant defence-inducing compounds which can prompt a state of protection but may also aid in plant growth and interactions with beneficial microbes. The synthetic strigolactones (±)-GR24 and Nijmegen-1 were evaluated as potential priming agents for induced resistance against <i>Botrytis cinerea</i> in tobacco and grapevine plants. The growth and stress response profiles of <i>B. cinerea</i> to strigolactones were also investigated. Soil drench treatment with strigolactones induced resistance in greenhouse-grown tobacco plants and restricted lesion development. The mode of action appeared to function by priming redox-associated compounds to produce an anti-oxidant protective response for limiting the infection. The results obtained in the in vitro assays mirrored that of the greenhouse-grown plants. Exposure of <i>B. cinerea</i> to the strigolactones resulted in increased hyphal branching, with (±)-GR24 stimulating a stronger effect than Nijmegen-1 by affecting colony diameter and radial growth. An oxidative stress response was observed, with <i>B. cinerea</i> exhibiting increased ROS and SOD levels when grown with strigolactones. This study identified the application of strigolactones as potential priming agents to induce disease resistance in both tobacco and grapevine plants. In addition, strigolactones may alter the ROS homeostasis of <i>B. cinerea</i>, resulting in both morphological and physiological changes, thereby reducing virulence.https://www.mdpi.com/2223-7747/12/18/3202<i>Botrytis cinerea</i>plant fitnessreactive oxygen speciesstrigolactoneshyphal branching |
| spellingShingle | Dominic Vogel Paul Hills John P. Moore Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection Plants <i>Botrytis cinerea</i> plant fitness reactive oxygen species strigolactones hyphal branching |
| title | Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection |
| title_full | Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection |
| title_fullStr | Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection |
| title_full_unstemmed | Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection |
| title_short | Strigolactones GR-24 and Nijmegen Applications Result in Reduced Susceptibility of Tobacco and Grapevine Plantlets to <i>Botrytis cinerea</i> Infection |
| title_sort | strigolactones gr 24 and nijmegen applications result in reduced susceptibility of tobacco and grapevine plantlets to i botrytis cinerea i infection |
| topic | <i>Botrytis cinerea</i> plant fitness reactive oxygen species strigolactones hyphal branching |
| url | https://www.mdpi.com/2223-7747/12/18/3202 |
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