Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression.
AIMS: It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3634741?pdf=render |
| _version_ | 1828851612229042176 |
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| author | Xiu-Jing Yue Lei-Bo Xu Man-Sheng Zhu Rui Zhang Chao Liu |
| author_facet | Xiu-Jing Yue Lei-Bo Xu Man-Sheng Zhu Rui Zhang Chao Liu |
| author_sort | Xiu-Jing Yue |
| collection | DOAJ |
| description | AIMS: It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However, whether NGF-β promotes tumor progression in human cholangiocarcinoma requires further investigation. Therefore, we aimed to determine the effects of NGF-β on the progression of human cholangiocarcinoma. METHODS: Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo-NGF-β-shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. RESULTS: Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. CONCLUSIONS: We concluded that NGF-β promoted tumor progression in human cholangiocarcinoma QBC939 cells. Our results provided a new concept to understand the role of NGF-β in cholangiocarcinoma progression, and might provide important information for the development of new targeted therapies in human cholangiocarcinoma. |
| first_indexed | 2024-12-12T23:35:42Z |
| format | Article |
| id | doaj.art-89c465295ed8404fb06bc11fee0d9b7d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:35:42Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-89c465295ed8404fb06bc11fee0d9b7d2022-12-22T00:07:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6202410.1371/journal.pone.0062024Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression.Xiu-Jing YueLei-Bo XuMan-Sheng ZhuRui ZhangChao LiuAIMS: It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However, whether NGF-β promotes tumor progression in human cholangiocarcinoma requires further investigation. Therefore, we aimed to determine the effects of NGF-β on the progression of human cholangiocarcinoma. METHODS: Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo-NGF-β-shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. RESULTS: Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. CONCLUSIONS: We concluded that NGF-β promoted tumor progression in human cholangiocarcinoma QBC939 cells. Our results provided a new concept to understand the role of NGF-β in cholangiocarcinoma progression, and might provide important information for the development of new targeted therapies in human cholangiocarcinoma.http://europepmc.org/articles/PMC3634741?pdf=render |
| spellingShingle | Xiu-Jing Yue Lei-Bo Xu Man-Sheng Zhu Rui Zhang Chao Liu Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. PLoS ONE |
| title | Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. |
| title_full | Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. |
| title_fullStr | Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. |
| title_full_unstemmed | Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. |
| title_short | Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. |
| title_sort | over expression of nerve growth factor β in human cholangiocarcinoma qbc939 cells promote tumor progression |
| url | http://europepmc.org/articles/PMC3634741?pdf=render |
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