Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.
BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intrace...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2680040?pdf=render |
| _version_ | 1818765702324027392 |
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| author | Sebastian D Schuck Henrik Mueller Frank Kunitz Albert Neher Harald Hoffmann Kees L C M Franken Dirk Repsilber Tom H M Ottenhoff Stefan H E Kaufmann Marc Jacobsen |
| author_facet | Sebastian D Schuck Henrik Mueller Frank Kunitz Albert Neher Harald Hoffmann Kees L C M Franken Dirk Repsilber Tom H M Ottenhoff Stefan H E Kaufmann Marc Jacobsen |
| author_sort | Sebastian D Schuck |
| collection | DOAJ |
| description | BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI. |
| first_indexed | 2024-12-18T08:22:17Z |
| format | Article |
| id | doaj.art-89c64a6f3f85446eb6ba1e2aa4faffe2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T08:22:17Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-89c64a6f3f85446eb6ba1e2aa4faffe22022-12-21T21:14:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0145e559010.1371/journal.pone.0005590Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.Sebastian D SchuckHenrik MuellerFrank KunitzAlbert NeherHarald HoffmannKees L C M FrankenDirk RepsilberTom H M OttenhoffStefan H E KaufmannMarc JacobsenBACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.http://europepmc.org/articles/PMC2680040?pdf=render |
| spellingShingle | Sebastian D Schuck Henrik Mueller Frank Kunitz Albert Neher Harald Hoffmann Kees L C M Franken Dirk Repsilber Tom H M Ottenhoff Stefan H E Kaufmann Marc Jacobsen Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. PLoS ONE |
| title | Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| title_full | Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| title_fullStr | Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| title_full_unstemmed | Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| title_short | Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. |
| title_sort | identification of t cell antigens specific for latent mycobacterium tuberculosis infection |
| url | http://europepmc.org/articles/PMC2680040?pdf=render |
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