Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms
A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2018-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300607&lng=en&tlng=en |
| _version_ | 1830205822987141120 |
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| author | Lella Kalyani Chava Venkata Nageswara Rao |
| author_facet | Lella Kalyani Chava Venkata Nageswara Rao |
| author_sort | Lella Kalyani |
| collection | DOAJ |
| description | A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the different stress conditions were separated from both drugs. This validated method was applied for the simultaneous estimation of cefepime and amikacin in commercially available formulation sample. |
| first_indexed | 2024-12-18T04:00:40Z |
| format | Article |
| id | doaj.art-89c6ccda3eb34beab21ed27cdc8b6b13 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-18T04:00:40Z |
| publishDate | 2018-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-89c6ccda3eb34beab21ed27cdc8b6b132022-12-21T21:21:41ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902018-11-0154310.1590/s2175-97902018000317258S1984-82502018000300607Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage formsLella KalyaniChava Venkata Nageswara RaoA simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the different stress conditions were separated from both drugs. This validated method was applied for the simultaneous estimation of cefepime and amikacin in commercially available formulation sample.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300607&lng=en&tlng=enCefepime/method development and validationAmikacin/method development and validationRP-HPLC |
| spellingShingle | Lella Kalyani Chava Venkata Nageswara Rao Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms Brazilian Journal of Pharmaceutical Sciences Cefepime/method development and validation Amikacin/method development and validation RP-HPLC |
| title | Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| title_full | Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| title_fullStr | Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| title_full_unstemmed | Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| title_short | Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| title_sort | stability indicating rp hplc method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms |
| topic | Cefepime/method development and validation Amikacin/method development and validation RP-HPLC |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300607&lng=en&tlng=en |
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