Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina.
Ribosome-inactivating proteins (RIPs) are endowed with several medicinal properties, including antiviral activity. We demonstrate here that the recently identified type I RIP from Momordica balsamina also possesses antiviral activity, as determined by viral growth curve assays and single-round infec...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3764001?pdf=render |
| _version_ | 1818508114367873024 |
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| author | Inderdeep Kaur Munish Puri Zahra Ahmed Fabien P Blanchet Bastien Mangeat Vincent Piguet |
| author_facet | Inderdeep Kaur Munish Puri Zahra Ahmed Fabien P Blanchet Bastien Mangeat Vincent Piguet |
| author_sort | Inderdeep Kaur |
| collection | DOAJ |
| description | Ribosome-inactivating proteins (RIPs) are endowed with several medicinal properties, including antiviral activity. We demonstrate here that the recently identified type I RIP from Momordica balsamina also possesses antiviral activity, as determined by viral growth curve assays and single-round infection experiments. Importantly, this activity is at play even as doses where the RIP has no cytotoxic effect. In addition, balsamin inhibits HIV-1 replication not only in T cell lines but also in human primary CD4(+) T cells. This antiviral compound exerts its activity at a viral replicative step occurring later than reverse-transcription, most likely on viral protein translation, prior to viral budding and release. Finally, we demonstrate that balsamin antiviral activity is broad since it also impedes influenza virus replication. Altogether our results demonstrate that type I RIP can exert a potent anti-HIV-1 activity which paves the way for new therapeutic avenues for the treatment of viral infections. |
| first_indexed | 2024-12-10T22:27:19Z |
| format | Article |
| id | doaj.art-89c9da2cc0f34cfe9ed4baff0c38c0df |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:27:19Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-89c9da2cc0f34cfe9ed4baff0c38c0df2022-12-22T01:31:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7378010.1371/journal.pone.0073780Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina.Inderdeep KaurMunish PuriZahra AhmedFabien P BlanchetBastien MangeatVincent PiguetRibosome-inactivating proteins (RIPs) are endowed with several medicinal properties, including antiviral activity. We demonstrate here that the recently identified type I RIP from Momordica balsamina also possesses antiviral activity, as determined by viral growth curve assays and single-round infection experiments. Importantly, this activity is at play even as doses where the RIP has no cytotoxic effect. In addition, balsamin inhibits HIV-1 replication not only in T cell lines but also in human primary CD4(+) T cells. This antiviral compound exerts its activity at a viral replicative step occurring later than reverse-transcription, most likely on viral protein translation, prior to viral budding and release. Finally, we demonstrate that balsamin antiviral activity is broad since it also impedes influenza virus replication. Altogether our results demonstrate that type I RIP can exert a potent anti-HIV-1 activity which paves the way for new therapeutic avenues for the treatment of viral infections.http://europepmc.org/articles/PMC3764001?pdf=render |
| spellingShingle | Inderdeep Kaur Munish Puri Zahra Ahmed Fabien P Blanchet Bastien Mangeat Vincent Piguet Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. PLoS ONE |
| title | Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. |
| title_full | Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. |
| title_fullStr | Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. |
| title_full_unstemmed | Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. |
| title_short | Inhibition of HIV-1 replication by balsamin, a ribosome inactivating protein of Momordica balsamina. |
| title_sort | inhibition of hiv 1 replication by balsamin a ribosome inactivating protein of momordica balsamina |
| url | http://europepmc.org/articles/PMC3764001?pdf=render |
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