Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions
The intracellular homeostasis of lyophilized lactic acid bacteria (LAB) is destroyed by extreme cold stress, resulting in decreased stability. This study aimed to verify the validity of collagen as a potential protective agent for improving microbial stability deteriorated by freezing. The collagen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/3/177 |
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| author | Sun-Il Kim Jin-Woo Kim Ki-Tae Kim Chang-Ho Kang |
| author_facet | Sun-Il Kim Jin-Woo Kim Ki-Tae Kim Chang-Ho Kang |
| author_sort | Sun-Il Kim |
| collection | DOAJ |
| description | The intracellular homeostasis of lyophilized lactic acid bacteria (LAB) is destroyed by extreme cold stress, resulting in decreased stability. This study aimed to verify the validity of collagen as a potential protective agent for improving microbial stability deteriorated by freezing. The collagen types used in this study were low molecular weight collagen (LC) of less than 1000 Da and low molecular weight collagen-peptide (LCP) of less than 300 Da. By the accelerated stability test according to the addition of each collagen type, a 3% LCP displaying a protective effect on the viability of various LAB strains (<i>Lactoplantibacillus plantarum</i> MG989, <i>Lactococcus lactis</i> MG5125, <i>Enterococcus faecium</i> MG5232, <i>Bifidobacterium animalis</i> ssp. <i>lactis</i> MG741, and <i>Streptococcus thermophilus</i> MG5140) was finally selected. It was evaluated whether LCP enhances bacterial stability, survivability in the gastrointestinal (GI) tract, and heat resistance. LCP significantly improved the viability of all strains in the GI tract compared to sucrose and skim milk, which are conventional protective agents. Based on morphological observations, LCP was uniformly coated on the cell surface, resulting in protective effects against multiple external stress stimuli. Such findings indicate the applicability of LCP as an unprecedented protective agent, which can improve the stability of various probiotics with antifreeze effects. |
| first_indexed | 2024-03-10T07:41:42Z |
| format | Article |
| id | doaj.art-89cfee9ebaf74843b432f7c12ee6f13d |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T07:41:42Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-89cfee9ebaf74843b432f7c12ee6f13d2023-11-22T13:00:17ZengMDPI AGFermentation2311-56372021-09-017317710.3390/fermentation7030177Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal ConditionsSun-Il Kim0Jin-Woo Kim1Ki-Tae Kim2Chang-Ho Kang3Mediogen, Co., Ltd., Bio Valley 1-ro, Jecheon-si 27159, KoreaMediogen, Co., Ltd., Bio Valley 1-ro, Jecheon-si 27159, KoreaMediogen, Co., Ltd., Bio Valley 1-ro, Jecheon-si 27159, KoreaMediogen, Co., Ltd., Bio Valley 1-ro, Jecheon-si 27159, KoreaThe intracellular homeostasis of lyophilized lactic acid bacteria (LAB) is destroyed by extreme cold stress, resulting in decreased stability. This study aimed to verify the validity of collagen as a potential protective agent for improving microbial stability deteriorated by freezing. The collagen types used in this study were low molecular weight collagen (LC) of less than 1000 Da and low molecular weight collagen-peptide (LCP) of less than 300 Da. By the accelerated stability test according to the addition of each collagen type, a 3% LCP displaying a protective effect on the viability of various LAB strains (<i>Lactoplantibacillus plantarum</i> MG989, <i>Lactococcus lactis</i> MG5125, <i>Enterococcus faecium</i> MG5232, <i>Bifidobacterium animalis</i> ssp. <i>lactis</i> MG741, and <i>Streptococcus thermophilus</i> MG5140) was finally selected. It was evaluated whether LCP enhances bacterial stability, survivability in the gastrointestinal (GI) tract, and heat resistance. LCP significantly improved the viability of all strains in the GI tract compared to sucrose and skim milk, which are conventional protective agents. Based on morphological observations, LCP was uniformly coated on the cell surface, resulting in protective effects against multiple external stress stimuli. Such findings indicate the applicability of LCP as an unprecedented protective agent, which can improve the stability of various probiotics with antifreeze effects.https://www.mdpi.com/2311-5637/7/3/177antifreezecollagen-peptidefreeze-dryinggastrointestinal tractlactic acid bacteriaprobiotics |
| spellingShingle | Sun-Il Kim Jin-Woo Kim Ki-Tae Kim Chang-Ho Kang Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions Fermentation antifreeze collagen-peptide freeze-drying gastrointestinal tract lactic acid bacteria probiotics |
| title | Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions |
| title_full | Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions |
| title_fullStr | Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions |
| title_full_unstemmed | Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions |
| title_short | Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions |
| title_sort | survivability of collagen peptide microencapsulated lactic acid bacteria during storage and simulated gastrointestinal conditions |
| topic | antifreeze collagen-peptide freeze-drying gastrointestinal tract lactic acid bacteria probiotics |
| url | https://www.mdpi.com/2311-5637/7/3/177 |
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