Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts
<i>Bryonia alba</i> L. is the only <i>Bryonia</i> species found in Romanian flora, being known as a remedy for inflammatory pathologies or for its hepatoprotective and adaptogen activities. The present investigation studied the flavonoid composition and antioxidant activities...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-04-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/8/4/108 |
| _version_ | 1797712409409880064 |
|---|---|
| author | Irina Ielciu Michel Frédérich Daniela Hanganu Luc Angenot Neli-Kinga Olah Allison Ledoux Gianina Crișan Ramona Păltinean |
| author_facet | Irina Ielciu Michel Frédérich Daniela Hanganu Luc Angenot Neli-Kinga Olah Allison Ledoux Gianina Crișan Ramona Păltinean |
| author_sort | Irina Ielciu |
| collection | DOAJ |
| description | <i>Bryonia alba</i> L. is the only <i>Bryonia</i> species found in Romanian flora, being known as a remedy for inflammatory pathologies or for its hepatoprotective and adaptogen activities. The present investigation studied the flavonoid composition and antioxidant activities of the aerial parts of this species. Flavonoid profile was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant capacity was assessed by various methods, testing different antioxidant mechanisms: DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC (silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human cancerous and healthy cell lines. Anti-plasmodial tests were performed on two strains of <i>Plasmodium falciparum</i>. Whole organism toxicity was assessed on zebrafish larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin, isoorientin, and isovitexin as the major flavonoids in the composition of tested samples. Significant results were obtained for all antioxidant capacity assays. The cytotoxicity tests proved the absence of cellular and parasitic toxicity and these results were confirmed by the lack of toxicity on the zebrafish larvae model. This study proves a promising potential of the aerial parts of <i>Bryonia alba</i> L. as antioxidant agents. |
| first_indexed | 2024-03-12T07:21:23Z |
| format | Article |
| id | doaj.art-89d1c0d0634e44b79d1250de08809023 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:21:23Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-89d1c0d0634e44b79d1250de088090232023-09-02T22:23:28ZengMDPI AGAntioxidants2076-39212019-04-018410810.3390/antiox8040108antiox8040108Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial PartsIrina Ielciu0Michel Frédérich1Daniela Hanganu2Luc Angenot3Neli-Kinga Olah4Allison Ledoux5Gianina Crișan6Ramona Păltinean7Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hat, ieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaLaboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 4000 Liège, BelgiumDepartment of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 400010 Cluj-Napoca, RomaniaLaboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 4000 Liège, BelgiumPlantExtrakt Ltd., Rădaia, 407059 Cluj-Napoca, RomaniaLaboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 4000 Liège, BelgiumDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hat, ieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hat, ieganu” University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania<i>Bryonia alba</i> L. is the only <i>Bryonia</i> species found in Romanian flora, being known as a remedy for inflammatory pathologies or for its hepatoprotective and adaptogen activities. The present investigation studied the flavonoid composition and antioxidant activities of the aerial parts of this species. Flavonoid profile was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant capacity was assessed by various methods, testing different antioxidant mechanisms: DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC (silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human cancerous and healthy cell lines. Anti-plasmodial tests were performed on two strains of <i>Plasmodium falciparum</i>. Whole organism toxicity was assessed on zebrafish larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin, isoorientin, and isovitexin as the major flavonoids in the composition of tested samples. Significant results were obtained for all antioxidant capacity assays. The cytotoxicity tests proved the absence of cellular and parasitic toxicity and these results were confirmed by the lack of toxicity on the zebrafish larvae model. This study proves a promising potential of the aerial parts of <i>Bryonia alba</i> L. as antioxidant agents.https://www.mdpi.com/2076-3921/8/4/108flavonoidsHPLC-DADantioxidanttoxicity |
| spellingShingle | Irina Ielciu Michel Frédérich Daniela Hanganu Luc Angenot Neli-Kinga Olah Allison Ledoux Gianina Crișan Ramona Păltinean Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts Antioxidants flavonoids HPLC-DAD antioxidant toxicity |
| title | Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts |
| title_full | Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts |
| title_fullStr | Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts |
| title_full_unstemmed | Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts |
| title_short | Flavonoid Analysis and Antioxidant Activities of the <em>Bryonia alba</em> L. Aerial Parts |
| title_sort | flavonoid analysis and antioxidant activities of the em bryonia alba em l aerial parts |
| topic | flavonoids HPLC-DAD antioxidant toxicity |
| url | https://www.mdpi.com/2076-3921/8/4/108 |
| work_keys_str_mv | AT irinaielciu flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT michelfrederich flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT danielahanganu flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT lucangenot flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT nelikingaolah flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT allisonledoux flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT gianinacrisan flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts AT ramonapaltinean flavonoidanalysisandantioxidantactivitiesoftheembryoniaalbaemlaerialparts |