Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass
Skeletal muscle is a highly plastic organ that is necessary for homeostasis and health of the human body. The size of skeletal muscle changes in response to intrinsic and extrinsic stimuli. Although protein-coding RNAs including myostatin, NF-κβ, and insulin-like growth factor-1 (I...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/5/1628 |
| _version_ | 1811212498730221568 |
|---|---|
| author | Keisuke Hitachi Masashi Nakatani Shiori Funasaki Ikumi Hijikata Mizuki Maekawa Masahiko Honda Kunihiro Tsuchida |
| author_facet | Keisuke Hitachi Masashi Nakatani Shiori Funasaki Ikumi Hijikata Mizuki Maekawa Masahiko Honda Kunihiro Tsuchida |
| author_sort | Keisuke Hitachi |
| collection | DOAJ |
| description | Skeletal muscle is a highly plastic organ that is necessary for homeostasis and health of the human body. The size of skeletal muscle changes in response to intrinsic and extrinsic stimuli. Although protein-coding RNAs including myostatin, NF-κβ, and insulin-like growth factor-1 (IGF-1), have pivotal roles in determining the skeletal muscle mass, the role of long non-coding RNAs (lncRNAs) in the regulation of skeletal muscle mass remains to be elucidated. Here, we performed expression profiling of nine skeletal muscle differentiation-related lncRNAs (<i>DRR</i>, <i>DUM1</i>, <i>linc-MD1</i>, <i>linc-YY1</i>, <i>LncMyod</i>, <i>Neat1</i>, <i>Myoparr</i>, <i>Malat1</i>, and <i>SRA</i>) and three genomic imprinting-related lncRNAs (<i>Gtl2</i>, <i>H19</i>, and <i>IG-DMR</i>) in mouse skeletal muscle. The expression levels of these lncRNAs were examined by quantitative RT-PCR in six skeletal muscle atrophy models (denervation, casting, tail suspension, dexamethasone-administration, cancer cachexia, and fasting) and two skeletal muscle hypertrophy models (mechanical overload and deficiency of the <i>myostatin</i> gene). Cluster analyses of these lncRNA expression levels were successfully used to categorize the muscle atrophy models into two sub-groups. In addition, the expression of <i>Gtl2</i>, <i>IG-DMR</i>, and <i>DUM1</i> was altered along with changes in the skeletal muscle size. The overview of the expression levels of lncRNAs in multiple muscle atrophy and hypertrophy models provides a novel insight into the role of lncRNAs in determining the skeletal muscle mass. |
| first_indexed | 2024-04-12T05:29:02Z |
| format | Article |
| id | doaj.art-89d2327734934fb6be5e7d554202590b |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T05:29:02Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-89d2327734934fb6be5e7d554202590b2022-12-22T03:46:08ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01215162810.3390/ijms21051628ijms21051628Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle MassKeisuke Hitachi0Masashi Nakatani1Shiori Funasaki2Ikumi Hijikata3Mizuki Maekawa4Masahiko Honda5Kunihiro Tsuchida6Division for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanDepartment of Biochemistry, Kindai University Faculty of Medicine, Osaka-Sayama 589-8511, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science (ICMS), Fujita Health University, Toyoake 470-1192, JapanSkeletal muscle is a highly plastic organ that is necessary for homeostasis and health of the human body. The size of skeletal muscle changes in response to intrinsic and extrinsic stimuli. Although protein-coding RNAs including myostatin, NF-κβ, and insulin-like growth factor-1 (IGF-1), have pivotal roles in determining the skeletal muscle mass, the role of long non-coding RNAs (lncRNAs) in the regulation of skeletal muscle mass remains to be elucidated. Here, we performed expression profiling of nine skeletal muscle differentiation-related lncRNAs (<i>DRR</i>, <i>DUM1</i>, <i>linc-MD1</i>, <i>linc-YY1</i>, <i>LncMyod</i>, <i>Neat1</i>, <i>Myoparr</i>, <i>Malat1</i>, and <i>SRA</i>) and three genomic imprinting-related lncRNAs (<i>Gtl2</i>, <i>H19</i>, and <i>IG-DMR</i>) in mouse skeletal muscle. The expression levels of these lncRNAs were examined by quantitative RT-PCR in six skeletal muscle atrophy models (denervation, casting, tail suspension, dexamethasone-administration, cancer cachexia, and fasting) and two skeletal muscle hypertrophy models (mechanical overload and deficiency of the <i>myostatin</i> gene). Cluster analyses of these lncRNA expression levels were successfully used to categorize the muscle atrophy models into two sub-groups. In addition, the expression of <i>Gtl2</i>, <i>IG-DMR</i>, and <i>DUM1</i> was altered along with changes in the skeletal muscle size. The overview of the expression levels of lncRNAs in multiple muscle atrophy and hypertrophy models provides a novel insight into the role of lncRNAs in determining the skeletal muscle mass.https://www.mdpi.com/1422-0067/21/5/1628long non-coding rnasdisuse atrophymuscle wastingmuscle hypertrophymyostatinskeletal muscle mass |
| spellingShingle | Keisuke Hitachi Masashi Nakatani Shiori Funasaki Ikumi Hijikata Mizuki Maekawa Masahiko Honda Kunihiro Tsuchida Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass International Journal of Molecular Sciences long non-coding rnas disuse atrophy muscle wasting muscle hypertrophy myostatin skeletal muscle mass |
| title | Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass |
| title_full | Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass |
| title_fullStr | Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass |
| title_full_unstemmed | Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass |
| title_short | Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass |
| title_sort | expression levels of long non coding rnas change in models of altered muscle activity and muscle mass |
| topic | long non-coding rnas disuse atrophy muscle wasting muscle hypertrophy myostatin skeletal muscle mass |
| url | https://www.mdpi.com/1422-0067/21/5/1628 |
| work_keys_str_mv | AT keisukehitachi expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT masashinakatani expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT shiorifunasaki expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT ikumihijikata expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT mizukimaekawa expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT masahikohonda expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass AT kunihirotsuchida expressionlevelsoflongnoncodingrnaschangeinmodelsofalteredmuscleactivityandmusclemass |