Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation
Tangor, an important citrus type, is a hybrid of orange and mandarin and possesses their advantageous characteristics. Fruit quality is an important factor limiting the development of the citrus industry and highly depends on fruit development and ripening programs. However, fruit development and qu...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5457 |
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| author | Xiaoyi Bi Ling Liao Lijun Deng Zhenghua Jin Zehao Huang Guochao Sun Bo Xiong Zhihui Wang |
| author_facet | Xiaoyi Bi Ling Liao Lijun Deng Zhenghua Jin Zehao Huang Guochao Sun Bo Xiong Zhihui Wang |
| author_sort | Xiaoyi Bi |
| collection | DOAJ |
| description | Tangor, an important citrus type, is a hybrid of orange and mandarin and possesses their advantageous characteristics. Fruit quality is an important factor limiting the development of the citrus industry and highly depends on fruit development and ripening programs. However, fruit development and quality formation have not been completely explored in mandarin-orange hybrids. We sequenced the metabolome and transcriptome of three mandarin-orange hybrid cultivars at the early fruiting [90 days after full bloom (DAFB)], color change (180 DAFB), and ripening (270 DAFB) stages. Metabolome sequencing was performed to preliminarily identify the accumulation patterns of primary and secondary metabolites related to fruit quality and hormones regulating fruit development. Transcriptome analysis showed that many genes related to primary metabolism, secondary metabolism, cell wall metabolism, phytohormones, and transcriptional regulation were up-regulated in all three cultivars during fruit development and ripening. Additionally, multiple key genes were identified that may play a role in sucrose, citric acid and flavonoid accumulation, cell wall modification, and abscisic acid signaling, which may provide a valuable resource for future research on enhancement of fruit quality of hybrid citrus. Overall, this study provides new insights into the molecular basis of pulp growth and development regulation and fruit quality formation in mandarin-orange hybrids. |
| first_indexed | 2024-03-10T03:44:34Z |
| format | Article |
| id | doaj.art-89d505eaf51b4778aa972388973b228c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:34Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-89d505eaf51b4778aa972388973b228c2023-11-23T11:22:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310545710.3390/ijms23105457Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality FormationXiaoyi Bi0Ling Liao1Lijun Deng2Zhenghua Jin3Zehao Huang4Guochao Sun5Bo Xiong6Zhihui Wang7College of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaTangor, an important citrus type, is a hybrid of orange and mandarin and possesses their advantageous characteristics. Fruit quality is an important factor limiting the development of the citrus industry and highly depends on fruit development and ripening programs. However, fruit development and quality formation have not been completely explored in mandarin-orange hybrids. We sequenced the metabolome and transcriptome of three mandarin-orange hybrid cultivars at the early fruiting [90 days after full bloom (DAFB)], color change (180 DAFB), and ripening (270 DAFB) stages. Metabolome sequencing was performed to preliminarily identify the accumulation patterns of primary and secondary metabolites related to fruit quality and hormones regulating fruit development. Transcriptome analysis showed that many genes related to primary metabolism, secondary metabolism, cell wall metabolism, phytohormones, and transcriptional regulation were up-regulated in all three cultivars during fruit development and ripening. Additionally, multiple key genes were identified that may play a role in sucrose, citric acid and flavonoid accumulation, cell wall modification, and abscisic acid signaling, which may provide a valuable resource for future research on enhancement of fruit quality of hybrid citrus. Overall, this study provides new insights into the molecular basis of pulp growth and development regulation and fruit quality formation in mandarin-orange hybrids.https://www.mdpi.com/1422-0067/23/10/5457mandarin-orange hybridsfruit developmentquality formationmetabolometranscriptome |
| spellingShingle | Xiaoyi Bi Ling Liao Lijun Deng Zhenghua Jin Zehao Huang Guochao Sun Bo Xiong Zhihui Wang Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation International Journal of Molecular Sciences mandarin-orange hybrids fruit development quality formation metabolome transcriptome |
| title | Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation |
| title_full | Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation |
| title_fullStr | Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation |
| title_full_unstemmed | Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation |
| title_short | Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (<i>Citrus reticulata × Citrus sinensis</i>) Fruit Development and Quality Formation |
| title_sort | combined transcriptome and metabolome analyses reveal candidate genes involved in tangor i citrus reticulata citrus sinensis i fruit development and quality formation |
| topic | mandarin-orange hybrids fruit development quality formation metabolome transcriptome |
| url | https://www.mdpi.com/1422-0067/23/10/5457 |
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