Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival
Grafts of first trimester fetal tissue show limited survival and integration in the adult CNS. Alternative grafting strategies have been sought for treatment of neurodegenerative disease. We have developed cultures of human second trimester fetal tissues to study neuronal differentiation. Grafted in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
1999-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979900800115 |
| _version_ | 1811299347465240576 |
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| author | Michael G. White Robert R. Hammond Virginia J. Sanders Eugene A. Bonaroti Anil P. Mehta Guoji Wang Clayton A. Wiley Cristian L. Achim |
| author_facet | Michael G. White Robert R. Hammond Virginia J. Sanders Eugene A. Bonaroti Anil P. Mehta Guoji Wang Clayton A. Wiley Cristian L. Achim |
| author_sort | Michael G. White |
| collection | DOAJ |
| description | Grafts of first trimester fetal tissue show limited survival and integration in the adult CNS. Alternative grafting strategies have been sought for treatment of neurodegenerative disease. We have developed cultures of human second trimester fetal tissues to study neuronal differentiation. Grafted into the SCID mouse striatum, aggregates of these cultures formed neuron-rich xenografts for at least 8 months. We examined the influence of various neurotrophic factors, including basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), transforming growth factor-beta 1 (TGF-β1), and hepatocyte growth factor (HGF), on the growth and differentiation of neuronal and glial cell populations. BDNF promoted the survival and differentiation of second trimester neurons whereas bFGF exhibited a strong proliferative effect on precursors and the astroglial population. Our data suggest that second trimester human fetal cultures contain neuroprogenitor cells that can be directed to the neuronal lineage. This process may be amplified by treatment with BDNF, which we hypothesize could improve the long-term in vivo survival of neuron-enriched grafts. |
| first_indexed | 2024-04-13T06:33:51Z |
| format | Article |
| id | doaj.art-89d6f7ca2ff541ada5845d89a98bc4a5 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-04-13T06:33:51Z |
| publishDate | 1999-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-89d6f7ca2ff541ada5845d89a98bc4a52022-12-22T02:57:59ZengSAGE PublishingCell Transplantation0963-68971555-38921999-01-01810.1177/096368979900800115Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft SurvivalMichael G. White0Robert R. Hammond1Virginia J. Sanders2Eugene A. Bonaroti3Anil P. Mehta4Guoji Wang5Clayton A. Wiley6Cristian L. Achim7University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213University of Western Ontario, Department of Pathology, London, CanadaUniversity of California at San Diego, Department of Neurosciences, La Jolla, CA 92093University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213University of Pittsburgh School of Medicine, Department of Pathology, Division of Neuropathology, Pittsburgh, PA 15213Grafts of first trimester fetal tissue show limited survival and integration in the adult CNS. Alternative grafting strategies have been sought for treatment of neurodegenerative disease. We have developed cultures of human second trimester fetal tissues to study neuronal differentiation. Grafted into the SCID mouse striatum, aggregates of these cultures formed neuron-rich xenografts for at least 8 months. We examined the influence of various neurotrophic factors, including basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), transforming growth factor-beta 1 (TGF-β1), and hepatocyte growth factor (HGF), on the growth and differentiation of neuronal and glial cell populations. BDNF promoted the survival and differentiation of second trimester neurons whereas bFGF exhibited a strong proliferative effect on precursors and the astroglial population. Our data suggest that second trimester human fetal cultures contain neuroprogenitor cells that can be directed to the neuronal lineage. This process may be amplified by treatment with BDNF, which we hypothesize could improve the long-term in vivo survival of neuron-enriched grafts.https://doi.org/10.1177/096368979900800115 |
| spellingShingle | Michael G. White Robert R. Hammond Virginia J. Sanders Eugene A. Bonaroti Anil P. Mehta Guoji Wang Clayton A. Wiley Cristian L. Achim Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival Cell Transplantation |
| title | Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival |
| title_full | Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival |
| title_fullStr | Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival |
| title_full_unstemmed | Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival |
| title_short | Neuron-Enriched Second Trimester Human Cultures: Growth Factor Response and in Vivo Graft Survival |
| title_sort | neuron enriched second trimester human cultures growth factor response and in vivo graft survival |
| url | https://doi.org/10.1177/096368979900800115 |
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