OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice
Abstract Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB intr...
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| Format: | Article |
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BMC
2020-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02593-z |
| _version_ | 1818159502765064192 |
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| author | Lan Shen Qiang Zhang Zhongwei Wang Hongling Wen Guanglian Hu Deyong Ren Jiang Hu Li Zhu Zhenyu Gao Guangheng Zhang Longbiao Guo Dali Zeng Qian Qian |
| author_facet | Lan Shen Qiang Zhang Zhongwei Wang Hongling Wen Guanglian Hu Deyong Ren Jiang Hu Li Zhu Zhenyu Gao Guangheng Zhang Longbiao Guo Dali Zeng Qian Qian |
| author_sort | Lan Shen |
| collection | DOAJ |
| description | Abstract Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize and Arabidopsis thaliana. However, the research of the OsCAF2 gene in rice is very little, and the effects of OsCAF2 genes on chloroplasts development are not well characterized. Results In this study, oscaf2 mutants were obtained by editing the OsCAF2 gene in the Nipponbare variety of rice. Phenotypic analysis showed that mutations to OsCAF2 led to albino leaves at the seeding stage that eventually caused plant death, and oscaf2 mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated that OsCAF2 might participate in the splicing of group IIA and IIB introns, which differs from its orthologs in A. thaliana and maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers. Conclusion Taken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice. |
| first_indexed | 2024-12-11T15:47:00Z |
| format | Article |
| id | doaj.art-89d79eb92dca441c96eb066f7d856342 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T15:47:00Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-89d79eb92dca441c96eb066f7d8563422022-12-22T00:59:41ZengBMCBMC Plant Biology1471-22292020-08-0120111110.1186/s12870-020-02593-zOsCAF2 contains two CRM domains and is necessary for chloroplast development in riceLan Shen0Qiang Zhang1Zhongwei Wang2Hongling Wen3Guanglian Hu4Deyong Ren5Jiang Hu6Li Zhu7Zhenyu Gao8Guangheng Zhang9Longbiao Guo10Dali Zeng11Qian Qian12State Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesBiotechnology Research Center, Chongqing Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Rice Biology / China National Rice Research Institute, Chinese Academy of Agricultural SciencesAbstract Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize and Arabidopsis thaliana. However, the research of the OsCAF2 gene in rice is very little, and the effects of OsCAF2 genes on chloroplasts development are not well characterized. Results In this study, oscaf2 mutants were obtained by editing the OsCAF2 gene in the Nipponbare variety of rice. Phenotypic analysis showed that mutations to OsCAF2 led to albino leaves at the seeding stage that eventually caused plant death, and oscaf2 mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated that OsCAF2 might participate in the splicing of group IIA and IIB introns, which differs from its orthologs in A. thaliana and maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers. Conclusion Taken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice.http://link.springer.com/article/10.1186/s12870-020-02593-zOsCAF2CRM domainIntron splicingChloroplast developmentRice |
| spellingShingle | Lan Shen Qiang Zhang Zhongwei Wang Hongling Wen Guanglian Hu Deyong Ren Jiang Hu Li Zhu Zhenyu Gao Guangheng Zhang Longbiao Guo Dali Zeng Qian Qian OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice BMC Plant Biology OsCAF2 CRM domain Intron splicing Chloroplast development Rice |
| title | OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice |
| title_full | OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice |
| title_fullStr | OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice |
| title_full_unstemmed | OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice |
| title_short | OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice |
| title_sort | oscaf2 contains two crm domains and is necessary for chloroplast development in rice |
| topic | OsCAF2 CRM domain Intron splicing Chloroplast development Rice |
| url | http://link.springer.com/article/10.1186/s12870-020-02593-z |
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