Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells
Noninvasive targeted visualization of pancreatic beta cells or islets is becoming the focus of molecular imaging application in diabetes and islet transplantation studies. In this study, we aimed to produce the beta-cell-targeted peptide for molecular imaging of islet. We used phage display librarie...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2286 |
| _version_ | 1797438325406040064 |
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| author | Qianwen Wang Lei Zheng Kangze Wu Bo Zhang |
| author_facet | Qianwen Wang Lei Zheng Kangze Wu Bo Zhang |
| author_sort | Qianwen Wang |
| collection | DOAJ |
| description | Noninvasive targeted visualization of pancreatic beta cells or islets is becoming the focus of molecular imaging application in diabetes and islet transplantation studies. In this study, we aimed to produce the beta-cell-targeted peptide for molecular imaging of islet. We used phage display libraries to screen a beta-cell-targeted peptide, LNTPLKS, which was tagged with fluorescein isothiocyanate (FITC). This peptide was validated for targeting beta-cell with in vitro and in vivo studies. Immunocytochemistry (ICC) and fluorescence-activated cell sorting (FACS) analysis were used to validate the target specificity of the peptide. FITC-LNTPLKS displayed much higher fluorescence in beta cells vs. control cells in ICC. This discrimination was consistently observed using primary rodent islet. FACS analysis showed right shift of peak point in beta cells compared to control cells. The specific bind to in situ islet was verified by in vitro experiments using rodent and human pancreatic slices. The peptide also showed high affinity of islet grafts under the renal capsule. In the insulinoma animal model, we could find FITC-LNTPLKS accumulated specifically to the tumor, thus indicating a potential clinical application of molecular imaging of insulinoma. In conclusion, LNTPLKS showed a specific probe for beta-cells, which might be further utilized in targeted imaging/monitoring beta cells and theragnosis for beta-cells-related disease (diabetes, insulinoma, etc.). |
| first_indexed | 2024-03-09T11:36:02Z |
| format | Article |
| id | doaj.art-89dec3b8bb3047549d6e7a418cd691e8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:36:02Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-89dec3b8bb3047549d6e7a418cd691e82023-11-30T23:42:09ZengMDPI AGMolecules1420-30492022-03-01277228610.3390/molecules27072286Identification and Validation of a New Peptide Targeting Pancreatic Beta CellsQianwen Wang0Lei Zheng1Kangze Wu2Bo Zhang3Department of Surgery, Fourth Affiliated Hospital, School of Medicine, Zhejiang University, Yiwu 322000, ChinaDepartment of Emergency Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266000, ChinaDepartment of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, ChinaDepartment of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, ChinaNoninvasive targeted visualization of pancreatic beta cells or islets is becoming the focus of molecular imaging application in diabetes and islet transplantation studies. In this study, we aimed to produce the beta-cell-targeted peptide for molecular imaging of islet. We used phage display libraries to screen a beta-cell-targeted peptide, LNTPLKS, which was tagged with fluorescein isothiocyanate (FITC). This peptide was validated for targeting beta-cell with in vitro and in vivo studies. Immunocytochemistry (ICC) and fluorescence-activated cell sorting (FACS) analysis were used to validate the target specificity of the peptide. FITC-LNTPLKS displayed much higher fluorescence in beta cells vs. control cells in ICC. This discrimination was consistently observed using primary rodent islet. FACS analysis showed right shift of peak point in beta cells compared to control cells. The specific bind to in situ islet was verified by in vitro experiments using rodent and human pancreatic slices. The peptide also showed high affinity of islet grafts under the renal capsule. In the insulinoma animal model, we could find FITC-LNTPLKS accumulated specifically to the tumor, thus indicating a potential clinical application of molecular imaging of insulinoma. In conclusion, LNTPLKS showed a specific probe for beta-cells, which might be further utilized in targeted imaging/monitoring beta cells and theragnosis for beta-cells-related disease (diabetes, insulinoma, etc.).https://www.mdpi.com/1420-3049/27/7/2286phage displaytargeting peptidepancreatic beta cellsoptical imaging |
| spellingShingle | Qianwen Wang Lei Zheng Kangze Wu Bo Zhang Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells Molecules phage display targeting peptide pancreatic beta cells optical imaging |
| title | Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells |
| title_full | Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells |
| title_fullStr | Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells |
| title_full_unstemmed | Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells |
| title_short | Identification and Validation of a New Peptide Targeting Pancreatic Beta Cells |
| title_sort | identification and validation of a new peptide targeting pancreatic beta cells |
| topic | phage display targeting peptide pancreatic beta cells optical imaging |
| url | https://www.mdpi.com/1420-3049/27/7/2286 |
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