Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress
Abiotic stress is the focus of research on passion fruit characters because of its damage to the industry. Basic helix-loop-helix (bHLH) is one of the Transcription factors (TFs) which can act in an anti-abiotic stress role through diverse biological processes. However, no systemic analysis of the p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/2/272 |
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| author | Yi Xu Weidong Zhou Funing Ma Dongmei Huang Wenting Xing Bin Wu Peiguang Sun Di Chen Binqiang Xu Shun Song |
| author_facet | Yi Xu Weidong Zhou Funing Ma Dongmei Huang Wenting Xing Bin Wu Peiguang Sun Di Chen Binqiang Xu Shun Song |
| author_sort | Yi Xu |
| collection | DOAJ |
| description | Abiotic stress is the focus of research on passion fruit characters because of its damage to the industry. Basic helix-loop-helix (bHLH) is one of the Transcription factors (TFs) which can act in an anti-abiotic stress role through diverse biological processes. However, no systemic analysis of the passion fruit bHLH (PebHLH) family was reported. In this study, 117 PebHLH members were identified from the genome of passion fruit, related to plant stress resistance and development by prediction of protein interaction. Furthermore, the transcriptome sequencing results showed that the <i>PebHLHs</i> responded to different abiotic stresses. At different ripening stages of passion fruit, the expression level of most <i>PebHLHs</i> in the immature stage (T1) was higher than that in the mature stage (T2 and T3). Eight <i>PebHLHs</i> with differentially expressed under different stress treatments and different ripening stages were selected and verified by qRT-PCR. In this research, the expression of one member, <i>PebHLH56,</i> was induced under cold stress. Further, the promoter of <i>PebHLH56</i> was fused to β-Galactosidase (GUS) to generate the expression vector that was transformed into <i>Arabidopsis</i>. It showed that <i>PebHLH56</i> could significantly respond to cold stress. This study provided new insights into the regulatory functions of <i>PebHLH</i> genes during fruit maturity stages and abiotic stress, thereby improving the understanding of the characteristics and evolution of the <i>PebHLH</i> gene family. |
| first_indexed | 2024-03-11T08:45:05Z |
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| id | doaj.art-89e1f66eb9384969bfd493245e65bd3d |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T08:45:05Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-89e1f66eb9384969bfd493245e65bd3d2023-11-16T20:51:00ZengMDPI AGHorticulturae2311-75242023-02-019227210.3390/horticulturae9020272Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold StressYi Xu0Weidong Zhou1Funing Ma2Dongmei Huang3Wenting Xing4Bin Wu5Peiguang Sun6Di Chen7Binqiang Xu8Shun Song9State Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaState Key Laboratory of Biological Breeding for Tropical Crops, Haikou Experimental Station, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaAbiotic stress is the focus of research on passion fruit characters because of its damage to the industry. Basic helix-loop-helix (bHLH) is one of the Transcription factors (TFs) which can act in an anti-abiotic stress role through diverse biological processes. However, no systemic analysis of the passion fruit bHLH (PebHLH) family was reported. In this study, 117 PebHLH members were identified from the genome of passion fruit, related to plant stress resistance and development by prediction of protein interaction. Furthermore, the transcriptome sequencing results showed that the <i>PebHLHs</i> responded to different abiotic stresses. At different ripening stages of passion fruit, the expression level of most <i>PebHLHs</i> in the immature stage (T1) was higher than that in the mature stage (T2 and T3). Eight <i>PebHLHs</i> with differentially expressed under different stress treatments and different ripening stages were selected and verified by qRT-PCR. In this research, the expression of one member, <i>PebHLH56,</i> was induced under cold stress. Further, the promoter of <i>PebHLH56</i> was fused to β-Galactosidase (GUS) to generate the expression vector that was transformed into <i>Arabidopsis</i>. It showed that <i>PebHLH56</i> could significantly respond to cold stress. This study provided new insights into the regulatory functions of <i>PebHLH</i> genes during fruit maturity stages and abiotic stress, thereby improving the understanding of the characteristics and evolution of the <i>PebHLH</i> gene family.https://www.mdpi.com/2311-7524/9/2/272bHLHpassion fruitabiotic stresscoldgenome-wide analysis |
| spellingShingle | Yi Xu Weidong Zhou Funing Ma Dongmei Huang Wenting Xing Bin Wu Peiguang Sun Di Chen Binqiang Xu Shun Song Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress Horticulturae bHLH passion fruit abiotic stress cold genome-wide analysis |
| title | Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress |
| title_full | Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress |
| title_fullStr | Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress |
| title_full_unstemmed | Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress |
| title_short | Characterization of the Passion Fruit (<i>Passiflora edulis</i> Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of <i>PebHLH56</i> in Cold Stress |
| title_sort | characterization of the passion fruit i passiflora edulis i sim bhlh family in fruit development and abiotic stress and functional analysis of i pebhlh56 i in cold stress |
| topic | bHLH passion fruit abiotic stress cold genome-wide analysis |
| url | https://www.mdpi.com/2311-7524/9/2/272 |
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