Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors
Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Regenerative Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320418300324 |
| _version_ | 1818821583766028288 |
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| author | Koichiro Nishino Yoshikazu Arai Ken Takasawa Masashi Toyoda Mayu Yamazaki-Inoue Tohru Sugawara Hidenori Akutsu Ken Nishimura Manami Ohtaka Mahito Nakanishi Akihiro Umezawa |
| author_facet | Koichiro Nishino Yoshikazu Arai Ken Takasawa Masashi Toyoda Mayu Yamazaki-Inoue Tohru Sugawara Hidenori Akutsu Ken Nishimura Manami Ohtaka Mahito Nakanishi Akihiro Umezawa |
| author_sort | Koichiro Nishino |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hypermethylated regions compared with embryonic stem cells. These random aberrant hypermethylations might be a cause of the differences in the properties of each of the iPSC lines. Keywords: Human iPSCs, Human ESCs, DNA methylation, Aberrant methylation |
| first_indexed | 2024-12-18T23:10:30Z |
| format | Article |
| id | doaj.art-89e5fef2aed349748299b4161679e9d0 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-18T23:10:30Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-89e5fef2aed349748299b4161679e9d02022-12-21T20:48:22ZengElsevierRegenerative Therapy2352-32042018-12-0197178Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectorsKoichiro Nishino0Yoshikazu Arai1Ken Takasawa2Masashi Toyoda3Mayu Yamazaki-Inoue4Tohru Sugawara5Hidenori Akutsu6Ken Nishimura7Manami Ohtaka8Mahito Nakanishi9Akihiro Umezawa10Laboratory of Veterinary Biochemistry and Molecular Biology, Graduate School of Medicine and Veterinary Medicine/Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Center for Animal Disease Control, University of Miyazaki, Miyazaki, JapanLaboratory of Veterinary Biochemistry and Molecular Biology, Graduate School of Medicine and Veterinary Medicine/Faculty of Agriculture, University of Miyazaki, Miyazaki, JapanLaboratory of Veterinary Biochemistry and Molecular Biology, Graduate School of Medicine and Veterinary Medicine/Faculty of Agriculture, University of Miyazaki, Miyazaki, JapanResearch Team for Geriatric Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, Tokyo, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, Tokyo, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, Tokyo, JapanLaboratory of Gene Regulation, Faculty of Medicine, University of Tsukuba, Ibaraki, JapanTOKIWA-Bio, Inc., Ibaraki, JapanBiotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, JapanDepartment of Reproductive Biology, Center for Regenerative Medicine, National Research Institute for Child Health and Development, Tokyo, Japan; Corresponding author. Fax: +81 3 5494 7048.Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hypermethylated regions compared with embryonic stem cells. These random aberrant hypermethylations might be a cause of the differences in the properties of each of the iPSC lines. Keywords: Human iPSCs, Human ESCs, DNA methylation, Aberrant methylationhttp://www.sciencedirect.com/science/article/pii/S2352320418300324 |
| spellingShingle | Koichiro Nishino Yoshikazu Arai Ken Takasawa Masashi Toyoda Mayu Yamazaki-Inoue Tohru Sugawara Hidenori Akutsu Ken Nishimura Manami Ohtaka Mahito Nakanishi Akihiro Umezawa Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors Regenerative Therapy |
| title | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
| title_full | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
| title_fullStr | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
| title_full_unstemmed | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
| title_short | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
| title_sort | epigenetic scale comparison of human ipscs generated by retrovirus sendai virus or episomal vectors |
| url | http://www.sciencedirect.com/science/article/pii/S2352320418300324 |
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