Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue
Objective: Uterine myoma is the most common benign reproductive tract tumor in women. Despite its high prevalence, the exact pathogenesis of these benign tumors remains unknown. Toward understanding the pathogenic mechanism of these tumors, we attempted to isolate human uterine myoma mesenchymal ste...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-06-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455913000727 |
| _version_ | 1818344173499056128 |
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| author | Kai-Hung Wang An-Pei Kao Chia-Cheng Chang Ta-Chin Lin Tsung-Cheng Kuo |
| author_facet | Kai-Hung Wang An-Pei Kao Chia-Cheng Chang Ta-Chin Lin Tsung-Cheng Kuo |
| author_sort | Kai-Hung Wang |
| collection | DOAJ |
| description | Objective: Uterine myoma is the most common benign reproductive tract tumor in women. Despite its high prevalence, the exact pathogenesis of these benign tumors remains unknown. Toward understanding the pathogenic mechanism of these tumors, we attempted to isolate human uterine myoma mesenchymal stem cells (hUM-MSCs), which may be the target cells for tumorigenesis. Furthermore, we tested the response of these hUM-MSCs to the environmental endocrine disruptor, bisphenol A (BPA), which may mimic the action of estrogen in hormone-sensitive organs such as the uterus.
Materials and Methods: The hUM-MSC lines were clonally derived from uterine myoma tissue using the MSU-1 medium supplemented with N-acetyl-l-cysteine and l-ascorbic acid-2-phosphate. These hUM-MSCs were characterized by reverse transcription polymerase chain reaction (RT-PCR) analysis for the expression of mesenchymal stem cell (MSC) surface markers (e.g., CD90 and CD105) and the transcription factor Oct-4. The proliferation potential was measured by the cumulative population doubling level and the colony-forming efficiency.
Results: Putative hUM-MSC lines expressed CD90, CD105, and the stem cell marker gene, Oct-4. The cells were capable of differentiating into adipocytes, osteoblasts, and chondrocytes. Bisphenol A treatment of these hUM-MSCs enhanced cell proliferation and colony-forming efficiency in a dose-responsive manner. At an environmentally relevant concentration (10−8 M), BPA moreover induced cyclooxygenase-2 (COX-2) gene expression and promoted cell migration and invasiveness.
Conclusion: The hUM-MSC cell lines can be isolated from uterine myoma tissues. Bisphenol A could enhance cell proliferation and colony-forming efficiency, induce COX-2 gene expression, and promote migration and invasion of hUM-MSCs. The results imply that BPA has a detrimental effect on female health by promoting uterine tumorigenesis. |
| first_indexed | 2024-12-13T16:42:16Z |
| format | Article |
| id | doaj.art-89e7c62f23544b9fae807c036fbe0d0f |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-13T16:42:16Z |
| publishDate | 2013-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-89e7c62f23544b9fae807c036fbe0d0f2022-12-21T23:38:15ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592013-06-0152224625210.1016/j.tjog.2013.04.016Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissueKai-Hung Wang0An-Pei Kao1Chia-Cheng Chang2Ta-Chin Lin3Tsung-Cheng Kuo4Department of Obstetrics and Gynecology, Kuo General Hospital, Tainan, TaiwanDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine and Sciences, Taipei Medical University Hospital, Taipei Medical University, Taipei, TaiwanDepartment of Pediatrics and Human Development, Michigan State University, East Lansing, MI 48824, USADepartment of Obstetrics and Gynecology, Kuo General Hospital, Tainan, TaiwanDepartment of Obstetrics and Gynecology, Kuo General Hospital, Tainan, TaiwanObjective: Uterine myoma is the most common benign reproductive tract tumor in women. Despite its high prevalence, the exact pathogenesis of these benign tumors remains unknown. Toward understanding the pathogenic mechanism of these tumors, we attempted to isolate human uterine myoma mesenchymal stem cells (hUM-MSCs), which may be the target cells for tumorigenesis. Furthermore, we tested the response of these hUM-MSCs to the environmental endocrine disruptor, bisphenol A (BPA), which may mimic the action of estrogen in hormone-sensitive organs such as the uterus. Materials and Methods: The hUM-MSC lines were clonally derived from uterine myoma tissue using the MSU-1 medium supplemented with N-acetyl-l-cysteine and l-ascorbic acid-2-phosphate. These hUM-MSCs were characterized by reverse transcription polymerase chain reaction (RT-PCR) analysis for the expression of mesenchymal stem cell (MSC) surface markers (e.g., CD90 and CD105) and the transcription factor Oct-4. The proliferation potential was measured by the cumulative population doubling level and the colony-forming efficiency. Results: Putative hUM-MSC lines expressed CD90, CD105, and the stem cell marker gene, Oct-4. The cells were capable of differentiating into adipocytes, osteoblasts, and chondrocytes. Bisphenol A treatment of these hUM-MSCs enhanced cell proliferation and colony-forming efficiency in a dose-responsive manner. At an environmentally relevant concentration (10−8 M), BPA moreover induced cyclooxygenase-2 (COX-2) gene expression and promoted cell migration and invasiveness. Conclusion: The hUM-MSC cell lines can be isolated from uterine myoma tissues. Bisphenol A could enhance cell proliferation and colony-forming efficiency, induce COX-2 gene expression, and promote migration and invasion of hUM-MSCs. The results imply that BPA has a detrimental effect on female health by promoting uterine tumorigenesis.http://www.sciencedirect.com/science/article/pii/S1028455913000727bisphenol Acell invasioncyclooxygenase-2mesenchymal stem cellsuterine myoma |
| spellingShingle | Kai-Hung Wang An-Pei Kao Chia-Cheng Chang Ta-Chin Lin Tsung-Cheng Kuo Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue Taiwanese Journal of Obstetrics & Gynecology bisphenol A cell invasion cyclooxygenase-2 mesenchymal stem cells uterine myoma |
| title | Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| title_full | Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| title_fullStr | Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| title_full_unstemmed | Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| title_short | Bisphenol A at environmentally relevant doses induces cyclooxygenase-2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| title_sort | bisphenol a at environmentally relevant doses induces cyclooxygenase 2 expression and promotes invasion of human mesenchymal stem cells derived from uterine myoma tissue |
| topic | bisphenol A cell invasion cyclooxygenase-2 mesenchymal stem cells uterine myoma |
| url | http://www.sciencedirect.com/science/article/pii/S1028455913000727 |
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