Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats
The current study explored whether ALA could prevent testis Pb accumulation and Pb-induced oxidative testicular toxicity in rats. Adult male rats were randomly divided and administered ALA (25 mg/kg/day) and/or Pb (Pb acetate, 20 mg/kg/day) in Control group, ALA group, Pb group, and ALA + Pb group f...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Scientific African |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227623002983 |
| _version_ | 1797676231603257344 |
|---|---|
| author | Ademola C. Famurewa Hamida Hamdi Azza Sedky |
| author_facet | Ademola C. Famurewa Hamida Hamdi Azza Sedky |
| author_sort | Ademola C. Famurewa |
| collection | DOAJ |
| description | The current study explored whether ALA could prevent testis Pb accumulation and Pb-induced oxidative testicular toxicity in rats. Adult male rats were randomly divided and administered ALA (25 mg/kg/day) and/or Pb (Pb acetate, 20 mg/kg/day) in Control group, ALA group, Pb group, and ALA + Pb group for 4 weeks consecutively. Pb exposure significantly increased Pb accumulation in the testes and blood of Pb-exposed rats compared to the control. Pb caused marked deficits in sperm count, motility along with elevated abnormal sperm cells, while serum testosterone, follicle stimulating hormone (FSH) and leutenizing hormone (LH) levels reduced prominently in comparison to the control. It remarkably depressed the testicular activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), as well as levels of interleukin-4 (IL-4) and interleukin-10 (IL-10), whereas malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) increased significantly compared to the control. The levels of caspase-3, capsase-9, and the PCR gene expression of Bax and Bcl2 were adversely altered with alterations in testis histology. Interestingly, the co-treatment with ALA blocks Pb accumulation in the testis and blood. It inhibited the Pb-induced alterations in reproductive indices and reversed the oxidative stress-mediated inflammatory stress, apoptotic signaling and histopathological abrasions. The findings reveal that ALA could abrogate Pb-induced testicular toxicity through blocking testicular oxidative inflammation and apoptosis in rats. |
| first_indexed | 2024-03-11T22:25:59Z |
| format | Article |
| id | doaj.art-89e934fd18f94181a1671bee71f6872f |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-03-11T22:25:59Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-89e934fd18f94181a1671bee71f6872f2023-09-24T05:16:19ZengElsevierScientific African2468-22762023-09-0121e01842Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in ratsAdemola C. Famurewa0Hamida Hamdi1Azza Sedky2Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medical Sciences, Alex Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka State, India; Corresponding author at: Department of Medical Biochemistry, Faculty of Basic Medical, Sciences, College of Medical Sciences, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria.Department of Biology, College of Science, Taif University, Taif, 21944, Saudi ArabiaDepartment of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia; Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; Corresponding author at: Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia.The current study explored whether ALA could prevent testis Pb accumulation and Pb-induced oxidative testicular toxicity in rats. Adult male rats were randomly divided and administered ALA (25 mg/kg/day) and/or Pb (Pb acetate, 20 mg/kg/day) in Control group, ALA group, Pb group, and ALA + Pb group for 4 weeks consecutively. Pb exposure significantly increased Pb accumulation in the testes and blood of Pb-exposed rats compared to the control. Pb caused marked deficits in sperm count, motility along with elevated abnormal sperm cells, while serum testosterone, follicle stimulating hormone (FSH) and leutenizing hormone (LH) levels reduced prominently in comparison to the control. It remarkably depressed the testicular activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), as well as levels of interleukin-4 (IL-4) and interleukin-10 (IL-10), whereas malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) increased significantly compared to the control. The levels of caspase-3, capsase-9, and the PCR gene expression of Bax and Bcl2 were adversely altered with alterations in testis histology. Interestingly, the co-treatment with ALA blocks Pb accumulation in the testis and blood. It inhibited the Pb-induced alterations in reproductive indices and reversed the oxidative stress-mediated inflammatory stress, apoptotic signaling and histopathological abrasions. The findings reveal that ALA could abrogate Pb-induced testicular toxicity through blocking testicular oxidative inflammation and apoptosis in rats.http://www.sciencedirect.com/science/article/pii/S2468227623002983TestisAlpha-lipoic acidBioaccumulationToxicityInflammationApoptosis |
| spellingShingle | Ademola C. Famurewa Hamida Hamdi Azza Sedky Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats Scientific African Testis Alpha-lipoic acid Bioaccumulation Toxicity Inflammation Apoptosis |
| title | Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats |
| title_full | Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats |
| title_fullStr | Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats |
| title_full_unstemmed | Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats |
| title_short | Lipoic acid abates testis lead accumulation, sperm-endocrine deficits, testicular oxidative inflammation and apoptosis and modulates gene expression of Bax and Bcl-2 in rats |
| title_sort | lipoic acid abates testis lead accumulation sperm endocrine deficits testicular oxidative inflammation and apoptosis and modulates gene expression of bax and bcl 2 in rats |
| topic | Testis Alpha-lipoic acid Bioaccumulation Toxicity Inflammation Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2468227623002983 |
| work_keys_str_mv | AT ademolacfamurewa lipoicacidabatestestisleadaccumulationspermendocrinedeficitstesticularoxidativeinflammationandapoptosisandmodulatesgeneexpressionofbaxandbcl2inrats AT hamidahamdi lipoicacidabatestestisleadaccumulationspermendocrinedeficitstesticularoxidativeinflammationandapoptosisandmodulatesgeneexpressionofbaxandbcl2inrats AT azzasedky lipoicacidabatestestisleadaccumulationspermendocrinedeficitstesticularoxidativeinflammationandapoptosisandmodulatesgeneexpressionofbaxandbcl2inrats |