rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance
Background: Allergic dermatitis (AD) is an inflammatory skin disease that arises from abnormal T lymphocyte activation. A recombinant fusion protein comprising Helicobacter pylori neutrophil-activating protein and maltose binding protein, rMBP-NAP, has been documented as a novel immunomodulatory TLR...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2023-03-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_49132_05e071d3a26efc6fc136e6bb14afeec0.pdf |
| _version_ | 1797857630791663616 |
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| author | Hao Zheng Qiaozhen Kang Chenglong Zhang Lu Yang Xin Liu |
| author_facet | Hao Zheng Qiaozhen Kang Chenglong Zhang Lu Yang Xin Liu |
| author_sort | Hao Zheng |
| collection | DOAJ |
| description | Background: Allergic dermatitis (AD) is an inflammatory skin disease that arises from abnormal T lymphocyte activation. A recombinant fusion protein comprising Helicobacter pylori neutrophil-activating protein and maltose binding protein, rMBP-NAP, has been documented as a novel immunomodulatory TLR agonist.Objective: To explore the effect of the rMBP-NAP on the OXA-induced AD in a mouse model and clarify the possible action mechanism.Methods: The AD animal model was induced by repeated administration of oxazolone (OXA) in BALB/c mice. H&E staining was used to analyze the ear epidermis thickness and the number of infiltrating inflammatory cells. TB staining was used to detect mast cell infiltration in the ear tissue. ELISA was used to analyze the secretion of cytokines IL-4 and IFN-γ in peripheral blood. qRT-PCR was used to determine the expression levels of IL-4, IFN-γ, and IL-13 in ear tissue.Results: OXA induced the establishment of an AD model. After the rMBP-NAP treatment, the thickness of the ear tissue and the number of mast cells infiltrated in AD mice reduced, and the serum and ear tissue levels of IL-4 and IFN-γ increased, but the ratio of IFN-γ (rMBP-NAP group)/IL-4 (rMBP-NAP group) was greater than the ratio of IFN-γ (sensitized group)/IL-4 (sensitized group).Conclusion: The rMBP-NAP improved the disease symptoms including skin lesions in AD, alleviated the inflammation in ear tissue, and restored the Th1/2 balance by inducing a shift from the Th2 to the Th1 response. The results of our work support the use of rMBP-NAP as an immunomodulator for AD treatment in future investigations. |
| first_indexed | 2024-04-09T20:59:48Z |
| format | Article |
| id | doaj.art-89eab1c010114ecc976db2233ab90476 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-04-09T20:59:48Z |
| publishDate | 2023-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-89eab1c010114ecc976db2233ab904762023-03-29T10:06:37ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2023-03-01201364410.22034/iji.2023.92594.215749132rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 BalanceHao Zheng0Qiaozhen Kang1Chenglong Zhang2Lu Yang3Xin Liu4School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.Background: Allergic dermatitis (AD) is an inflammatory skin disease that arises from abnormal T lymphocyte activation. A recombinant fusion protein comprising Helicobacter pylori neutrophil-activating protein and maltose binding protein, rMBP-NAP, has been documented as a novel immunomodulatory TLR agonist.Objective: To explore the effect of the rMBP-NAP on the OXA-induced AD in a mouse model and clarify the possible action mechanism.Methods: The AD animal model was induced by repeated administration of oxazolone (OXA) in BALB/c mice. H&E staining was used to analyze the ear epidermis thickness and the number of infiltrating inflammatory cells. TB staining was used to detect mast cell infiltration in the ear tissue. ELISA was used to analyze the secretion of cytokines IL-4 and IFN-γ in peripheral blood. qRT-PCR was used to determine the expression levels of IL-4, IFN-γ, and IL-13 in ear tissue.Results: OXA induced the establishment of an AD model. After the rMBP-NAP treatment, the thickness of the ear tissue and the number of mast cells infiltrated in AD mice reduced, and the serum and ear tissue levels of IL-4 and IFN-γ increased, but the ratio of IFN-γ (rMBP-NAP group)/IL-4 (rMBP-NAP group) was greater than the ratio of IFN-γ (sensitized group)/IL-4 (sensitized group).Conclusion: The rMBP-NAP improved the disease symptoms including skin lesions in AD, alleviated the inflammation in ear tissue, and restored the Th1/2 balance by inducing a shift from the Th2 to the Th1 response. The results of our work support the use of rMBP-NAP as an immunomodulator for AD treatment in future investigations.https://iji.sums.ac.ir/article_49132_05e071d3a26efc6fc136e6bb14afeec0.pdfallergic dermatitisimmunomodulationrmbp-napth1/th2 |
| spellingShingle | Hao Zheng Qiaozhen Kang Chenglong Zhang Lu Yang Xin Liu rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance Iranian Journal of Immunology allergic dermatitis immunomodulation rmbp-nap th1/th2 |
| title | rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance |
| title_full | rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance |
| title_fullStr | rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance |
| title_full_unstemmed | rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance |
| title_short | rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance |
| title_sort | rmbp nap suppresses oxa induced allergic dermatitis by regulating the th1 th2 balance |
| topic | allergic dermatitis immunomodulation rmbp-nap th1/th2 |
| url | https://iji.sums.ac.ir/article_49132_05e071d3a26efc6fc136e6bb14afeec0.pdf |
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