Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast
Abstract Background Probiotics have recently been applied in aquaculture as eco-friendly alternatives to antibiotics to improve fish health, simultaneously with the increase of production parameters. The present study aimed to investigate the functional potential of lactic acid bacteria (LAB) isolat...
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BMC
2023-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02899-6 |
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| author | Wahauwouélé Hermann Coulibaly N’goran Richard Kouadio Fatoumata Camara Camelia Diguță Florentina Matei |
| author_facet | Wahauwouélé Hermann Coulibaly N’goran Richard Kouadio Fatoumata Camara Camelia Diguță Florentina Matei |
| author_sort | Wahauwouélé Hermann Coulibaly |
| collection | DOAJ |
| description | Abstract Background Probiotics have recently been applied in aquaculture as eco-friendly alternatives to antibiotics to improve fish health, simultaneously with the increase of production parameters. The present study aimed to investigate the functional potential of lactic acid bacteria (LAB) isolated from the gut of Tilapia (Oreochromis niloticus) originating from the aquaculture farm of Oceanologic Research Center in Ivory Coast. Results Twelve LAB strains were identified by 16 S rDNA gene sequence homology analysis belonging to two genera Pediococcus (P. acidilactici and P. pentosaceus) and Lactobacillus (L. plantarum) with a predominance of P. acidilactici. Several aspects including functional, storage, and safety characteristics were taken into consideration in the selection process of the native LAB isolates as potential probiotics. All LAB isolates showed high antagonistic activity against bacterial pathogens like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus. In addition, the LAB isolates exhibited different degrees of cell surface hydrophobicity in the presence of hexane, xylene, and chloroform as solvents and a good ability to form biofilm. The strong antioxidant activity expressed through the DPPH scavenging capacity of LAB intact cells and their cell-free supernatants was detected. LAB strains survived between 34.18% and 49.9% when exposed to low pH (1.5) and pepsin for 3 h. In presence of 0.3% bile salts, the growth rate ranged from 0.92 to 21.46%. Antibiotic susceptibility pattern of LAB isolates showed sensitivity or intermediate resistance to amoxicillin, cephalothin, chloramphenicol, imipenem, kanamycin, penicillin, rifampicin, streptomycin, tetracycline and resistance to oxacillin, gentamicin, and ciprofloxacin. No significant difference in antibiotic susceptibility pattern was observed between P. acidilactici and P. pentosaceus strains. The non-hemolytic activity was detected. Following the analysis of the enzyme profile, the ability of LAB isolates to produce either lipase or β-galactosidase or both enzymes was highlighted. Furthermore, the efficacy of cryoprotective agents was proved to be isolate-dependent, with LAB isolates having a high affinity for D-sorbitol and sucrose. Conclusion The explored LAB strains inhibited the growth of pathogens and survived after exposure to simulated gastrointestinal tract conditions. The safety and preservative properties are desirable attributes of these new probiotic strains hence recommended for future food and feed applications. |
| first_indexed | 2024-03-13T09:03:46Z |
| format | Article |
| id | doaj.art-89eae09a964e4b10804c4499c2af995f |
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| language | English |
| last_indexed | 2024-03-13T09:03:46Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-89eae09a964e4b10804c4499c2af995f2023-05-28T11:09:57ZengBMCBMC Microbiology1471-21802023-05-0123111510.1186/s12866-023-02899-6Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory CoastWahauwouélé Hermann Coulibaly0N’goran Richard Kouadio1Fatoumata Camara2Camelia Diguță3Florentina Matei4Biotechnology and Food Microbiology Laboratory, Food Science and Technology, Formation and Research Unit, University Nangui AbrogouaNutrition and Food Safety Laboratory, Food Science and Technology, Formation and Research Unit, University Nangui AbrogouaNutrition and Food Safety Laboratory, Food Science and Technology, Formation and Research Unit, University Nangui AbrogouaApplied Microbiology Laboratory, Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine BucharestApplied Microbiology Laboratory, Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine BucharestAbstract Background Probiotics have recently been applied in aquaculture as eco-friendly alternatives to antibiotics to improve fish health, simultaneously with the increase of production parameters. The present study aimed to investigate the functional potential of lactic acid bacteria (LAB) isolated from the gut of Tilapia (Oreochromis niloticus) originating from the aquaculture farm of Oceanologic Research Center in Ivory Coast. Results Twelve LAB strains were identified by 16 S rDNA gene sequence homology analysis belonging to two genera Pediococcus (P. acidilactici and P. pentosaceus) and Lactobacillus (L. plantarum) with a predominance of P. acidilactici. Several aspects including functional, storage, and safety characteristics were taken into consideration in the selection process of the native LAB isolates as potential probiotics. All LAB isolates showed high antagonistic activity against bacterial pathogens like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus. In addition, the LAB isolates exhibited different degrees of cell surface hydrophobicity in the presence of hexane, xylene, and chloroform as solvents and a good ability to form biofilm. The strong antioxidant activity expressed through the DPPH scavenging capacity of LAB intact cells and their cell-free supernatants was detected. LAB strains survived between 34.18% and 49.9% when exposed to low pH (1.5) and pepsin for 3 h. In presence of 0.3% bile salts, the growth rate ranged from 0.92 to 21.46%. Antibiotic susceptibility pattern of LAB isolates showed sensitivity or intermediate resistance to amoxicillin, cephalothin, chloramphenicol, imipenem, kanamycin, penicillin, rifampicin, streptomycin, tetracycline and resistance to oxacillin, gentamicin, and ciprofloxacin. No significant difference in antibiotic susceptibility pattern was observed between P. acidilactici and P. pentosaceus strains. The non-hemolytic activity was detected. Following the analysis of the enzyme profile, the ability of LAB isolates to produce either lipase or β-galactosidase or both enzymes was highlighted. Furthermore, the efficacy of cryoprotective agents was proved to be isolate-dependent, with LAB isolates having a high affinity for D-sorbitol and sucrose. Conclusion The explored LAB strains inhibited the growth of pathogens and survived after exposure to simulated gastrointestinal tract conditions. The safety and preservative properties are desirable attributes of these new probiotic strains hence recommended for future food and feed applications.https://doi.org/10.1186/s12866-023-02899-6Tilapia fishPediococcus acidilacticiPediococcus pentosaceusLactobacillus plantarumProbioticsAquaculture |
| spellingShingle | Wahauwouélé Hermann Coulibaly N’goran Richard Kouadio Fatoumata Camara Camelia Diguță Florentina Matei Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast BMC Microbiology Tilapia fish Pediococcus acidilactici Pediococcus pentosaceus Lactobacillus plantarum Probiotics Aquaculture |
| title | Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast |
| title_full | Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast |
| title_fullStr | Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast |
| title_full_unstemmed | Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast |
| title_short | Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast |
| title_sort | functional properties of lactic acid bacteria isolated from tilapia oreochromis niloticus in ivory coast |
| topic | Tilapia fish Pediococcus acidilactici Pediococcus pentosaceus Lactobacillus plantarum Probiotics Aquaculture |
| url | https://doi.org/10.1186/s12866-023-02899-6 |
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